Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl

Simões, Cláudia Maria Oliveira; Albarello, Norma; Callado, Cátia Henriques; Castro, Tatiana Carvalho de; Mansur, Elisabeth

doi:10.1590/s1516-89132010000300024

Cited by 23 publications

(22 citation statements)

References 28 publications

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“…This species has been studied using plant cell culture strategies (Simões et al 2004(Simões et al , 2009a, in relation to its medicinal potential (Simões et al 2006). More recently, an anthocyanin-producing callus culture line has also been obtained (Simões et al 2009b).…”

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confidence: 99%

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Establishment of anthocyanin-producing cell suspension cultures of Cleome rosea Vahl ex DC. (Capparaceae)

Simões-Gurgel

Cordeiro

Castro

et al. 2011

Plant Cell Tiss Organ Cult

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The effects of different levels of Murashige and Skoog (MS) basal medium, 2,4-dichlorophenoxyacetic acid (2,4-D), and sucrose on anthocyanin production and biomass accumulation of cell suspension cultures of Cleome rosea were investigated. Cultures were established in liquid MS medium containing 30 g l -1 sucrose and supplemented with 0.90 lM 2,4-D. Proliferating cell suspension cultures achieved the highest growth capacity, a fourfold increase in biomass accumulation, following subculture at the exponential growth phase, 14-18 days of culture. Moreover, the presence of 2,4-D was essential for anthocyanin production and biomass accumulation. On the other hand, increasing levels of sucrose above 30 g l -1 resulted in a drastic reduction in biomass accumulation. Anthocyanin production was highest in cell suspension cultures grown on halfstrength MS medium (1/2 MS), 30 g l -1 sucrose, and 0.45 lM 2,4-D. These cell suspension cultures were mainly composed of small aggregates of spherical cells with similar morphology observed in anthocyanin-producing and non-producing cultures. Moreover, microscopic analysis of anthocyanin-producing cultures showed the presence of mixtures of non-pigmented, low-pigmented, and high-pigmented cells.

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“…Stem segments (0.5 cm) from in vitro-grown plants (Simões et al 2004) were incubated on solidified Murashige and Skoog (MS) (1962) medium containing 30 g l -1 sucrose and supplemented with 0.90 lM 2,4-dichlorophenoxyacetic acid (2,4-D). Calli were subcultured using the same medium composition by transferring samples of approximately 4 g at 20-day intervals.…”

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confidence: 99%

Establishment of anthocyanin-producing cell suspension cultures of Cleome rosea Vahl ex DC. (Capparaceae)

Simões-Gurgel

Cordeiro

Castro

et al. 2011

Plant Cell Tiss Organ Cult

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“…Cleome rosea Vahl (Capparaceae) is a Brazilian herbaceous annual species frequently found in coastal sandy plains, known as ''restingas'', which are ecosystems intensely affected by human impact. This species can be used as garden ornamental, due to its inflorescences with beautiful and attractive pink flowers and has recently been studied in regard to biotechnological (Simões et al 2004) and medicinal (Simões et al 2006) aspects.…”

Section: Introductionmentioning

confidence: 99%

Antiviral activity of Cleome rosea extracts from field-grown plants and tissue culture-derived materials against acyclovir-resistant Herpes simplex viruses type 1 (ACVr-HSV-1) and type 2 (ACVr-HSV-2)

Simões

Castro

Cordeiro

et al. 2009

World J Microbiol Biotechnol

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Extracts from Cleome rosea were investigated for their activity against acyclovir-resistant strains of Herpes simplex type 1 (ACVr-HSV-1) and type 2 (ACVr-HSV-2). Methanolic and acidified (1% (v/v) HCl) methanolic extracts were prepared from field-grown plants and in vitro propagated plants, as well as from calli and cell suspension cultures. The extracts presented low cytotoxicity and caused virus titer reduction above 70%, with different mechanisms of action. Extracts from leaves of field-grown plants inhibited viral infection mainly by affecting the virus particle itself (virucidal effect), while extracts from calli acted mainly on cell receptors. On the other hand, all extracts evaluated affected the virus entry across the cell membrane and the intracellular viral replication at similar percentages, causing reduction on titers in the range of 68-90%. This study validated the potential use of in vitro materials as sources of antiherpetic agents from C. rosea.

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“…In vitro clonal propagation is only the alternative way for the regeneration and expedites release of large number of C. viscosa plants under aseptic culture conditions. Earlier, there have been few reports on establishment of in vitro propagation through callus culture of C. viscosa [8][9], micropropagation and shoot organogenesis from different explants of Cleome spinosa [10] - [13] and Cleome gynanadra [14], somatic embryogenesis [15] cell suspension and root culture with cryopreservation of C. rosea [16]. The present study was carried out to standardize a protocol for in vitro propagation of C. gynandra from nodal explants.…”

Section: Introductionmentioning

confidence: 99%

In vitro Propagation of Cleome gynandra L. using Nodal Explants

2017

IJSR

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Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl

Abstract: This paper describes a protocol for the efficient vegetative propagation of

Cited by 23 publications

References 28 publications

Establishment of anthocyanin-producing cell suspension cultures of Cleome rosea Vahl ex DC. (Capparaceae)

Establishment of anthocyanin-producing cell suspension cultures of Cleome rosea Vahl ex DC. (Capparaceae)

Antiviral activity of Cleome rosea extracts from field-grown plants and tissue culture-derived materials against acyclovir-resistant Herpes simplex viruses type 1 (ACVr-HSV-1) and type 2 (ACVr-HSV-2)

In vitro Propagation of Cleome gynandra L. using Nodal Explants

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