Some accounts on culture conditions of callus tissues of Solanum laciniatum Ait. for producing solasodine.

Hosoda, Noriyo; Ichikawa, Hitoshi; Yatazawa, Michihiko

doi:10.1271/bbb1961.43.1745

Cited by 11 publications

(2 citation statements)

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“…IAA at 1 fiM concentration was generally stitnulatory for solasodine production in callus. Previously, Hosoda et al (1979) reported a stimulatory effect of IAA (5.4 [iM) and a-naphthaleneacetic acid (5.8 jiAf) on soiasodine content in tissue cultures of S. laciniatum, whereas Heble et al (1971) could not detect such a stimulatory effect of IAA and indolebutyric acid at 5.4 and 10.8 \iM concentrations in S. xanthoearpum. Both workers used undefined organic additives such as yeast extract and coconut water in the culture medium, and therefore, it would be difficult to attribute such effects solely to the growth regulators in the media.…”

Section: Discussionmentioning

confidence: 93%

“…In vitro cultures of many Solanum spp. have been examined for tbe presence of solasodine, with conflicting results (Vagujfalvi et al 1971, Hosoda et al 1979). However, when solasodine was shown to be produced in culture, it was not clear whether the solasodine content between differentiated and non-differentiated cultures differed (Heble et al 1971, Kokate andRadwan 1979).…”

Section: Introductionmentioning

confidence: 99%

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Studies on some factors affecting solasodine contents in tissue cultures of Solanum nigrum

Bhatt

Sussex

1983

Physiologia Plantarum

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Tissue cultures of Solanum nigrum L. were initiated from leaf explants on a solid medium containing inorganic salts [Murashige and Skoog (1962), Physiol. Plant. 15: 473–497], vitamins [Gamborg et al. (1968) Exp. Cell Res. 50:151–158], 3% sucrose and combinations of indoleacetic acid and benzyladenine. Solasodine content was determined in differentiated and undifferentiated (callus) tissues by a colorimetric technique and thin layer chromatography. Indoleacetic acid and sucrose in the medium markedly stimulated the production of solasodine in the tissue cultures. In the cultures grown in darkness the differentiated tissues produced significantly more (anywhere from 1.5 to 10 times) solasodine than the callus in several media. When sucrose concentration was increased to 4, 6 and 10% level in the medium which contained 10 μM benzyladenine as the sole growth regulator, a significant increase of solasodine production in cultures was found.

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Section: Discussionmentioning

confidence: 93%