Somatic Embryogenesis in Forest Plants

Chorabik, Katarzyna Nawrot

doi:10.5772/36280

Cited by 5 publications

(5 citation statements)

References 71 publications

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“…Somatic embryogenesis has the potential to produce plants through in vitro propagation and has now become a routine protocol for many trees ( Jain et al, 2000 ; Nawrot-Chorabik, 2012 ; Germanà and Lambardi, 2016 ). However, the application of somatic embryogenesis in a wide range of woody plants is limited by genotypic influences, poor germination of somatic embryos, and limited numbers of explants ( Isah, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Application of Somatic Embryogenesis in Woody Plants.

et al. 2016

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Somatic embryogenesis is a developmental process where a plant somatic cell can dedifferentiate to a totipotent embryonic stem cell that has the ability to give rise to an embryo under appropriate conditions. This new embryo can further develop into a whole plant. In woody plants, somatic embryogenesis plays a critical role in clonal propagation and is a powerful tool for synthetic seed production, germplasm conservation, and cryopreservation. A key step in somatic embryogenesis is the transition of cell fate from a somatic cell to embryo cell. Although somatic embryogenesis has already been widely used in a number of woody species, propagating adult woody plants remains difficult. In this review, we focus on molecular mechanisms of somatic embryogenesis and its practical applications in economic woody plants. Furthermore, we propose a strategy to improve the process of somatic embryogenesis using molecular means.

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Section: Discussionmentioning

confidence: 99%

Application of Somatic Embryogenesis in Woody Plants.

et al. 2016

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“…Zygotic embryos of silver fir were used as the primary explants for in vitro callus culture using somatic embryogenesis. Each set was represented by 100 explants from which three lines of embryogenic callus with the highest proliferation coefficient (the ratio between mass gain and the diameter of the callus at intervals between passages onto fresh media) representing three separate genotypes were selected for further examinations (Nawrot-Chorabik 2008, 2012. Genotype G4 originated from Nowy Targ, G5 originated from Skarżysko-Kamienna, and G6 originated from Janów Lubelski.…”

Section: Methodsmentioning

confidence: 99%

Interactions between embryogenic callus of Abies alba and Heterobasidion spp. in dual cultures

Nawrot-Chorabik¹

2014

Biologia plant.

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Interactions between three genotypes of a silver fir (Abies alba Mill.) embryogenic callus and Heterobasidion abietinum, H. parviporum, and H. annosum were examined in dual cultures. The aim of this study was to determine whether dual cultures can be used to evaluate the degree of fungal virulence at an embryogenic level, and whether different genotypes of a callus show different susceptibility. The dual cultures were performed on Schenk and Hildebrandt medium. Mycelial growth of H. parviporum and H. annosum was significantly stimulated in the presence of the callus but was not directional in nature. The embryogenic callus died between six and nine days after being colonized by H. parviporum suggesting that this was the most virulent species. By contrast, the callus remained healthy for up to 50 d after colonization with H. abietinum suggesting that this was the least virulent species. The callus of the A. alba genotype which originated in the mountain region of Poland remained healthy and alive for a significantly longer period than the other two genotypes in the dual cultures with all three Heterobasidion species even though overgrown by mycelium suggesting that the mountain genotype had the strongest defence response to Heterobasidion infection.

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“…To achieve this objective, an optimal method for obtaining callus (e.g. somatic embryogenesis) should be selected, disinfection of explants should be developed and media together with growth regulators, concentration of sugars and other components necessary for the proper growth of callus should be selected individually for each species [18].…”

Section: Stages Of Dual Culturesmentioning

confidence: 99%

The Use of Interactions in Dual Cultures in vitro to Evaluate the Pathogenicity of Fungi and Susceptibility of Host Plant Genotypes

Nawrot-Chorabik¹

2013

Environmental Biotechnology - New Approaches and Prospective Applications

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Somatic Embryogenesis in Forest Plants

Cited by 5 publications

References 71 publications

Application of Somatic Embryogenesis in Woody Plants.

Application of Somatic Embryogenesis in Woody Plants.

Interactions between embryogenic callus of Abies alba and Heterobasidion spp. in dual cultures

The Use of Interactions in Dual Cultures in vitro to Evaluate the Pathogenicity of Fungi and Susceptibility of Host Plant Genotypes

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