Somatic embryogenesis of tetraploid plants from internodal segments of a diploid cultivar of Asparagus officinalis L. grown in liquid culture

Odake, Yuki; Udagawa, Akihiro; Saga, Hitoshi; Mii, Masahiro

doi:10.1016/0168-9452(93)90018-u

Cited by 20 publications

(8 citation statements)

References 8 publications

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“…Plants arising via organogenesis were tetraploid, whereas those arising by embryogenesis retained their diploid status. This contradicts the results of Odake et al (1993), who regenerated tetraploid asparagus plants via somatic embryogenesis. However, their source tissue was callus, so this result is not unexpected.…”

Section: Discussioncontrasting

confidence: 49%

Culture and Regeneration of Protoplasts from Shoots of Asparagus Cultures

Chen¹,

Conner²,

Christey³

et al. 1997

International Journal of Plant Sciences

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A reliable culture and regeneration protocol has been established for asparagus (Asparagus oficinalis L.) protoplasts isolated from shoots of in vitro plants. Studies focused on factors to maximize plating efficiency, colony formation, and plant regeneration. The optimized protocol involved the culture of asparagus protoplasts embedded in agarose beads through a series of defined media. Etiolated shoot cultures were a superior source for protoplasts than green shoot cultures, and growing feeder cells were critical for high plating efficiency and colony formation. In the presence of growing asparagus feeder cells, asparagus protoplasts initiated cell divisions within 2 d of plating in agarose with plating efficiencies up to 20%. About 80% of the protoplasts initiating cell divisions developed into colonies of at least 25-30 cells, of which 25% initiated somatic embryos. Complete plants were regenerated from the embryos at a frequency over 20%. Overall, ca. 0.8% of the initially isolated protoplasts regenerated into complete plants with this protocol. Plants regenerated from protoplasts have been transferred to soil and maintained phenotypically normal morphology for over 2 yr. Chromosome counts confirmed the diploid status (2n=20) of plants regenerated by somatic embryogenesis.

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Section: Discussioncontrasting

confidence: 49%

Culture and Regeneration of Protoplasts from Shoots of Asparagus Cultures

Chen¹,

Conner²,

Christey³

et al. 1997

International Journal of Plant Sciences

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“…Regarding stability of embling derived plants, cytogenetic studies have revealed contrasting observations. Odake et al (1993) reported chromosome doubling (from diploid to tetraploid) in 66.7% and 100% emblings of Asparagus oYcinalis L. obtained from Gellan Gum-solidiWed medium and liquid medium, respectively. In contrast, Mamiya et al (2001) reported there to be no ploidy changes during somatic embryogenesis in A. oYcinalis.…”

Section: Cytogenetic Analysis Of Variabilitymentioning

confidence: 97%

Stability of potato (Solanum tuberosum L.) plants regenerated via somatic embryos, axillary bud proliferated shoots, microtubers and true potato seeds: a comparative phenotypic, cytogenetic and molecular assessment

Sharma¹,

Bryan²,

Winfield³

et al. 2007

Planta

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The stability, both genetic and phenotypic, of potato (Solanum tuberosum L.) cultivar Desiree plants derived from alternative propagation methodologies has been compared. Plants obtained through three clonal propagation routes-axillary-bud-proliferation, microtuberisation and a novel somatic embryogenesis system, and through true potato seeds (TPS) produced by selfing were evaluated at three levels: gross phenotype and minituber yield, changes in ploidy (measured by flow cytometry) and by molecular marker analysis [measured using AFLP (amplified fragment length polymorphism)]. The clonally propagated plants exhibited no phenotypic variation while the TPS-derived plants showed obvious phenotypic segregation. Significant differences were observed with respect to minituber yield while average plant height, at the time of harvesting, was not significantly different among plants propagated through four different routes. None of the plant types varied with respect to gross genome constitution as assessed by flow cytometry. However, a very low level of AFLP marker profile variation was seen amongst the somatic embryo (3 out of 451 bands) and microtuber (2 out of 451 bands) derived plants. Intriguingly, only AFLP markers generated using methylation sensitive restriction enzymes were found to show polymorphism. No polymorphism was observed in plants regenerated through axillary-bud-proliferation. The low level of molecular variation observed could be significant on a genome-wide scale, and is discussed in the context of possible methylation changes occurring during the process of somatic embryogenesis.

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“…Polyploid regenerants have been identified from tissue cultures of melon, Cucumis melo L . (Bouabdallah & Branchard, 1986 ;Ezura et al ., 1992a, b ;Fassuliotis & Nelson, 1992), another cucurbitaceous species, as well as asparagus, Asparagus ofcinalis L. (Odake et al ., 1993), beet, Beta vulgaris L . (Hansen et al ., 1994), maize, Zea mays L .…”

Section: Introductionmentioning

confidence: 99%

Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro

1996

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Adventitious shoots were obtained from the diploid watermelon [Citrullus lanatus (Thunb .) Matsum . & Nakai] cultivars Dixielee, Jubilee II, Mickylee, Minilee, and Royal Sweet by culturing excised cotyledons on shoot regeneration medium for six weeks. Tetraploid and diploid regenerants were identified by counting the number of chloroplasts per guard cell pair from leaves of regenerated plants . Cross fertilization of putative tetraploids with diploid pollinators and the production of triploid seed confirmed the efficacy of this approach . The mean number of chloroplasts for tetraploid regenerants was 19 .1 whereas diploids averaged 11 .2 . These values were similar to tetraploid and diploid plants from seed . Ovary diameter, petal, and anther diameter of male flowers, and leaf length by width ratio were also good indicators of plant ploidy . Progeny obtained from self-fertile tetraploids of 'Mickylee' were crossed with various diploid pollinators to produce triploid hybrid seed . All triploid plants from tissue culture-derived tetraploids produced fruit comparable in quality to fruit produced by currently-available triploid hybrids, demonstrating that in vitro tetraploid induction can be used to produce high quality tetraploid plants for use in triploid hybrid seed production .

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Somatic embryogenesis of tetraploid plants from internodal segments of a diploid cultivar of Asparagus officinalis L. grown in liquid culture

Cited by 20 publications

References 8 publications

Culture and Regeneration of Protoplasts from Shoots of Asparagus Cultures

Culture and Regeneration of Protoplasts from Shoots of Asparagus Cultures

Stability of potato (Solanum tuberosum L.) plants regenerated via somatic embryos, axillary bud proliferated shoots, microtubers and true potato seeds: a comparative phenotypic, cytogenetic and molecular assessment

Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro

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