Somatic Embryogenesis in Cryptomeria japonica D. Don: Gene for Phytosulfokine (PSK) Precursor

Somatic Embryogenesis in Conifers: One Clade to Rule Them All?

“…[118] Megagametophyte EM salts + 10 g L −1 sucrose + 10 µM 2,4-D + 5 µM BAP + 0.5 g L −1 casein + 0.5 g L −1 L-glutamine EM salts + 30 g L −1 maltose + 2 g L −1 AC + 100 µM ABA + amino acids + 150 g L −1 PEG + 3.3 g L −1 gellan gum EM salts + 20 g L −1 sucrose + 1.5 g L −1 L-glutamine + 10 g L −1 salts + 30 g L −1 sucrose + 3 µM 2,4-D + 1 µM BAP MS salts + 30 g L −1 maltose + 175 g L −1 PEG 6000 + 2 g L −1 AC + 100 µM ABA Embryogenic culture induction Somatic embryo development Izuno et al [88] Seed EM salts + 10 g L −1 sucrose + 10 µM 2,4-D + 5 µM BAP+ 0.5 g L −1 casein + 0.5 g L −1 L-glutamine + 3 g L −1 gelrite EM salts + 30 g L −1 sucrose + 3 mM 2,4-D + 1 µM BAP + 1.5 g L −1 L-glutamine + 3 g L −1 −1 gelrite EM salts + 175 g L −1 PEG + 100 µM ABA + 2 g L −1 L-glutamine + 1 g L −1 asparagine + 0.5 g L −1 arginine + 3 0.5 g L −1 L-glutamine + 2,4-D + BAP MS salts + 100 µM ABA + 2 g L −1 AC + 150 g L −1 PEG 4000LP salts + 30 g L −1 sucrose + 5 g L −1 AC + 12.5 g L −Wako 0.5 g L −1 L-glutamine + 2,4-D + BAP MS salts + 100 µM ABA + 2 g L −1 AC + 150 g L −1 PEG 4000 LP salts + 30 g L −1 sucrose + 5 g L −1 AC + 12.5 g L −Wako salts + 10 µM 2,4-D + 5 µM BAP + 10 g L −1 sucrose MS/2 salts + 1-10 µM 2,4-D + 0.3 µM BAP + 0.5 g L −1 L-glutamine + 30 g L −1 sucrose EM salts + 50 g L −1 maltose + 100 µM ABA + 2 g L −1 AC + 150 g L −1 PEG 4000 + amino acids EM/2 salts + 2 g L −1 AC + 10 g L −1 10 µM 2,4-D + 30 g L −1 sucrose MS salts + vitamins + 100 µM ABA + 150 g L −1 PEG 4000 + 30 g L −1 maltose MS salts + 2 g L −1 AC + 20 g L −1 sucrose + 5 g L KCl + 0.5 g L −1 casein + 1 g L −1 L-glutamine + 10 g L −1 sucrose + 10 µM 2,4-D + 5 µM BAP EM salts + 50 g L −1 maltose + 100 g L −1 PEG 4000 + 2 g L −1 AC + 100 µM ABA + EMM amino acids µM 2,4-D + 0/2.2/4.4 µM BAP + 10 g L −1 sucrose + 0.5 g L −1 myo-inositol + 1 g L −1 L-glutamine EM salts + 50 g L −1 maltose + 100 gL −1 PEG 4000 + 0/10/50/100 µM ABA + 0/2 g L −1 AC EM salts + 10 g L −1 sucrose + 2 g L salts + g L −1 sucrose + 0.5 g L −1 casein hydrolysate + 0.4 g L −1 L-glutamine + 5 g L −1 Phytagel + 1 g L −1 AC + 0.5 mg L −1 2,4-D + 0.5 mg L −1 KIN DCR salts + 50 µM ABA + 100 g L −1 PEG 6000 Embryogenic culture induction Somatic embryo development Hu et al[103] …”