Somatostatin receptor gene expression in neuroblastoma

Albers, Anne; O’Dorisio, M. Sue; Balster, Douglas A.; Caprara, Moonkung; Gosh, P.; Chen, Feng; Hoeger, Carl; Rivier, Jean; Wenger, Gail D.; O’Dorisio, Thomas M.; Qualman, Stephen J.

doi:10.1016/s0167-0115(99)00121-4

Cited by 71 publications

(56 citation statements)

References 29 publications

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“…It has been suggested that somatostatin receptor (SSTR) expression is a favorable prognostic factor in human neuroblastoma because high levels of SSTR type 2 positively relate to patient survival (9,10). In previous studies, we have shown that somatostatin inhibits proliferation, ERK 1/2 and Ras activity in SH-SY5Y cells (11,12), and that these cells express all five SSTR subtypes (13).…”

mentioning

confidence: 85%

Anti-migratory and Anti-invasive Effect of Somatostatin in Human Neuroblastoma Cells

Pola¹,

Cattaneo²,

Vicentini

2003

Journal of Biological Chemistry

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Cell motility and invasion are crucial events for the spread of cancer and, consequently, the metastatic process. Platelet-derived growth factor (PDGF) is not only capable of stimulating the proliferation of SH-SY5Y human neuroblastoma cells, but also their migration and invasion through an extracellular matrix barrier. Experiments using wortmannin and PD98059, specific inhibitors of the phosphatidylinositol 3-kinase (PI3-K) and of the mitogen-activated protein kinases (ERK 1 and 2) signaling, respectively, show that the activation of both pathways is required for the PDGF-induced cell motility responses. We have previously shown that somatostatin inhibits cell division and ERK 1/2 and Ras activity in SH-SY5Y cells. We report here that it is also capable of potently and effectively inhibiting their PDGF-stimulated migration and invasion. The inhibitory effect of somatostatin is sensitive to pertussis toxin. Although somatostatin does not affect PI3-K, it inhibits ERK 1/2 and the small G-protein Rac activation and ruffle formation induced by PDGF. These results indicate that somatostatin can be considered an anti-migratory and antiinvasive agent that acts by inhibiting ERK 1/2 signaling and the PI3-K pathway via the inhibition of Rac in SH-SY5Y cells.

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mentioning

confidence: 85%

Anti-migratory and Anti-invasive Effect of Somatostatin in Human Neuroblastoma Cells

Pola¹,

Cattaneo²,

Vicentini

2003

Journal of Biological Chemistry

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“…stPNET Consistently Display Expression of sst 2 The antibody used for our studies has previously been used in stably transfected cells, expressing sst 2 and, in primary neuroblastomas, expressing the receptor (14). We validated this antibody in normal human cerebellum obtained at autopsy.…”

Section: Resultsmentioning

confidence: 99%

“…IHC analysis was performed using a monoclonal antibody to sst 2. The preparation of the antibody and its application in the study of neuroblastomas has been described previously (14). Tumor tissues were fixed in 10% buffered formalin for 24 h, routinely processed, and paraffin-embedded for histology.…”

Section: Methodsmentioning

confidence: 99%

Somatostatin Receptor Subtype 2 Is Expressed by Supratentorial Primitive Neuroectodermal Tumors of Childhood and Can Be Targeted for Somatostatin Receptor Imaging

Frühwald

Rickert²,

O’Dorisio

et al. 2004

Clinical Cancer Research

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Purpose: Although gliomas predominate among central nervous system (CNS) neoplasms in adulthood, embryonal tumors are the most common malignant brain tumors in children. Despite novel treatment approaches, including improved radiotherapy and high-dose chemotherapy, survival rates remain unsatisfactory. The timely diagnosis of residual or recurrent embryonal CNS tumors and thus the earliest possible time point for intervention is often hampered by inaccuracies of conventional imaging techniques. Novel and refined imaging methodologies are urgently needed.Experimental Design: We have previously demonstrated the use of somatostatin receptor imaging (SRI) in the diagnosis of recurrent and residual medulloblastomas. Here, we evaluated somatostatin receptor type 2 (sst 2 ) expression using an antibody in an array of CNS tumors of childhood. Eight high-grade gliomas, 4 atypical teratoid/rhabdoid tumors, 7 supratentorial primitive neuroectodermal tumors (stPNET), 1 medulloepithelioma (ME), and 8 ependymomas were screened. Tumors positive in vitro were additionally analyzed in vivo using SRI.Results: Abundant expression of somatostatin receptor type 2 in stPNET, a ME, and ependymomas warranted in vivo imaging of 7 stPNET, 1 rhabdomyosarcoma, 3 ependymomas, 1 ME, and 1 glioblastoma. Although SRI was positive in 6/7 stPNET, 1 rhabdomyosarcoma, and 1 ME, none of the ependymomas nor the glioblastoma could be imaged using SRI. In selected cases SRI was more sensitive in the detection of relapse than conventional imaging by magnetic resonance imaging and computed tomography.Conclusions: SRI should be considered in the evaluation of residual or recurrent embryonal CNS tumors, especially stPNET. The strengths of SRI lie in the differentiation of reactive tissue changes versus residual or recurrent tumor, the detection of small lesions, and possibly in the distinction of stPNET from gliomas.

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“…Brain tumors and neuroblastoma (NB) might be future applications of 68 Ga-somatostatin analogues in pediatric oncology. NB shows increased metabolism of catecholamines, but it may also overexpress somatostatin receptors, more precisely SSTR types 1 and 2 [15,16]. Recent studies showed that both 18 F-DOPA and 68 Ga-somatostatin analogues are able to detect NB recurrence/metastases with high sensitivity [17][18][19][20].…”

mentioning

confidence: 99%

Could 68Ga-somatostatin analogues be an important alternative to 18F-DOPA PET/CT in pediatrics?

Piccardo

Treglia

2017

Eur J Nucl Med Mol Imaging

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Somatostatin receptor gene expression in neuroblastoma

Cited by 71 publications

References 29 publications

Anti-migratory and Anti-invasive Effect of Somatostatin in Human Neuroblastoma Cells

Anti-migratory and Anti-invasive Effect of Somatostatin in Human Neuroblastoma Cells

Somatostatin Receptor Subtype 2 Is Expressed by Supratentorial Primitive Neuroectodermal Tumors of Childhood and Can Be Targeted for Somatostatin Receptor Imaging

Could 68Ga-somatostatin analogues be an important alternative to 18F-DOPA PET/CT in pediatrics?

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