Somatic Embryogenesis in Buckwheat (Fagopyrum Mill.) and Sorrel (Rumex L.), Polygonaceae

Nešković, Mirjana; Ćulafić, Lj.; Vujičić, Radmila

doi:10.1007/978-3-642-78643-3_33

Cited by 4 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shoot regeneration may also vary depending on the genotype of the donor plant. Differences in shoot regeneration have been found in many buckwheat species (Lachmann and Adachi, 1990;Neskovic et al, 1995). This variation was also found in our experiments.…”

Section: Discussionsupporting

confidence: 84%

“…At the cotyledonary stage, many embryos were small and not very conspicuous. A similar phenomenon was reported by Neskovic et al (1995). However, in our experiments with F. esculentum direct regeneration of shoots was also observed in a few cases.…”

Section: Discussionsupporting

confidence: 82%

“…Modern biotechnology may provide means to address these problems in a novel way (Neskovic et al, 1995). In vitro regeneration of buckwheat has been reported from explants such as hypocotyls (Yamane, 1974;Lachmann and Adachi, 1990); cotyledons (Srejovic and Neskovic, 1981;Miljus-Djukic et al, 1992); immature inflorescence (Takahata, 1988); and anthers (Adachi et al, 1989;Bohanec et al, 1993).…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Plant regeneration from cotyledon tissues of common buckwheat (Fagopyrum esculentum Moench)

Woo

Nair

Adachi

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Plants were regenerated from cotyledon tissue of greenhouse grown seedlings of common buckwheat (Fagopyrum esculentum Moench.). Maximum callus regeneration was induced on Murashige and Skoog (MS) medium containing 2,4-D (2.0 mg l 21 ) and kinetin (KIN) (0.2 mg l 21 ) and either 3 or 6% sucrose. Friable callus was transferred to MS media containing KIN and benzylaminopurine (BAP) at varied concentrations for embryogenic callus induction. The optimum medium for embryogenic callus induction was found to be MS medium supplemented with 0.2 mg l 21 KIN, 2.0 mg l 21 BAP and 3% (w/v) sucrose. Variation of sucrose from 3 to 6% did not show any significant effect on callus induction or embryogenesis. Regeneration of embryonic callus varied from 13 to 32%. Whole plants were obtained at high frequencies when the embryogenic calluses with somatic embryos and organized shoot primordia were transferred to half-strength MS media with 3% sucrose. Regenerated plants after acclimation were transferred to greenhouse conditions, and both vegetative and floral characteristics were observed for variation. This regeneration system may be valuable for genetic transformation and cell selection in common buckwheat.

show abstract

Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Plant regeneration from cotyledon tissues of common buckwheat (Fagopyrum esculentum Moench)

Woo

Nair

Adachi

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

show abstract

“…At the cotyledonary stage, many embryos were very small and inconspicuous. A similar phenomenon was reported by Neskovic et al, (1995) in common buckwheat. However, in our experiments, direct regeneration of shoots also was observed occasionally.…”

Section: Resultssupporting

confidence: 88%

“…Modern biotechnology may provide the means of overcoming these problems in novel ways (Neskovic et al, 1995). The main objective of this research was to develop a rapid and simple method for high efficiency plant regeneration from the hypocotyls of buckwheat.…”

mentioning

confidence: 99%

Plantlet Regeneration via Somatic Embryogenesis from Hypocotyls of Common Buckwheat (Fagopyrum esculentum Moench.)

Kwon¹,

Han²,

Huh³

et al. 2013

Korean Journal of Crop Science

View full text Add to dashboard Cite

Buckwheat sprout is used as vegetable, and also flour for making noodles, and so on. Currently, information about tissue culture in buckwheat is limited and restricted to micro-propagation. We carried out somatic embryogenesis and plant regeneration using hypocotyl segments as explant of the cultivated buckwheat species, Fagopyrum esculentum which differs from existing studies in the growth regulator combinations used. Maximum callus regeneration was induced on MS medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) 2.0 mg · L ). Only 2,4-D did not show any significant effect on callus induction or embryogenesis. Regeneration of embryonic callus varied from 5% to 20%. Whole plants were obtained at high frequencies when the embryogenic calli with somatic embryos and organized shoot primordia were transferred to MS media with 3% sucrose. The main objective of this research was to develop an efficient protocol for plant regeneration for common buckwheat, and to apply in future for genetic transformation.

show abstract

Concepts, Prospects, and Potentiality in Buckwheat (Fagopyrum esculentum Moench): A Research Perspective

Woo

Roy

Kwon

et al. 2016

Molecular Breeding and Nutritional Aspects of Buckwheat

View full text Add to dashboard Cite

Somatic Embryogenesis in Buckwheat (Fagopyrum Mill.) and Sorrel (Rumex L.), Polygonaceae

Cited by 4 publications

References 19 publications

Plant regeneration from cotyledon tissues of common buckwheat (Fagopyrum esculentum Moench)

Plant regeneration from cotyledon tissues of common buckwheat (Fagopyrum esculentum Moench)

Plantlet Regeneration via Somatic Embryogenesis from Hypocotyls of Common Buckwheat (Fagopyrum esculentum Moench.)

Concepts, Prospects, and Potentiality in Buckwheat (Fagopyrum esculentum Moench): A Research Perspective

Contact Info

Product

Resources

About