Somatic hybridization in the genus Solanum: S. tuberosum and S. brevidens

Barsby, T. L.; Shepard, James F.; Kemble, R. J.; Wong, R. S. C.

doi:10.1007/bf00270215

Cited by 97 publications

(19 citation statements)

References 15 publications

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“…During the present hybrid selection procedures, elimination of radish chromosomes and cabbage chloroplasts was found to occur. The somatic hybrid plants between sexually incompatible species flowered but non-viable pollen grains were produced (Shepard et al, 1983;Barsby et al, 1984;Fig. 2.…”

Section: Resultsmentioning

confidence: 99%

Transfer of radish (Raphanus sativus L.) chloroplasts into cabbage (Brassica oleracea L.) by protoplast fusion.

Kameya

Kanzaki

Toki

et al. 1989

The Japanese journal of genetics

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Intergeneric hybrid plants were obtained through protoplast fusion between red cabbage (Brassica oleracea L.) and radish (Raphanus sativus L.). Protoplast fusion was carried out by the dextran method using protoplasts from hypocotyls and cotyledons of both cabbage and radish. The selection of somatic hybrids utilized the inactivation of cabbage protoplasts with iodoacetamide and the low cell-division ability of radish protoplasts. Ten plants were regenerated from selected colonies. Two of them developed to the flowering stage and formed male sterile flowers, which showed cytoplasmic inheritance. The plant and leaf morphology, chromosome number, acid phosphatase and peroxidase isozyme patterns, sexual compatibility, and SmaI cleavage patterns of chloroplast DNA indicated that these plants were intergeneric hybrids having the nucleus of cabbage and chloroplasts of radish.

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Section: Resultsmentioning

confidence: 99%

Transfer of radish (Raphanus sativus L.) chloroplasts into cabbage (Brassica oleracea L.) by protoplast fusion.

Kameya

Kanzaki

Toki

et al. 1989

The Japanese journal of genetics

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“…Somatic potato hybrids have been produced by fusion of diploid wild species with tetraploid S. tuberosum (Barsby et al, 1984;Austin et al, 1993;Cardi et al, 1993;) or with dihaploid S. tuberosum lines (Austin et al, 1985;Rokka et al, 1994). Successful application of the fusion technique demands a protocol for plant regeneration from protoplasts.…”

Section: Introductionmentioning

confidence: 99%

Híbridos somáticos obtenidos por fusión de protoplastos entre Solanum tuberosum L. subsp. tuberosum y la especie silvestre Solanum circaeifolium Bitter

et al. 2008

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Con la finalidad de obtener híbridos somáticos interespecíficos, se fusionaron protoplastos de la especie tetraploide Solanum tuberosum y de la especie silvestre diploide Solanum circaeifolium utilizando polietilenglicol. Los productos de fusión fueron cultivados en el medio V-KM suplementado con albúmina de suero bovino. Las primeras divisiones celulares ocurrieron a los 3 a 4 días de cultivo. Después de la formación de colonias se observó una rápida proliferación de callos, a partir de los cuales se regeneraron 19 plantas. El análisis molecular usando RAPD, confirmó que los regenerantes presentaban segmentos de ADN de ambos parentales, sugiriendo su posible naturaleza de híbridos somáticos. Las observaciones del número de cromosomas indicaron que todos los híbridos fueron aneuploides. En condiciones de invernadero, los regenerantes derivados de la fusión de protoplastos, mostraron características morfológicas intermedias entre las líneas parentales. Este estudio muestra la producción de híbridos somáticos de papa con el método de fusión presentado.

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“…However, breeding using the wild species has been limited due to its sexual incompatibility with S. tuberosum caused by the different ploidy level of the genome and endosperm balance number (Oritz & Ehlenfeldt 1992;Cho et al 1997). Therefore, protoplast fusion of the two different species has been attempted to breed new varieties of potato (Binding et al, 1982;Barsby et al, 1984) and somatic hybridization via protoplast fusion provides opportunity to overcome sexual barriers for interspecific gene transfer with late blight resistance in potato breeding program (Eijlander & Stiekema 1994;Zimnoch-Guzowska et al 2003). The S. nigrum (DU_GS8) was collected in Gyeongsan, South Korea, and has been stored at Daegu University, South Korea.…”

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confidence: 99%

The complete chloroplast genome sequence of potato wild relative species,Solanum nigrum

Park

2016

Mitochondrial DNA Part B

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Solanum nigrum is a wild non-tuber-bearing species belonging to Solanaceae family. The complete chloroplast genome of S. nigrum was constituted by de novo assembly using a small amount of whole genome sequencing data. The chloroplast genome of S. nigrum was 155,432 bp in length and consisted of 25,289 bp of a pair of inverted repeats, 18,402 bp of small single copy, and 85,852 bp of large single copy regions. A total of 139 genes were annotated including 96 protein-coding genes, 39 tRNA genes, and four rRNA genes. Maximum likelihood phylogenetic analysis with 25 Solanaceae species revealed that S. nigrum is grouped with S. melongena. ARTICLE HISTORY

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Somatic hybridization in the genus Solanum: S. tuberosum and S. brevidens

Cited by 97 publications

References 15 publications

Transfer of radish (Raphanus sativus L.) chloroplasts into cabbage (Brassica oleracea L.) by protoplast fusion.

Transfer of radish (Raphanus sativus L.) chloroplasts into cabbage (Brassica oleracea L.) by protoplast fusion.

Híbridos somáticos obtenidos por fusión de protoplastos entre Solanum tuberosum L. subsp. tuberosum y la especie silvestre Solanum circaeifolium Bitter

The complete chloroplast genome sequence of potato wild relative species,Solanum nigrum

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