Somatic embryogenesis of Agave victoria-reginae Moore

Abstract

Plant regeneration through direct somatic embryogenesis of leaf blade explants from in vitro propagated plants of Agave victoria-reginae Moore, is described. Somatic embryogenesis was evident in a 6-week period on agarsolidified MS medium supplemented with L2 vitamins and 2,4-dichlorophenoxyacetic acid (1,4 #M), and germination of somatic embryos was achieved after 8 weeks on half-strength MS medium and 4 weeks on half-strength SH medium, both lacking growth regulators. Hyperhydricity of somatic embryos and plantlets was reduced by the use of vented culture vessel lids during the last 4 weeks on SH medium. Shoot proliferation was obtained, and hyperhydricity was eliminated on a modified MS medium (with NH4NO3 reduced to 5 mM) supplemented with kinetin (4.6 #M) and 1-naphthaleneacetic acid (1.6 #M) and the use of vented culture vessel lids.

Plant growth regulators optimization for maximize shoots number in Agave americana L. by indirect organogenesis

“…Roxb. ex Salm-Dyck (Binh et al 1990), A. parrasana A. Berger (Santacruz-Ruvalcaba et al 1999, A. victoria-reginae T. Moore (Rodríguez-Garay et al 1996, Martínez-Palacios et al 2003, A. sisalana Perrine ex Engelm. (Nikam 1997, Hazra et al 2002, Nikam et al 2003 and A. tequilana F.A.C.…”

Somatic Embryogenesis in Agave spp.

“…16.2). On the other hand, an elegant demonstration of the unicellular origin of the A. tequilana somatic embryos was reported by Rodríguez-Domínguez (2000). In this study, gamma rays were used for the bombardment of highly embryogenic cells causing a mutation of the apical cell that resulted from a first cell division giving rise to an albino plantlet with green radicle.…”

Somatic Embryogenesis in Agave: An Overview

“…Direct SE has been reported only in A. victoriae-reginae (Rodríguez-Garay et al 1996) and A. fourcroydes (Monja-Mio and Robert 2013).…”

“…Tissues obtained from a leaf base have been reported for A. victoriae-reginae (Rodríguez-Garay et al 1996), A. vera-cruz (Tejavathi et al 2007), and A. tequilana (Portillo et al 2007;Rodríguez-Sahagún et al 2011).…”

“…Reducing the concentration of the culture medium to half its ionic strength was used in developing embryos of A. victoriae-reginae (Rodríguez-Garay et al 1996) andA. angustifolia (Arzate-Fernández andMejía-Franco 2011).…”