Somatic Embryogenesis in Aspidosperma polyneuron Mull. Arg.

Ribas, Luciana Lopes Fortes; Guerra, Miguel Pedro; Zanette, Flávio; Kulchetscki, Luiz

doi:10.1007/978-94-017-3030-3_17

Cited by 4 publications

(2 citation statements)

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“…Histological observations revealed that plant cells about to undergo in vitro developmental processes have elevated starch contents (Mangat et al 1990). Ribas et al (2000) observed starch accumulation in embryogenic cells of Aspidosperma polyneuron. Jensen (1963) observed an abundance of starch in the zygote during the early stages of embryo development in cotton.…”

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confidence: 86%

Relationship between starch accumulation and organ development at the different growth stages of callus in Kihada (Phellodendron amurense Rupr.)

Karim

Yokota

Azad

et al. 2006

Plant Biotechnology

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Microscopic observations of Kihada (Phellodendron amurense) callus revealed a strong correlation between starch accumulation and organ development. The starch accumulation started before any observable organ development and was prominent in cells which ultimately gave rise to organ primordia. A large amount of starch accumulation was observed in the three-or four-celled proembryo-like structure (PLS) organization of callus cultured on MS media supplemented with BAP plus 2,4-D. Two-celled PLS showed low performance for accumulation of starch. No starch accumulation was observed in globular and heart-shaped embryo-like structure ELS organization. During shoot primordium development, a decrease in the starch content of the cultured tissues was found, indicating the utilization of the glucan in the organogenic process. Starch granules disappeared with the development of the meristematic dome and leaf primordium and also started to disappear gradually with the development of cell walls and the tracheary elements.Key words: Callus, kihada, organ development, organogenesis, starch accumulation. Tissue Culture Note Copyright © 2006 The Japanese Society for Plant Cell and Molecular BiologyAbbreviations: BAP, 6-benzylaminopurine; 2,4-D, 2,4-dichlorophenoxyacetic acid; ELS, embryo-like structure; IBA, indole-3-butyric acid; MS, Murashige and Skoog; NAA, a-naphthaleneacetic acid; PGR, plant growth regulator; PLS, proembryo-like structure. This article can be found at http://www.jspcmb.jp/ cell to develop shoots or roots. The degradation products of starch are utilized for the elongation of the cells, possible in the buildup of new cell wall materials. If starch was absent in the cell, callus failed to initiate any organ.Following this view, we attempted to examine the relationship between starch accumulation and organ development at different growth stages of callus in Kihada. We can get a lot of callus within a short time by subculture using proper plant growth regulator. Before investigating the relationships between the biosynthesis of medicinal compounds and organ development, the present study examined the relationships between starch accumulation and organ development in Kihada.There are some reports concerning the in vitro culture of Kihada related to mass propagation by axillary bud/callus culture (Azad et al. 2004(Azad et al. , 2005. To our knowledge, this is the first report on the observation of a relation between starch accumulation and organ development from hypocotyl-derived callus in Kihada.Fruits of Kihada were obtained from a 50-year-old tree growing at the Medicinal Plant Garden of Kumamoto University, Japan. Flesh was removed from fruits, and seeds were recovered. They were washed with detergentcontaining tap water for 15 min and then rinsed thoroughly with running tap water for 20 min. They were surface-sterilized with 70% ethanol for 3 min and then with 3% (v/v) sodium hypochlorite aqueous solution (Wako Pure Chem. Ind. Ltd., Japan) for 20 min. The surface-sterilized seeds were then washed with ...

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confidence: 86%

Relationship between starch accumulation and organ development at the different growth stages of callus in Kihada (Phellodendron amurense Rupr.)

Karim

Yokota

Azad

et al. 2006

Plant Biotechnology

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“…A organogênese e a embriogênese somática correspondem às rotas morfogênicas que podem ser induzidas para a clonagem de materiais genéticos de interesse (RIBAS et al, 2000). A organogênese tem sido descrita para diversas espécies de Eucalyptus (BARRUETO CID et al, 1999;DIBAX et al, 2005;NUGENT et al, 2001a;MULLINS et al, 1997;MURALIDHARAN;MASCARENHAS, 1987) bem como a embriogênese somática (NUGENT et al, 2001b;PINTO et al, 2008 MINOCHA, 1990;AZMI et al, 1997;BANDYOPADHYAY et al, 1999), ápices (NICCOL et al, 1994; segmentos nodais (GIRIJASHANKAR, 2012;BRONDANI et al, 2012b …”

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