Somatic embryogenesis from anthers of the autochthonous Vitis vinifera cv. Domina leads to Arabis mosaic virus-free plants

Fernández, E.; Sommerbauer, T.; Popowich, E. A.; Schartl, Angelika; Laimer, Margit

doi:10.1007/s10658-008-9404-0

Cited by 16 publications

(19 citation statements)

References 12 publications

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“…Thermotherapy, even in association with other techniques such as tissue culture, represents the most fre- Goussard & Wiid, 1992;Popescu et al, 2003Popescu et al, , 2010Gambino et al, 2006Gambino et al, , 2010Gribaudo et al, 2006;Borroto-Fernández et al, 2009;Youssef et al, 2009Ramos & Zamora, 1999D'Onghia et al, 2001;Golino et al, 2007;Quainoo et al, 2008Fitch et al, 2001Parmessur et al, 2002;Ramgareeb et al, 2010Ebi et al, 2000Ayabe & Sumi, 2001;Ramírez-Malagón et al, 2006Truskinov & Rogozina, 1997Mahmoud et al, 2009Wangai & Bock, 1996Morris et al, 1997;S edivá et al, 2006;Kumar et al, 2009;Kabir et al, 2010 quently used technique for sanitation over the last 20 years, while applications of methods such as chemotherapy and tissue culture represent the main topic in plant sanitation research.…”

Section: Comparison Of Techniquesmentioning

confidence: 99%

Review. Elimination of viruses in plants: twenty years of progress

Panattoni

Luvisi

Triolo

2013

Span. j. agric. res.

145

112

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To shed light on trends about elimination of viruses from plants, a bibliographic research was conducted to identify thermotherapy, chemotherapy and tissue culture trials published from 1991 through 2010. Among woody plants, grapevine, apple and peach are the most frequent targets of sanitation protocols because their health status is strictly regulated. Even if thermotherapy represents the preferred method for the host, grapevine viruses can also be eliminated with chemotherapy and tissue culture; apple viruses respond to chemotherapy as well. Although a similar trend was reported among herbaceous plants, chemotherapy was the most frequently used technique in potato. With regard to virus, thermotherapy was successfully applied against viruses belonging to 13 families and an unassigned genus. Instead, chemotherapy and tissue culture techniques eradicated viruses belonging to fewer families (nine). An interpretation of thermotherapy effects considers the new metabolic “pathways” triggered by the natural antiviral response emitted by the infected plant, with particular reference to virus-induced gene silencing. With regard to chemotherapy, several groups of antiviral drugs belong to inosine monophosphate dehydrogenase inhibitors, S-adenosylhomocysteine hydrolase inhibitors, neuraminidase inhibitors. Tissue culture, usually adopted to regenerate plantlets in biotechnological breeding programs, represents the less used tool for eliminate viruses from plants.

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Section: Comparison Of Techniquesmentioning

confidence: 99%

Review. Elimination of viruses in plants: twenty years of progress

Panattoni

Luvisi

Triolo

2013

Span. j. agric. res.

145

112

View full text Add to dashboard Cite

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“…Somatic embryogenesis, usually adopted to regenerate plantlets in biotechnological breeding programs, successfully eradicated viruses from Citrus (D' Onghia et al 2001), sugarcane (Parmessur et al 2002) and cacao (Quainoo et al 2008). In grapevine it efficiently eliminated several phloem-limited viruses (Goussard et al 1991;) and recently two nepovirus, GFLV (Gambino et al 2009) and Arabis mosaic virus (Borroto-Fernandez et al 2009). The mechanism whereby regenerated somatic embryos are freed of some viruses is not clear.…”

Section: Introductionmentioning

confidence: 99%

In situ localization of Grapevine fanleaf virus and phloem-restricted viruses in embryogenic callus of Vitis vinifera

Gambino¹,

Vallania²,

Gribaudo³

2010

Eur J Plant Pathol

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In grapevine, somatic embryogenesis is particularly effective in eliminating several important virus diseases. However, the mechanism whereby regenerated somatic embryos are freed of the viruses is not clear. The distribution of Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus-3 (GLRaV-3) and Grapevine virus A (GVA) in embryogenic callus of grapevine was investigated by in situ hybridization using digoxygenin-labelled oligonucleotide probes. Four months after culture initiation, in callus originated by GFLV-infected explants we observed a mosaic of infected and uninfected cells, with high concentrations of viruses in some cell groups in peripheral zones of the callus. In addition some abnormal somatic embryos showed a high hybridization signal. In callus originated by GVAand GLRaV-3-infected explants the viruses were concentrated in few cells surrounded by areas of virus-free cells. The two viruses were generally localized in different clusters of cells inside the callus and the levels of infection were lower than those observed in GFLV-infected callus. No virus was detected in callus nor in somatic embryos after 6 months of culture. The results highlight the difficulties of some viruses at stably invading callus tissues and the differential ability of GFLV to spread in the callus cells compared to the phloem-limited viruses.

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“…This period is shorter than others reported for the sanitation of virus-infected grape varieties (Gambino et al, 2006(Gambino et al, , 2009(Gambino et al, , 2011Borroto-Fernández et al, 2009).…”

Section: Discussionmentioning

confidence: 63%

“…The explants commonly used for grape embryogenesis induction are anthers and ovaries (Goussard et al, 1991;Gribaudo et al, 2004;Kikkert et al, 2005;López-Pérez et al, 2005;Gambino et al, 2006Gambino et al, , 2009Borroto-Fernández et al, 2009). We considered it interesting to test the effect of cutting the explants, so that the medium was in contact with the internal part of the explants.…”

mentioning

confidence: 99%

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

Peiró

Gammoudi

Yuste

et al. 2015

Span. j. agric. res.

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<p>The conservation of old grapevine varieties is important since they are adapted to specific climate conditions and may carry genes interesting to breeders. As virus infection is common in grapevine varieties, the use of virus free materials is of great importance. In this work, we used somatic embryogenesis for the sanitation of GLRaV-1 and GLRaV-3 viruses that were found after analyzing the putative presence of the five most common, economically important grape viruses by real-time multiplex RT-PCR in the old cultivar “Grumet Negre”. Unopened and opened inflorescences, fecundated ovaries, and, also, mature seeds were used as starting explants. Explants were<em> </em>cultured on plates with two embryogenesis induction media (Nitsch & McCown Woody plant medium) that contained the growth regulator thidiazuron and differed in their salt and vitamin compositions. One half of each kind of explant was cut prior to being cultured. After five months of culture, embryos had only developed from seeds that were cut previous to sowing. To the best of our knowledge, this is the first time that mature seeds have been used for inducing embryogenesis in grape. A total of 42% of the embryos transferred to tubes for germination regenerated into normal plantlets. The absence of both the GLRaV-1 and GLRaV-3 viruses in all regenerated plants was confirmed by real-time uniplex RT-PCR. So, this protocol can be used for sanitation and also for micropropagation.</p>

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Somatic embryogenesis from anthers of the autochthonous Vitis vinifera cv. Domina leads to Arabis mosaic virus-free plants

Cited by 16 publications

References 12 publications

Review. Elimination of viruses in plants: twenty years of progress

Review. Elimination of viruses in plants: twenty years of progress

In situ localization of Grapevine fanleaf virus and phloem-restricted viruses in embryogenic callus of Vitis vinifera

Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

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