Somatic Embryogenesis in Olive (Olea europaea L.)

Rugini, Eddo; Pezza, A.; Muganu, Massimo; Caricato, G.

doi:10.1007/978-3-662-03091-2_27

Cited by 16 publications

(11 citation statements)

References 12 publications

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“…Dolce Agogia, Leccino, Frantoio, and Moraiolo when the fruits were harvested at around 75 days after full bloom, while before or after this period a drastic reduction of frequency with high callus production was observed. The high embryogenic capacity could be maintained for at least 2 months, by storing at 14-15 • C the two and a half-month-old fruits, in which the small embryos continued to develop without losing their embryogenic capacity (Rugini, 1995). These results were confirmed by Leva et al (1995) in cvs.…”

Section: Somatic Embryogenesissupporting

confidence: 78%

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Olive

Rugini

Gutiérrez-Pesce

Muleo

2008

Compendium of Transgenic Crop Plants

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Biochemical and molecular procedures have been developed for olive genotyping, for taxonomy between cultivated and wild olives, for phylogenetic studies of cultivars, and for identification of usable markers linked to the most important agronomic traits, such as size of the tree, flowering induction, apical dominance, productivity, self‐fertility, quantity and quality of oil and secondary products of fruit, biotic and abiotic resistance. These characters could be introgressed into cultivars by conventional breeding or through transgeny. Successful results by classical breeding are difficult to obtain due to the different flower fertility and the long juvenile period of the progenies. The low efficiency of modern genetic improvement techniques suggests that genetic transformation techniques could speed up the development of new genotypes; however, the development of efficient regeneration methods from mature tissue is the limiting factor for applying this technology, in olive. Transgenic olive plants with modified growth habit and putative induced disease resistance are under filed conditions to test their phenotype. Other attempts of genetic improvement such as clonal selection, mutation through γ‐irradiation, protoplast technology, haploid, changes in ploidy level, somaclonal variation, and somatic hybridization are discussed and reviewed.

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Section: Somatic Embryogenesissupporting

confidence: 78%

“…Koroneiki, in Moroccan genotypes (Abousalim et al, 2006), and cv. S. Agostino (Rugini and Tarini, 1986) and Nabali (Shibli et al, 2001). developed a novel strategy named as "double regeneration system" that allowed somatic embryogenesis in two cultivars Canino and Moraiolo.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Olive

Rugini

Gutiérrez-Pesce

Muleo

2008

Compendium of Transgenic Crop Plants

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“…The Romans extended its cultivation from the Greek islands throughout the Mediterranean Basin, mainly along the African and European coasts but also near the great alpine lakes, such as Lakes Iseo, Como, Lugano and Garda, where the large volumes of water ameliorate the climate (Cipriani et al 2002). Currently, 97% of the world production of this economically very important species comes from the Mediterranean Basin (Rugini 1995). Hence, many efforts have been done in the last decades to propagate this commercially valuable species by in vitro methods.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability analyses of the somatic embryogenesis induction process in Olea spp. using nuclear microsatellites

Lopes

Capelo

Brito

et al. 2008

Trees

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The crop species Olea europaea L. (olive tree) is of great economic importance in the Mediterranean region. Hence, many efforts have been done in the last decades to propagate this commercially valuable species by in vitro methods. On the other hand, the lesser known Olea maderensis (Lowe) Rivas Mart. & Del Arco which is a native species of the Madeira Archipelago has only been the subject of micropropagation from nodal stem cuttings. Therefore, in this work we analysed the stability of ten nuclear simple sequence repeat (SSR) markers at successive stages of the somatic embryogenesis process in two adult trees belonging to these two species from the Madeira Archipelago. For the induction of somatic embryogenesis, petiole and leaf explants were cultivated on solid Murashige and Skoog medium (MS) with 12.25 lM indole-3-butyric acid (IBA) and 4.56 lM of zeatin, in the dark. After 3 months, different callus tissues (non-embryogenic, pre-embryogenic and embryogenic) developed from leaf explants and petioles were later transferred to MS medium without growth regulators in the dark. All ten SSR markers were able to distinguish between species. However, no mutations were found at the SSR loci at any of the successive developmental stages from PEMs (pre-embryogenic masses) to somatic embryos. This genetic uniformity was observed within material derived from each genotype/species and its respective donor plant. Therefore, we conclude that the genomic integrities of both O. europaea and O. maderensis were maintained throughout the stages of the embryogenic processes in study suggesting the absence of somaclonal variation.

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“…Olive tree (Olea europaea L.) is a woody fruit crop widely distributed in the Mediterranean regions and comprises several economically important cultivars and wild olive genotypes. The Mediterranean basin provides ideal conditions for olive growing, and supplies more than 97% of the world production of olive (Rugini 1995). Despite of great economical importance, little attention was given to the cytogenetics of this species (2n = 46 chromosomes) with most of the studies concerning the genetic diversity of this species using molecular markers (e.g.…”

Section: Introductionmentioning

confidence: 99%

Nuclear DNA content estimations in wild olive (Olea europaea L. ssp. europaea var. sylvestris Brot.) and Portuguese cultivars of O. europaea using flow cytometry

Loureiro

Rodriguez

Costa

et al. 2006

Genet Resour Crop Evol

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Olive tree (Olea europaea L.) is an economically important woody fruit crop widely distributed in the Mediterranean regions. In this work the genome size of six Portuguese cultivars of olive (O. europaea ssp. europaea var. europaea) and wild olive (O. europaea spp. europaea var. sylvestris) was estimated for the first time. The nuclear DNA content of O. europaea cultivars ranged between 2.90 ± 0.020 pg/2C and 3.07 ± 0.018 pg/2C and the genome size of wild olive was estimated as 3.19 ± 0.047 pg/2C DNA. These results suggest a low intraspecific variation at least among the studied cultivars and between them and wild olive. This is not in accordance with previous results in some Italian cultivars where high genome size heterogeneity was found. The methodology presented here seems appropriate for genome size estimations within this genus and opens good perspectives for a large screening of estimation of nuclear DNA content among O. europaea cultivars and Olea species that could clarify this issue.

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Somatic Embryogenesis in Olive (Olea europaea L.)

Cited by 16 publications

References 12 publications

Olive

Olive

Genetic variability analyses of the somatic embryogenesis induction process in Olea spp. using nuclear microsatellites

Nuclear DNA content estimations in wild olive (Olea europaea L. ssp. europaea var. sylvestris Brot.) and Portuguese cultivars of O. europaea using flow cytometry

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