Somatic Embryogenesis in Maritime Pine (Pinus pinaster Ait.)

Harvengt, Luc

doi:10.1007/1-4020-2985-3_10

Cited by 15 publications

(10 citation statements)

References 12 publications

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“…Presently, the number of embryogenic cell lines that are recalcitrant to current maturation treatment is high in maritime pine (Miguel et al 2004;AFOCEL, unpublished results). Moreover, excessive proliferation of embryogenic tissue (from either recalcitrant or responsive lines) during maturation treatment appeared as the main impediment to enter late embryogeny in maritime pine (Lelu et al 1999;Harvengt 2005). Similarly, over-proliferation of embryogenic tissue was described during Abies alba maturation step.…”

Section: Introductionmentioning

confidence: 91%

Long-term subculture randomly affects morphology and subsequent maturation of early somatic embryos in maritime pine

Breton¹,

Harvengt²,

Trontin³

et al. 2006

Plant Cell Tiss Organ Cult

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Somatic embryogenesis is affected by highly variable maturation yields in Pinus pinaster. Origins of this variability were investigated by testing effects of spatial and temporal division of initial embryogenic tissue into independently cultivated pieces. One embryogenic cell-line was proliferated to obtain six embryonal-suspensor masses (ESM) treated as six sub-lines within a single dish. After proliferation to reach 2, 4 and 8 dishes (12, 24, 48 ESM), the 48 ESM (six sub-lines) were maintained by weekly subculture (34 weeks). ESM observations and maturation tests were regularly performed during the amplification and maintenance periods. Relations between maturation yields as well as cotylfedonary embryo length and immature embryo morphology were analyzed. ESM yielded variable results, independently from culture spatial (dish) and temporal (sub-line) subdivision. Maturation yields and length of regenerated embryos globally decreased as a function of subculture number. Concomitantly, morphological degradation of immature embryos occurred, indicating a global loss of embryogenic ability. Maturation variability probably results from immature embryos heterogeneity, which could be increased by manipulations during subculture. This is the first time that this evolution along time in culture is precisely described in conifers somatic embryos. Keywords Maturation AE Pinus pinaster AE Somatic embryogenesis AE Subculture AE Culture ageing Abbreviations ESM embryonal-suspensor masses f.wt fresh weight CE cotyledonary somatic embryos AE abnormally developed embryos PC precotyledonary and early cotyledonary embryos Electronic Supplementary Material Supplementary material is available to authorised users in the online version of this article at http://dx.

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Section: Introductionmentioning

confidence: 91%

Long-term subculture randomly affects morphology and subsequent maturation of early somatic embryos in maritime pine

Breton¹,

Harvengt²,

Trontin³

et al. 2006

Plant Cell Tiss Organ Cult

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“…1D), and embryogenic tissue was subsequently initiated from the extruded embryo ( Fig 1E). Previously, these tissues have demonstrated that they are capable of producing plants (Park et al 1998;Klimaszewska et al 2001;Lelu et al 1999;Harvengt 2005) and have been successfully cryopreserved.…”

Section: Initiation Of Embryogenic Tissuementioning

confidence: 99%

Initiation of somatic embryogenesis in Pinus banksiana, P. strobus, P. pinaster, and P. sylvestris at three laboratories in Canada and France

Park

Lelu‐Walter²,

Harvengt³

et al. 2006

Plant Cell Tiss Organ Cult

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During 2002During -2004, three laboratories in Canada and France collaborated to improve initiation of somatic embryogenesis (SE) in jack pine (Pinus banksiana Lamb.), eastern white pine (P. strobus L.), maritime pine (P. pinaster Ait.), and Scots pine (P. sylvestris L.), giving particular attention to the effects of (1) N-(2-chloro-4-pyridyl)-N¢-phenylurea (CPPU) versus various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA), (2) differences in basal nutrient media, i.e., macro-and microelements, and (3) gelling agent concentration. The work was carried out separately at each laboratory, but the details of media compositions were shared and tested on their respective species. Results indicate that the developmental stage of the zygotic embryo (ZE) and genotype effects had a large influence on SE initiation, and that genetic effects were consistent over time. Different species responded differently to PGR types and concentration, basal nutrient media, trace elements, and their combinations. Currently, our best initiation rates based on a selected group of genotypes, optimal development stage of ZE, and medium are 3.9% for jack pine, 54.6% for eastern white pine, 76.2% for maritime pine, and 19.7% for Scots pine.

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“…Pinus pinaster (Aiton) seeds (obtained in CENASEF, Centro Nacional das Sementes Florestais, Portugal) were sterilized with sodium hypochloride 5% and two drops of tween® 80 under agitation for 15 min (Harvengt, 2005). After washes with sterilized water, the seeds were poured in ethanol 80% for 15 min, washed again with sterilized water, and finally poured in test tubes (3 cm diameter) with agar:water 0.8%.…”

Section: In Vitro Production Of Mycelia and Germination Of Pinus Pinamentioning

confidence: 99%

Antioxidants in Pinus pinaster roots and mycorrhizal fungi during the early steps of symbiosis

Carocho

Ferreira

Barros

et al. 2012

Industrial Crops and Products

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Ectomycorrhizal symbiosis between fungi mycelia and the roots of some plants could have important effects in the levels of antioxidants of both partners. In the present work, the effects of co-culture period in the antioxidant properties and antioxidants (phenolics, tocopherols and sugars) production during early steps of in vitro mycorrhization (Pinus pinaster-Pisolithus arhizus and Pinus pinaster-Paxillus involutus) were evaluated. The studied parameters were determined in each culture element (root, mycelium and medium) in order to understand the response of each partner to the symbiotic association. P. arhizus proved to be more compatible with P. pinaster than P.involutus, since the antioxidant activity in the latter species increased from 48 to 72 h, while P. arhizus antioxidant properties decreased at the same period (indicative of less oxidative stress). Despite P. involutus proved to be less suitable to be included in forestation programs using mycorrhization processes, it revealed a higher potential for bioactive compounds production in the early steps of symbiosis. A maximal value of phenolics content was obtained after the first 6 h.

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Somatic Embryogenesis in Maritime Pine (Pinus pinaster Ait.)

Cited by 15 publications

References 12 publications

Long-term subculture randomly affects morphology and subsequent maturation of early somatic embryos in maritime pine

Long-term subculture randomly affects morphology and subsequent maturation of early somatic embryos in maritime pine

Initiation of somatic embryogenesis in Pinus banksiana, P. strobus, P. pinaster, and P. sylvestris at three laboratories in Canada and France

Antioxidants in Pinus pinaster roots and mycorrhizal fungi during the early steps of symbiosis

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