Somatostatin-Like Immunoreactivity (SLIR) in Rat Harderian and Meibomian Glands and Glands of Zeis

Lorber, Mortimer

doi:10.1007/978-1-4615-0717-8_10

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…18 It is possible that this receptor may mediate various actions of somatostatin in the meibomian gland, given that this tissue contains somatostatin positive innervation. 19 The meibomian gland secretions also harboured: (a) a mitochondrial matrix protein, cytochrome c, which has a part in apoptosis; (b) a nuclear protein, farnesoid X activated receptor, which modulates cholesterol homeostasis; and (c) a variety of proteins that would invariably be secreted by other tissues, including laminin a-3 chain, lysozyme C precursor and lipocalin 1. 18 20 Laminin a-3 chain is a subunit of laminin-5 and is believed to be involved in cell adhesion, signal transduction, and keratinocyte differentiation.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of human meibomian gland secretions

Tsai

2006

British Journal of Ophthalmology

123

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Background/aim: Human tears contain hundreds of proteins that may exert a significant influence on tear film stability, ocular surface integrity, and visual function. The authors hypothesise that many of these proteins originate from the meibomian gland. This study's aim was to begin to develop the proteomic methodology to permit the testing of their hypothesis. Methods: Meibomian gland secretions were collected from the lower eyelids of adult volunteers and placed in a chloroform-methanol mixture. Samples were partitioned in a biphasic system and non-lipid phase materials were reduced, alkylated, and trypsin digested to obtain peptides for protein identification. This peptide mixture was separated by m-capillary reverse phase chromatography and the effluent examined by nano-electrospray MS and data dependent MS/MS. SEQUEST software was used to identify proteins from the MS/MS spectra. Results: The methodological approach to date has permitted the identification of more than 90 proteins in human meibomian gland secretions. Proteins include the a2-macroglobulin receptor, IgA a chain, farnesoid X activated receptor, interferon regulatory factor 3, lacritin precursor, lactotransferrin, lipocalin 1, lysozyme C precursor, potential phospholipid transporting ATPase IK, seven transmembrane helix receptor (also termed somatostatin receptor type 4), testes development related NYD-SP21 (also termed high affinity IgE receptor b subunit), and TrkC tyrosine kinase. Conclusions: These findings indicate that the meibomian gland secretes a number of proteins into the tear film. It is quite possible that these proteins contribute to the dynamics of the tear film in both health and disease.

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of human meibomian gland secretions

Tsai

2006

British Journal of Ophthalmology

123

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“…This tissue is the only sebaceous gland in the human body that has rich sensory, sympathetic, and parasympathetic innervation, 145 including contact with nerve fibers reactive for acetylcholinesterase, substance P, vasoactive intestinal peptide, dopamine ␤-hydroxylase, nitric oxide synthase, tyrosine hydroxylase, somatostatin, neuropeptide Y (NPY), and CGRP. 39,56,57,59,81,[157][158][159][160][161][162][163][164][165][166][167][168][169] Further, the meibomian gland contains mRNAs for serotonin, adrenergic, CGRP, cholinergic, dopamine, ␥-aminobutyric acid, glutamate, NPY, neurotensin, and somatostatin receptors (Table 3). 81 It is entirely unclear, however, whether neurotransmitters are released in the vicinity of the meibomian gland, act on glandular receptors, or induce a physiological effect.…”

Section: Regulation Of the Meibomian Gland In Health And Diseasementioning

confidence: 99%

The International Workshop on Meibomian Gland Dysfunction: Report of the Subcommittee on Anatomy, Physiology, and Pathophysiology of the Meibomian Gland

Knop

Millar

et al. 2011

Invest. Ophthalmol. Vis. Sci.

877

968

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“…Immunohistochemical studies revealed no expression of CGRP, PACAP and somatostatin in nerve fibres in the SGTE and DGTE of pigs. On the contrary, in GTE of other animal species the presence of the above‐mentioned neuropeptides in nerve fibres were reported (Hiramatsu et al., 1999; Lorber, 2002; Tuskahara & Jacobowitz, 1987;. Interestingly, the presence of PACAP was found in nerve fibres of GTE in chickens (Hiramatsu et al., 1999).…”

Section: Discussionmentioning

confidence: 97%

“…Interestingly, the presence of PACAP was found in nerve fibres of GTE in chickens (Hiramatsu et al., 1999). The presence of CGRP (Tuskahara & Jacobowitz, 1987) and somatostatin (Lorber, 2002; Peinado et al, 1989) have been demonstrated in the nerve fibres of rat GTE (Lorber, 2002; Peinado et al, 1989; Tuskahara & Jacobowitz, 1987). These data indicate the species‐specific differences in the peptidergic innervation of mammalian lacrimal glands (Tuskahara & Jacobowitz, 1987).…”

Section: Discussionmentioning

confidence: 99%

“…Because tear film is known to moisture and nurture the cornea, both glands are believed to participate in protective mechanisms of the eye (Payne, 1994). In recent decades, various research techniques have been applied to fully explain and understand the mechanisms of neuronal regulation of the mammalian accessory lacrimal glands (Hiramatsu et al., 1999; Lorber, 2002). The use of classical anatomical techniques has led to finding that in most mammals the pterygopalatine ganglion (PPG) is a source of postganglionic parasympathetic fibres supplying SGTE as well as DGTE (Gienc & Kuder, 1985; Motosugi et al., 1992; Cuthbertson et al., 1998).…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical study on the expressions of neuropeptides in the superficial and deep gland of the third eyelid of pigs

Księżarczyk

Arciszewski

2021

Anat Histol Embryol

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The superficial gland of the third eyelid (SGTE) and deep gland of the third eyelid (DGTE) are classified as accessory lacrimal glands. The aim of the present study was to immunohistochemically investigate the expression of vasoactive intestinal peptide (VIP), dopamine beta-hydroxylase (DβH), substance P (SP), galanin, neuropeptide Y (NPY), pituitary adenylate cyclase-activating peptide (PACAP), somatostatin and calcitonin gene-related peptide (CGRP) in the porcine SGTE and DGTE. We demonstrated the distribution patterns of VIP, DβH, SP, NPY and galanin in the nerve fibres in the SGTE and DGTE. None of somatostatin-, PACAP-and CGRP-immunoreactive (IR) nerve fibres were found in the SGTE and DGTE. The majority of VIP-and DβH-IR nerves fibres were found near to glandular acini, tubules, secretory ducts and blood vessels in the SGTE and DGTE. VIP-IR nerve fibres were found in external connective tissue in SGTE and DGTE and only in interlobular connective tissue in the SGTE. DβH-IR nerve fibres were found in interlobular and external connective tissue in the DGTE but not in the SGTE. Single galanin-, SP-and NPY-IR nerve fibres were observed in close proximity to acini and tubules in the SGTE and DGTE. Single galanin-, SP-, NPY-IR nerve fibres were found in close proximity to the secretory ducts in the DGTE, however only SP-IR nerve fibres were found near to the secretory ducts in SGTE. In conclusion, our research highlight some aspects of SGTE and DGTE innervation in pigs and may also be a source of basic knowledge for further studies.

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Somatostatin-Like Immunoreactivity (SLIR) in Rat Harderian and Meibomian Glands and Glands of Zeis

Cited by 6 publications

References 29 publications

Proteomic analysis of human meibomian gland secretions

Proteomic analysis of human meibomian gland secretions

The International Workshop on Meibomian Gland Dysfunction: Report of the Subcommittee on Anatomy, Physiology, and Pathophysiology of the Meibomian Gland

Immunohistochemical study on the expressions of neuropeptides in the superficial and deep gland of the third eyelid of pigs

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