Somatic Hybridization in Ferns

Breznovits, A.

doi:10.1007/978-3-642-57945-5_35

Cited by 2 publications

(1 citation statement)

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“…In the T 0 gametophyte generation, expression of BnBBM altered the normally cordate shape of the gametophyte (Figure S4a); these abnormal shapes varied from convex (Figure S4b) to cylindrical (Figure S4c) to shoot‐like structures (Figure S4d). Callus formation, normally seen in fern gametophytes when grown on hormone‐containing media (Bristow, ; Breznovits, ), was also pronounced at the edge of transgenic gametophytes grown on hormone‐free media (Figure S4e). Additional phenotypes were seen on the shoots at a low penetrance in the T 1 sporophyte generation.…”

Section: Resultsmentioning

confidence: 89%

A fern AINTEGUMENTA gene mirrors BABY BOOM in promoting apogamy in Ceratopteris richardii

et al. 2017

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Asexual reproduction is widespread in land plants, including ferns where 10% of all species are obligate asexuals. In these ferns, apogamous sporophytes are generated directly from gametophytes, bypassing fertilization. In the model fern Ceratopteris richardii, a sexual species, apogamy can be induced by culture on high sugar media. BABY BOOM (BBM) genes in angiosperms are known to promote somatic embryogenesis, which like apogamy produce sporophytes without fertilization. Here, a Brassica napus BBM (BnBBM) was used to investigate genetic similarity between apogamy in ferns and somatic embryogenesis in angiosperms. A C. richardii transcriptome was constructed from which one AINTEGUMENTA-LIKE unigene, CrANT, was identified. Whole mount in situ hybridization showed that CrANT is expressed in sperm and fertilized eggs. Phylogenetic analysis grouped CrANT with other non-seed-plant ANT genes to the euANT clade but in a branch separate from BBM genes. Overexpression of CrANT or BnBBM promotes apogamy in C. richardii without sugar supplement. CrANT knockdown gametophytes responded weakly to sugar for apogamy promotion. Theses results suggest some genetic conservation between apogamy and somatic embryogenesis and that such asexual reproduction may be ancient.

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Section: Resultsmentioning

confidence: 89%