Somatic Transgenesis (Viral Vectors)

Grinevich, Valery; Knobloch‐Bollmann, H. Sophie; Roth, Lena C.; Althammer, Ferdinand; Domanskyi, Andrii; Vinnikov, Ilya A.; Eliava, Marina; Stanifer, Megan L.; Boulant, Steeve

doi:10.1002/9781118760369.ch12

Cited by 10 publications

(5 citation statements)

References 67 publications

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“…Here, we identified, by a combination of latest state of the art viral-vector based (Grinevich et al, 2016a), anatomical, optogenetic, electrophysiological, and behavioral approaches, a small (n ~30) subpopulation of parvOT neurons in the PVN, which projects to magnOT neurons in the SON and to NK1R or OTR-positive WDR neurons in the deep layers of the SC. Functionally, we demonstrated that this network can inhibit spinal pain processing in a dual manner with two distinct time courses.…”

Section: Discussionmentioning

confidence: 99%

A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing

Eliava

Melchior

Knobloch‐Bollmann

et al. 2016

Neuron

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340

344

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SUMMARY Oxytocin (OT) is a neuropeptide elaborated by the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei. Magnocellular OT neurons of these nuclei innervate numerous forebrain regions and release OT into the blood from the posterior pituitary. The PVN also harbors parvocellular OT cells that project to the brainstem and spinal cord, but their function has not been directly assessed. Here, we identified a subset of approximately 30 parvocellular OT neurons, with collateral projections onto magnocellular OT neurons and neurons of deep layers of the spinal cord. Evoked OT release from these OT neurons suppresses nociception and promotes analgesia in an animal model of inflammatory pain. Our findings identify a new population of OT neurons that modulates nociception in a two tier process: (1) directly by release of OT from axons onto sensory spinal cord neurons and inhibiting their activity and (2) indirectly by stimulating OT release from SON neurons into the periphery.

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Section: Discussionmentioning

confidence: 99%

A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing

Eliava

Melchior

Knobloch‐Bollmann

et al. 2016

Neuron

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340

344

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“…CAV2-Cre was purchased from the Institute of Molecular Genetics, Montpellier (diluted to 10 9 genomic copies/µl, 300 nl per SON). Viruses were injected via a glass pipette into the target regions at 150 nl/min using a syringe pump as described in 50 .…”

Section: Stereotactic Injections Of Viral Vectorsmentioning

confidence: 99%

Social touch promotes interfemale communication via activation of parvocellular oxytocin neurons

et al. 2020

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Oxytocin is a great facilitator of social life, but although its effects on socially-relevant brain regions have been extensively studied, oxytocin neuron activity during actual social interactions remains unexplored. The majority of oxytocin neurons are magnocellular neurons, which simultaneously project to the pituitary and forebrain regions involved in social behaviors. Here, we show that a much smaller population of oxytocin neurons, parvocellular neurons that do not project to the pituitary but which synapse onto magnocellular neurons, is preferentially activated by somatosensory stimuli. This activation is transmitted to the larger population of magnocellular neurons, which consequently show coordinated increases in their activity during social interactions between virgin female rats. Selectively activating these parvocellular neurons promotes social motivation, whereas inhibiting them reduces social interactions. Thus, parvocellular oxytocin neurons, receive somatosensory inputs to control social behavior by coordinating the responses of the much larger population of magnocellular oxytocin neurons.

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“…To assess whether OT neurons located in the PVN (PVN OT neurons) have a significant role in the control of energy homeostasis, we targeted these cells for selective ablation in mice using a diphtheria toxin A (DTA)-based genetic approach. Therefore, we stereotaxically injected adult male mice that carry the DTA gene downstream of a LoxP-STOP-LoxP (LSL) with adeno-associated viruses (AAVs) that drive expression of iCre or the fluorescent reporter Venus under an OT promoter 23 , 24 ( Figure 1 A). As expected, iCre-mediated excision of the LSL cassette resulted in prominent induction of cleaved caspase-3 (C-CASP3; an apoptosis marker) in PVN OT neurons 5 days post AAV injection ( Figures 1 B and S1 A–S1C; Video S1 ).…”

Section: Resultsmentioning

confidence: 99%

High-calorie diets uncouple hypothalamic oxytocin neurons from a gut-to-brain satiation pathway via κ-opioid signaling

Gruber,

Lechner,

Murat

et al. 2023

Cell Reports

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Somatic Transgenesis (Viral Vectors)

Cited by 10 publications

References 67 publications

A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing

A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing

Social touch promotes interfemale communication via activation of parvocellular oxytocin neurons

High-calorie diets uncouple hypothalamic oxytocin neurons from a gut-to-brain satiation pathway via κ-opioid signaling

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