Some Aspects of the Action of Tylosin on Clostridium Species PA 3679

Abstract

Spores of PA 3679 were derived from vegetative cells made resistant to an initial 4.0 ppm tylosin.Rate of germination of PA 3679 spores, either from the original sensitive stock or from a resistant stock, is not affected by tylosin. Vegetative cells of PA 3679 arising from germination and subsequent outgrowth of the resistant cells are killed by 0.8-0.9 ppm of residual tylosin. This is to he compared with 0.05-0.25 ppm initial tylosin required to kill sensitive cells. The residual level of tylosin in sterile spore-recovery media is dependent on the initial tylosin concentration and the medium.

(Symposium on Bacterial Spores: Paper XIV). Spore Properties of Clostridia Occurring in Cheese

“…This effect has been observed in many species in the genus Bacillus (Gould, 1964) and Clostridium, particularly for nisin in C1. butyricum (Ramseier, 1960) and for tylosin in a putrefactive anaerobe (Poole & Malin, 1964). As soon as nisin was discovered, its use was recommended to prevent * Value to give growth from lo4 spores within 30 days; t Min concn t o inhibit growth from 10' spores within 30 days at optimum p H and temperature.…”