Somatic embryogenesis of Gentiana genus I. The effect of the preculture treatment and primary explant origin on somatic embryogenesis of Gentiana cruciata (L.), G. pannonica (Scop.), and G. tibetica (King)

Abstract

A b s t r a c tExperiments were carried out on three selected species of Gentiana genus: Gentiana cruciata (L.), G. pannonica (Scop.), and G. tibetica (King). Using MS medium supplemented with 2,4-D and kinetin a somatic embryogenesis system of plant regeneration was developed. Induction and intensity of somatic embryogenesis as the effect of integration of the following factors were studied, specifically: seedling pre-treatment (with and without GA3 treatment), light condition (light versus the dark), and type of explant (root, cotyledon and hypocotyl). Numerous significant differences between studied factors were observed and statistically proved.

In vitro morphogenic response of different explants of Gentiana kurroo Royle from Western Himalayas—an endangered medicinal plant

“…The protocol for direct and indirect regeneration presented in the current study could be useful for large scale multiplication of this medicinally important endangered plant. The present investigation was carried out to explore morphogenic potential of different explants (leaf, petiole and root) of Gentiana kurroo and it was recorded that all the explants responded significantly different from each other on similar combinations of growth regulators (Hosokawa et al 1996 andMikula andRybczynski 2001). Petiole explants performed best of all for callus initiation and subsequently the petiole derived callus also proved best for indirect regeneration of shoots.…”

High embryogenic ability and regeneration from floral axis of Amorphophallus konjac (Araceae)

“…Somatic embryogenesis is a complex method of plant regeneration which can be affected by numerous factors: the genetic background of plant material, the manipulation of primary explants initiating culture and culture conditions [12]. Some studies have reported that the culture medium exerts stronger effect on somatic embryogenesis than other factors [15].…”

“…[11]. It is also an efficient method for clonal propagation of genetically stable regeneration in many species [12]. However, the induction of somatic embryogenesis in A. konjac is rarely reported worldwide.…”

Protoplast fusion in the genus Gentiana: genomic composition and genetic stability of somatic hybrids between Gentiana kurroo Royle and G. cruciata L.

“…For over 20 years, we have studied the morphogenic abilities of various gentian species in vitro, aimed at conserving and broadening their genetic diversity (Mikuła et al 1996(Mikuła et al , 2011Mikuła and Rybczyński 2001;Fiuk and Rybczyński 2008;Tomiczak et al 2016). The results of our experiments revealed the tremendous embryogenic potential possessed by Gentiana kurroo Royle.…”