Somatic embryogenesis from leaf and petiole explants of the Moroccan olive cultivar Dahbia

Mazri, Mouaad Amine; Belkoura, I.; Pliego‐Alfaro, Fernando; Belkoura, M.

doi:10.1016/j.scienta.2013.05.002

Cited by 27 publications

(23 citation statements)

References 34 publications

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“…In a subsequent experiment, the protocol of Mazri et al (2013) was assayed using the four genotypes. No noticeable changes were observed after 4 days in liquid induction medium (1/2 MS 30 µM TDZ-0.54 µM NAA) ( Figure 1A ).…”

Section: Resultsmentioning

confidence: 99%

“…In a different experiment, the protocol of Mazri et al (2013) for cultivated olive was evaluated, i.e., explants of the four genotypes (Ac-18, OutVert, StopVert and Ac-15) were cultured on 5 ml liquid induction medium composed by 1/2 MS mineral elements, MS vitamins, 100 mg/L myo-inositol, 30 µM TDZ and 0.54 µM NAA, in septate Petri dishes for 4 days, over an orbital shaker (platform size 41 × 41 cm, New Brunswick Scientific, Edison, NJ), at 80 rpm. Afterwards, explants were cultured on solid 1/2 MS medium without growth regulators for two recultures of 4 weeks each.…”

Section: Methodsmentioning

confidence: 99%

“…Canino and Moraiolo. Other authors used leaf and petioles isolated from in vitro shoots of cultivars Dahbia (Mazri et al, 2013) and Picual (Toufik et al, 2014). In wild olive, self-rooted plants in greenhouse were an adequate source of explants; however, plant regeneration was not reported (Capelo et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Plant Regeneration via Somatic Embryogenesis in Mature Wild Olive Genotypes Resistant to the Defoliating Pathotype of Verticillium dahliae

et al. 2019

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Regeneration capacity, via somatic embryogenesis, of four wild olive genotypes differing in their response to defoliating Verticillium dahliae (resistant genotypes StopVert, OutVert, Ac-18 and the susceptible one, Ac-15) has been evaluated. To induce somatic embryogenesis, methodologies previously used in wild or cultivated olive were used. Results revealed the importance of genotype, explant type, and hormonal balance in the induction process. Use of apical buds obtained from micropropagated shoots following a methodology used in cultivated olive (4 days induction in liquid 1/2 MS medium supplemented with 30 µM TDZ–0.54 µM NAA, followed by 8 weeks in basal 1/2 MS medium) was adequate to obtain somatic embryos in two genotypes, StopVert and Ac-18, with a 5.0 and 2.5% induction rates, respectively; however, no embryogenic response was observed in the other two genotypes. Embryogenic cultures were transferred to basal ECO medium supplemented with 0.5 µM 2iP, 0.44 µM BA, and 0.25 µM indole-3-butyric acid (IBA) for further proliferation. Somatic embryos from StopVert were maturated and germinated achieving a 35.4% conversion rate. An analysis of genetic stability on StopVert, using Simple Sequence Repeats (SSRs) and Random Amplified Polymorphic DNA (RAPDs) markers, was carried out in embryogenic callus, plants regenerated from this callus and two controls, micropropagated shoots used as explant source, and the original mother plant. Polymorphism was only observed in the banding pattern generated by RAPDs in 1 of the 10 callus samples evaluated, resulting in a variation rate of 0.07%. This is the first time in which plants have been regenerated via somatic embryogenesis in wild olive.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Plant Regeneration via Somatic Embryogenesis in Mature Wild Olive Genotypes Resistant to the Defoliating Pathotype of Verticillium dahliae

et al. 2019

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“…Recalcitrance is associated with the use of adult plant tissues with exceptions known for cvs. 'Canino', 'Moraiolo', 'Chetoui','Dahbia', and 'Picual', for which were used petioles/leaf tissues taken from in vitro growing plants, to successfully induce SE and further embryos differentiation [34][35][36][37]. Embryos conversion, known as a key step in an efficient SE protocol, was limited to cv.…”

Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis from Mature Embryos of Olea europaea L. cv. ‘Galega Vulgar’ and Long-Term Management of Calli Morphogenic Capacity

Pires

Cardoso

Ribeiro

et al. 2020

Plants

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Several olive cultivars, characterized by high-quality olive oil show agronomical issues such as excessive vigor, high susceptibility to biotic and abiotic stresses, and low propagation ability. They are strong candidates for breeding based on new technologies to improve their performance in a short period of time. For this reason, the first step is developing efficient somatic embryogenesis (SE) protocols. Somatic embryogenesis in olive is highly genotype-dependent for both adult tissues and mature embryos as initial explants, requiring the development of specific protocols for each genotype. Trials using cotyledons and radicles as initial explants, isolated from ripe seeds from the Portuguese olive cv. ‘Galega vulgar’, gave more than 95% calli development. Radicles proved to be the most responsive tissue for SE induction, with an average of 2 embryos per callus after callus transfer to expression medium, and 14 embryos per callus after subculture on the olive cyclic embryogenesis medium (ECO). Embryogenic competence could be recovered after several subcultures on ECO medium that maintained cyclic embryogenesis for an indeterminate period of time. Embryo conversion and plant acclimatization were also attained with high success rates. Media management for cyclic embryogenesis maintenance is of general importance for SE protocols in any olive genotype. Somatic embryogenesis was thus attained for the first time in embryo-derived explants of cv. ‘Galega vulgar’.

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“…Nevertheless, application of 2,4-D to induce mangosteen somatic embryos resulted scarcely regenerated calli (Te-chato 1998; Rohani et al 2012). Besides auxin, thidiazuron (TDZ) and benzyladenine (BA) were used for inducing somatic embryogenesis on date palm (Aslam et al 2011), Camellia nitidissima (Lu et al 2013), olive (Mazri et al 2013) and chestnut (Sezgin and Dumanoglu 2014). The combination of 0.7 mg l -1 TDZ and 0.7 mg l -1 BA on mangosteen culture produced 40% globular somatic embryos (Elviana et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Sources of Plant Growth Regulator on the Induction and Development of Mangosteen Somatic Embryos

Joni¹,

Prihatini²,

Efendi³

et al. 2017

Indones. J. Agric. Sci.

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Somatic embryogenesis is a technique for regenerating embryos derived from somatic cells of various plant species. This technique along with the utilization of plant growth regulator (PGR) might benefit for mass propagation and improvement of plant species through biotechnological tools. The study aimed to determine the effect of different plant growth regulators, namely 6-benzyladenine (BA) and thidiazuron (TDZ) on the embryogenic callus induction as well as casein hydrolysate and malt extract on the somatic embryo development of mangosteen. The explants used were in vitro young stems of mangosteen clone Leuwiliang. This study consisted of two experiments, namely induction of embryogenic callus and formation of somatic embryo. The first experiment was arranged as factorial in a completely randomized design with BA (0 and 0.7 mg l -1 ) as the first factor and TDZ (0, 0.1, 0.5 and 1.0 mg l -1 ) as the second factor. The second experiment consisted of four treatments, i.e. casein hydrolysate and malt extract at the rate of 500 and 1,000 mg l -1 . The results showed that the best medium for embryogenic callus induction was MS supplemented with 0.1 mg l -1 TDZ, which resulted semifriable calli. Casein hydrolysate and malt extract could not induce the formation of somatic embryos. After two times subcultures on the same MS medium supplemented with 0.5 mg l -1 TDZ and 0.7 mg l -1 BA, a total of 33.8 somatic embryos per explant was induced. The successful somatic embryogenesis would support mangosteen breeding and in vitro mass propagation program.

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Somatic embryogenesis from leaf and petiole explants of the Moroccan olive cultivar Dahbia

Cited by 27 publications

References 34 publications

Plant Regeneration via Somatic Embryogenesis in Mature Wild Olive Genotypes Resistant to the Defoliating Pathotype of Verticillium dahliae

Plant Regeneration via Somatic Embryogenesis in Mature Wild Olive Genotypes Resistant to the Defoliating Pathotype of Verticillium dahliae

Somatic Embryogenesis from Mature Embryos of Olea europaea L. cv. ‘Galega Vulgar’ and Long-Term Management of Calli Morphogenic Capacity

Effect of Different Sources of Plant Growth Regulator on the Induction and Development of Mangosteen Somatic Embryos

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