Somatodendritic orientation determines tDCS-induced neuromodulation of Purkinje cell activity in awake mice

Sánchez-León, Carlos A.; Campos, Guillermo Sánchez-Garrido; Fernández, Marta Cejudo; Sánchez-López, Álvaro; Medina, Javier F.; Márquez-Ruiz, Javier

doi:10.1101/2023.02.18.529047

Cited by 4 publications

(1 citation statement)

References 65 publications

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“…These effects on membrane polarization are proposed to be paralleled by changes in spontaneous firing rates [101,102]. Cerebellar tDCS can modulate the membrane polarization of Purkinje and glial cells, as well as mossy and climbing fibres [103,104]. However, the consistency and reproducibility of these effects are limited due to the significant variability among participants; this may depend on several factors, including neuron orientation, and may suggest that the general rule of anodal being excitatory and cathodal inhibitory is probably an oversimplification of the physiological mechanisms underlying tDCS [105].…”

Section: Transcranial Direct Current Stimulation (Tdcs)mentioning

confidence: 99%

Cerebellar Non-Invasive Brain Stimulation: A Frontier in Chronic Pain Therapy

Sveva,

Cruciani,

Mancuso

et al. 2024

JPM

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Chronic pain poses a widespread and distressing challenge; it can be resistant to conventional therapies, often having significant side effects. Non-invasive brain stimulation (NIBS) techniques offer promising avenues for the safe and swift modulation of brain excitability. NIBS approaches for chronic pain management targeting the primary motor area have yielded variable outcomes. Recently, the cerebellum has emerged as a pivotal hub in human pain processing; however, the clinical application of cerebellar NIBS in chronic pain treatment remains limited. This review delineates the cerebellum’s role in pain modulation, recent advancements in NIBS for cerebellar activity modulation, and novel biomarkers for assessing cerebellar function in humans. Despite notable progress in NIBS techniques and cerebellar activity assessment, studies targeting cerebellar NIBS for chronic pain treatment are limited in number. Nevertheless, positive outcomes in pain alleviation have been reported with cerebellar anodal transcranial direct current stimulation. Our review underscores the potential for further integration between cerebellar NIBS and non-invasive assessments of cerebellar function to advance chronic pain treatment strategies.

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Section: Transcranial Direct Current Stimulation (Tdcs)mentioning

confidence: 99%

Cerebellar Non-Invasive Brain Stimulation: A Frontier in Chronic Pain Therapy

Sveva,

Cruciani,

Mancuso

et al. 2024

JPM

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Transsynaptic modulation of cerebellar nuclear cells: theta AC-burst stimulation

Kang,

Talesh,

Lang

et al. 2024

J. Neural Eng.

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Objectives. Transcranial alternating current stimulation (tACS) and its variants are being tested in clinical trials for treatment of neurological disorders, and cerebellar tACS (ctACS) in particular has garnered much interest because of the involvement of the cerebellum in these disorders. The main objective of this study was to investigate the frequency tuning curves for the entrainment of the Purkinje cells (PC) and the cerebellar nuclear (CN) cells by their axonal projections. In addition, we aimed to investigate the temporal and steady-state characteristics of the PC-CN transsynaptic modulation under clinically relevant stimulation waveforms. Approach. Experiments were conducted in anesthetized rats with the electrical stimulations applied to the cerebellar cortex while the spiking activity of PC and CN cells were recorded extracellularly. The PC-CN modulation was tested in a wide range of AC frequencies (1-1,000 Hz). Furthermore, high-frequency AC stimulation (40 Hz to 400 Hz) repeated at 4 Hz, that we termed Theta AC-Burst Stimulation (TACBS), was tested for its transient and steady-state responses. Main results. The CN cell firing patterns suggest that the population of projecting PCs that is entrained by the surface stimulation consists of the cells that are entrained in 180° opposite phases to each other. The CN cell spiking activity in general follows the entrainment pattern of the projecting PCs in the transient response. The CN entrainment during the steady-state turns into suppression at high frequencies of the stimulation. The PC responses could be explained with a simple statistical model that suggested that low-frequency (as well as DC) and high-frequency AC modulation may be operating through different neural mechanisms. Significance. High-frequency AC stimulation with a low-frequency envelope can be leveraged to induce CN modulation at theta frequencies. These results may explain some of the clinical findings and provide insight for future clinical trials of ctACS.

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Inkjet-printed transparent electrodes for electrical brain stimulation

Matta,

Reato,

Lombardini

et al. 2024

Preprint

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Electrical stimulation is a powerful tool for investigating and modulating brain activity, as well as for treating neurological disorders. However, understanding the precise effects of electrical stimulation on neural activity has been hindered by limitations in recording neuronal responses near the stimulating electrode, such as stimulation artifacts in electrophysiology or obstruction of the field of view in imaging. In this study, we introduce a novel stimulation device fabricated from conductive polymers that is transparent and therefore compatible with optical imaging techniques. The device is manufactured using a combination of microfabrication and inkjet printing techniques and is flexible, allowing better adherence to the brain’s natural curvature. We characterized the electrical and optical properties of the electrode and evaluated its performance in the brain of an anesthetized mouse. Furthermore, we combined experimental data with a finite-element model of the in-vivo experimental setup to estimate the maximum electric field that the highly transparent device can generate in the mouse brain. Our findings indicate that the device can generate an electric field as high as 300 V/m, demonstrating its potential for studying and manipulating neural activity using a range of electrical stimulation techniques relevant to human applications. Overall, this work presents a promising approach for developing versatile new tools to apply and study electrical brain stimulation.

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Somatodendritic orientation determines tDCS-induced neuromodulation of Purkinje cell activity in awake mice

Cited by 4 publications

References 65 publications

Cerebellar Non-Invasive Brain Stimulation: A Frontier in Chronic Pain Therapy

Cerebellar Non-Invasive Brain Stimulation: A Frontier in Chronic Pain Therapy

Transsynaptic modulation of cerebellar nuclear cells: theta AC-burst stimulation

Inkjet-printed transparent electrodes for electrical brain stimulation

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