Somatic embryogenesis in Citrus species

Gosal, S. S.; Gill, M. I. S.; Grewal, H. S.

doi:10.1007/978-94-011-0491-3_1

Cited by 13 publications

(7 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In vitro regeneration in citrus has been obtained for several genotypes, through different in vitro techniques, such as somatic embryogenesis from nucellar callus and protoplast cultures (for a review see Gosal et al 1995) and somatic hybrids (for a review see Grosser et al 2000). More recently, efforts have been made to define efficient protocols for the recovery of plants through organogenesis of citrus genotypes (Peña et al 1995, Cervera et al 1998, García-Luis et al 1999, Almeida et al 2003a and related species (Hassanein andAzooz 2003/4, Hiregoudar et al 2005).…”

Section: Introductionmentioning

confidence: 97%

Histological characterization of in vitro adventitious organogenesis in Citrus sinensis

Almeida¹,

Filho²,

Mendes³

et al. 2006

Biologia plant.

View full text Add to dashboard Cite

The adventitious bud development was induced in epicotyl segments of Valencia sweet orange (Citrus sinensis L. Osbeck). Seeds were cultured in vitro for three weeks in the dark, followed by one week at a 16-h photoperiod. Epicotyl segments were cultured horizontally for the induction of organogenesis in Murashige and Tucker (1969, MT) culture medium supplemented with 1.0 mg dm -3 benzylaminopurine. Samples were observed by light and scanning electron microscopy from day zero to day 25, when buds were well grown. It was shown that the adventitious buds originated directly from the cambial region on the cut ends of the explants.

show abstract

Section: Introductionmentioning

confidence: 97%

Histological characterization of in vitro adventitious organogenesis in Citrus sinensis

Almeida¹,

Filho²,

Mendes³

et al. 2006

Biologia plant.

View full text Add to dashboard Cite

show abstract

“…This may provide an appropriate regeneration system for gene transfer techniques. SE has been reported for a growing list of woody angiosperm and conifer tree species, including Betula (Chalupa 1992), Citrus (Gosal et al 1995), Picea (Chalupa 1985; Hakman and von Arnold 1985), Pinus (Newton et al 1995), etc. It has also been observed in several woody legumes, such as Acacia , Albizzia , Robinia and Cercis (Han and Park 1997).…”

Section: Introductionmentioning

confidence: 99%

Effects of light quality on somatic embryogenesis in Araujia sericifera

Torné

Moysset

Santos

et al. 2001

Physiologia Plantarum

View full text Add to dashboard Cite

The effects of photoperiod, light quality and end-of-day (EOD) phytochrome photoconversion on somatic embryogenesis (SE) of Araujia sericifera petals have been studied. Petals from immature flowers were cultured under 8- and 16-h photoperiods using Gro-lux fluorescent lamps. The photon fluence rate was 90-100 µmol m-2 s-1 and the red (R):far-red (FR) ratio was 98. R, FR, R followed by FR (R-FR) and FR followed by R (FR-R) light treatments were applied for 3 weeks at the end of the photoperiods. In a set of experiments, DL-alpha-difluoromethylarginine (DFMA) or methylglyoxal bis(guanylhydrazone) (MGBG), both inhibitors of polyamine biosynthesis, were added to the culture medium in order to study the involvement of polyamine metabolism. The level of SE was the same in long (LD) and short (SD) days. Thus, the light effect was accomplished after 8 h. All EOD treatments that decreased the Pfr level inhibited SE when applied after SD, but not after LD. The FR-R treatment after LD caused an additional stimulatory effect on SE, even in the presence of polyamine inhibitors. DFMA inhibited SE in both SD and LD, but MGBG did not modify SE in either SD or LD. The R, FR and R-FR treatments did not alter the level of SE when applied after LD in the presence of DFMA or MGBG. However, these treatments decreased SE after SD when the medium contained polyamine inhibitors. Our results suggest that Gro-lux lamps, which produce an extremely high R:FR ratio, promote SE in A. sericifera and a timing response to phytochrome photoconversion during photoperiodic induction. Thus, our data corroborate the involvement of phytochromes and polyamines in SE in A. sericifera, which responded as a light-dominant long-day plant.

show abstract

“…The genus Citrus, grown widely throughout tropical and sub-tropical regions, is of considerable economic importance, containing edible fruit species and, as importantly, germplasms for intrageneric rootstocks (1). In India, for example, Citrus ranks third, after banana and mango, with an estimated production of Ͼ3 ϫ 10 6 metric tonnes per year over an area of Ͼ4 ϫ 10 5 hectares (2).…”

Section: Introductionmentioning

confidence: 99%

Pluronic F-68 Enhanced Shoot Regeneration in a Potentially Novel Citrus Rootstock

Cancino

Gill

Anthony

et al. 2001

Artificial Cells, Blood Substitutes, and Biotechnology

Self Cite

View full text Add to dashboard Cite

The effects have been studied in vitro of the non-ionic, co-polymer surfactant, Pluronic F-68, on shoot regeneration and bud induction in epicotyl and cotyledon explants of Citrus depressa, a potential alternative rootstock to C. jambhiri for commercial Citrus. Supplementation of Murashige and Skoog (1962)-based, agar-solidified shoot regeneration/bud induction (SRBI) medium with 1.0 mg l Ϫ1 6-benzylaminopurine and 0.5% (w/v) Pluronic F-68 significantly (P Ͻ 0.05) increased mean fresh weight by a maximum of 60%, the proportion of explants exhibiting shoot /bud regeneration by 25% and the mean number of shoots per epicotyl explant by 184%, compared to untreated controls. Similarly, 0.5% (w/v) Pluronic F-68 significantly (P Ͻ 0.05) enhanced the mean percentage bud induction (91%) and the number of buds regenerated (Ͼ4-fold) per 317

show abstract

Somatic embryogenesis in Citrus species

Cited by 13 publications

References 43 publications

Histological characterization of in vitro adventitious organogenesis in Citrus sinensis

Histological characterization of in vitro adventitious organogenesis in Citrus sinensis

Effects of light quality on somatic embryogenesis in Araujia sericifera

Pluronic F-68 Enhanced Shoot Regeneration in a Potentially Novel Citrus Rootstock

Contact Info

Product

Resources

About