Somatic embryogenesis in Buchanania lanzan spreng

Sharma, Parul; Koche, Vijaya; Quraishi, Afaque; Mishra, Sunita

doi:10.1079/ivp2005680

Cited by 10 publications

(9 citation statements)

References 6 publications

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“…The highest frequency (88.4%) of somatic embryos on MB medium was obtained with 13.57 μM 2,4-D (Table 3, medium 3). It was reported that somatic embryogenesis needed a high concentration of auxins and cytokinins in early stages of embryo induction and development (Sharma et al 2005). However, in this experiment, there was less embryogenesis on 2,4-Dcontaining medium with KT than without.…”

Section: Resultscontrasting

confidence: 55%

“…However, sometimes they were not effective in embryogenesis inductions (Sharma et al 2005). The balance between auxins and cytokinins can also affect somatic embryogenesis (Ahloowalia 1987;Eapen and George 1993;Fulzele and Satdeve 2003;Sharma et al 2005). We have shown that embryogenic calluses proliferation was affected by the type of explants.…”

Section: Resultsmentioning

confidence: 89%

“…Cytokinins, especially thidiazuron, could also induce somatic embryogenesis and multiple shoots (Gill and Ozias-Akins 1999;Mithila et al 2003;Su et al 2005). However, sometimes they were not effective in embryogenesis inductions (Sharma et al 2005). The balance between auxins and cytokinins can also affect somatic embryogenesis (Ahloowalia 1987;Eapen and George 1993;Fulzele and Satdeve 2003;Sharma et al 2005).…”

Section: Resultsmentioning

confidence: 99%

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High-frequency embryogenesis and regeneration of plants with high content of gentiopicroside from the Chinese medicinal plant Gentiana straminea Maxim.

Cai

Liu

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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Gentiana straminea Maxim. (MahuaQinjiao) is an important Chinese medicinal herb that is rich in secoiridoids. To increase the production of this plant, we have developed an efficient tissue culture system for highfrequency plant regeneration. We have also obtained plants with high secoiridoid monomer production through tissue culture and somatic embryogenesis. We have found that the MB medium is better than the B5 medium for callus induction. Calluses induced from immature seeds are superior to those from hypocotyls or young leaves in regeneration via somatic embryogenesis. We have also demonstrated that 2,4-dichlorophenoxyacetic acid is efficient for both callus induction and embryogenesis, indole-3-acetic acid is suitable for embryogenic callus proliferation, and N 6 -(benzyl)-adenine promotes both embryo development and the accumulation of gentiopicroside in the cultures. Regenerated plants have been selected for high gentiopicroside content. One plant contains 5.82% of gentiopicroside, which is higher than the control plants (1.20-3.73%). The regenerated plants are genetically more stable than the calluses based on both cytological and random amplified polymorphic DNA analyses.

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Section: Resultscontrasting

confidence: 55%

Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

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High-frequency embryogenesis and regeneration of plants with high content of gentiopicroside from the Chinese medicinal plant Gentiana straminea Maxim.

Cai

Liu

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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“…The 2,4-D, an auxin analog used as herbicide has been shown to play signaling role in induction of SE in many plant systems (Nomura and Komamine 1995). In tree species like Dalbergia sissoo (Singh and Chand 2003) and Buchanania lanzan (Sharma et al 2005), 2,4-D in combination with cytokinin (BA/KIN) has induced embryogenic callus that later differentiated into somatic embryos. In the present study, the callus was produced either in the presence of auxin (2,4-D) or auxin and cytokinin (2,4-D:BA) combinations, but the induced callus remained nonembryogenic until it was subcultured to a medium with a cytokinin (BA or 2iP), where the callus became embryogenic and produced somatic embryos.…”

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration in Pterocarpus marsupium Roxb.

Husain

Anis

Shahzad

2010

Trees

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Somatic embryogenesis (SE) has been achieved from hypocotyl-derived callus culture in Pterocarpus marsupium. Ninety percent of hypocotyl explants (excised from 12-day-old in vitro germinated axenic seedlings) produced callus on Murashige and Skoog medium supplemented with 5 lM 2,4-dichlorophenoxyacetic acid and 1 lM a 6-benzyladenine (BA). Induction of SE occurred after transfer of callus clumps (200 ± 20 mg fresh mass) to MS medium supplemented with BA at 2.0 lM, where a maximum of 23.0 ± 0.88 globular stage embryos per callus clump were observed after 4 weeks of culture. Subculturing of these embryos on MS medium supplemented with 0.5 lM BA, 0.1 lM a-naphthalene acetic acid and 10 lM abscisic acid significantly enhanced the maturation of somatic embryos to early cotyledonary stage, where 21.4 ± 0.32 embryos per callus clump were recorded after 4 weeks of culture. Of 30-well developed somatic embryos, 16.6 ± 0.33 germinated and subsequently converted into plantlets on halfstrength MS medium supplemented with 1.0 lM BA. The morphologically normal plantlets with well-developed roots were first transferred to 1/4-liquid MS medium for 48 h and then to pots containing autoclaved soilrite and acclimatized in a culture room. Thereafter, they were transferred to a greenhouse, where 60% of them survived.

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“…Complete plantlet regeneration has been reported in some medicinal trees such as Azadirachta indica (Quraishi et al 2004a, b), Acacia catechu (Sahini and Gupta 2002) and Terminalia arjuna (Pandey and Jaiswal 2002). Similarly, reports on micropropagation of Aegle marmelos (Ajithkumar and Seeni 1998), Calophyllum inophyllum (Thengane et al 2006), Gmelina arborea (Naik et al 2003), Terminalia chebula (Anjaneyulu et al 2004), Ocotea bullata and Warburgia salutaris (Kowalski and Van Staden 2001), Buchanania lanzan (Sharma et al 2005), Pterocarpus santalinus (Rajeswari and Paliwal 2006) and Simmondsia chinensis (Bashir et al 2007) are also available.…”

Section: Introductionmentioning

confidence: 92%

In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: a medicinal tree

Shukla

Mishra

2008

Trees

Self Cite

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Padar (Stereospermum personatum, family Bignoniaceae) is a well-known medicinal tree. Its complete regeneration occurred through shoot bud culture in vitro. The seeds germinated sequentially on plastic trays and polyethylene bags for 21 days served as explants source. Nodal segments from the seedlings were established on MS medium supplemented with 4.44 lM BA, in which 86.6% nodes showed shoot bud elongation. Then, nodal segments from the developed shoots were cultured on MS medium with several BA concentrations; best shoot multiplication was obtained with 0.44 lM BA. In a second experiment where PVP was added to proliferation medium, nodal segments from developed shoots produced maximum 2.78 shoots per node. The nodal segments showed shoot multiplication up to seventh subculture on. Finally, shoots were rooted on MS medium with 2.46 lM IBA. The plants transferred to net pots containing coco-peat were acclimatized in green house, where more than 80% plants survived and grew normally.

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Somatic embryogenesis in Buchanania lanzan spreng

Cited by 10 publications

References 6 publications

High-frequency embryogenesis and regeneration of plants with high content of gentiopicroside from the Chinese medicinal plant Gentiana straminea Maxim.

High-frequency embryogenesis and regeneration of plants with high content of gentiopicroside from the Chinese medicinal plant Gentiana straminea Maxim.

Somatic embryogenesis and plant regeneration in Pterocarpus marsupium Roxb.

In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: a medicinal tree

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