Somatic Embryogenesis in Peach Palm Using the Thin Cell Layer Technique: Induction, Morpho-histological Aspects and AFLP Analysis of Somaclonal Variation

Steinmacher, Douglas André; Krohn, Nádia Graciele; Dantas, Adriana Cibele de Mesquita; Stefenon, Valdir Marcos; Clement, Charles R.; Guerra, Miguel Pedro

doi:10.1093/aob/mcm153

Cited by 67 publications

(64 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, studies would be necessary with other explant sources (leaf sheaths and inflorescence) using thin cell layer explants to induce somatic embryogenesis. This technique is considered promising for somatic embryogenesis of palm species, e.g., for Elaeis guinensis (Scherwinski-Pereira et al 2010) and B. gasipaes (Steinmacher et al 2007b). The morphogenetic responses observed in this study were similar to the results reported by Guerra and Handro (1998) and Guerra et al (2000) in somatic embryogenesis of E. edulis.…”

Section: Effect Of Sucrose Concentration On In Vitro Germination Of Isupporting

confidence: 89%

In vitro germination and embryogenic competence acquisition of Euterpe edulis Martius immature zygotic embryos

Saldanha

Martins-Corder

2012

Crop Breed. Appl. Biotechnol.

View full text Add to dashboard Cite

show abstract

Section: Effect Of Sucrose Concentration On In Vitro Germination Of Isupporting

confidence: 89%

In vitro germination and embryogenic competence acquisition of Euterpe edulis Martius immature zygotic embryos

Saldanha

Martins-Corder

2012

Crop Breed. Appl. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Furthermore, TCL provides a simple and efficient system of micropropagation that can be used in the propagation of elite clonal plants ( Monja-Mio & Robert 2013). In addition to A. aculeata, the TCL technique has been used with success in Bactris gasipaes (Steinmacher et al 2007c) and in Elaeis guineensis (Scherwinski-Pereira et al 2010). In both cases, the initial explants were leaf transverse TCLs of in-vitro grown seedlings.…”

Section: Discussionmentioning

confidence: 99%

“…The remaining leaf portion was transversally cut from the base into eight slices about one mm thick (TCLs) (Steinmacher et al 2007c), which were used as explants for somatic embryogenesis (Fig. 1).…”

Section: Plant Materials and In Vitro Germinationmentioning

confidence: 99%

“…Somatic embryogenesis holds promise as a technique for palm tree propagation, especially when other propagation techniques prove too difficult or are unviable (Sané et al 2006;Steinmacher et al 2007c). It can be induced in different types of explants, such as zygotic embryos, inflorescences, shoot tips and immature leaves, and may allow the production of many identical plants (Steinmacher et al 2007 a;.…”

Section: Introductionmentioning

confidence: 99%

“…The TCL culture may induce specific morphogenic routes, such as controlled somatic embryo production (Nhut et al 2003). The technique has proven efficient at propagating many plant species (Silva et al 2007;Silva 2010) species, has been used with peach palm (Bactris gasipaes) by Steinmacher et al (2007c) and oil palm (Elaeis guineensis), with leaf explants (Scherwinski-Pereira et al 2010) and immature inflorescences (Guedes et al 2011). …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

et al. 2015

View full text Add to dashboard Cite

Considering the necessity of Acrocomia aculeata propagation for large-scale production, the aim of this study was to establish a somatic embryogenesis protocol using the thin cell layer (TCL) technique. Aerial parts of in vitro plants were transversally cut at the base into eight TCLs and placed in a culture medium for callus induction. The induction medium was composed of Y3 salts and Morel´s vitamins and supplemented with 150, 300 or 600 μM picloram. After 12 weeks the calli were transferred to a medium supplemented with BAP or 2-iP (12.5 or 25 μM). After 18 weeks, the somatic embryo clusters were transferred to a conversion medium (plant growth regulator-free medium). Primary callus induction rate was higher in the first three TCLs and in media containing 150 or 300 μM picloram. The best maturation results were obtained in medium containing 12.5 μM 2-iP or 12.5 μM BAP. Few somatic embryos converted into plants. The histological analyses showed that callus induction started adjacent to vascular bundles after two days of culture, and somatic embryos arose in the periphery of nodular calli. This study showed that the TCL embryogenesis protocol is promising for in vitro multiplication of A. aculeata.

show abstract

Plant Cell Culture – Present and Future

Dunwell

2010

Plant Cell Culture

View full text Add to dashboard Cite

Plant cell culture has a long history of development and exploitation that has been reviewed many times [1]. There are also several monographs and textbooks that provide detailed discussion on specific techniques and their application [2, 3]. By necessity therefore, the present review is limited in scope and, for the most part, will focus on recent publications. The various sections below each concentrate on a specific aspect of the technology; these are followed by a summary section relating to commercialization. MicropropagationMicropropagation in vitro is well established as a method to propagate [4], preserve and transport germplasm of many species including horticultural [5-7], medicinal [8, 9] and woody [10,11] plants. It also reduces the risk of moving pathogens and insects with the germplasm owing to the inherent pathogen detection capabilities of aseptic cultures. Since this technology is usually limited to the multiplication of pre-existing meristems and does not involve the regeneration of plants from single cells or tissue, it will not be discussed in detail here.

show abstract

Somatic Embryogenesis in Peach Palm Using the Thin Cell Layer Technique: Induction, Morpho-histological Aspects and AFLP Analysis of Somaclonal Variation

Abstract: The present study suggests that the TCL somatic embryogenesis protocol developed is feasible, although it still requires further optimization for in vitro multiplication of peach palm, especially the use of similar explants obtained from adult palm trees.

Cited by 67 publications

References 54 publications

In vitro germination and embryogenic competence acquisition of Euterpe edulis Martius immature zygotic embryos

In vitro germination and embryogenic competence acquisition of Euterpe edulis Martius immature zygotic embryos

Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique

Plant Cell Culture – Present and Future

Contact Info

Product

Resources

About