Some aspects of the hip arthroplasty revision. Bone defect plasty with sponge allografts

Мухаметов, У. Ф.; Mukhametov, F.F.; Suleĭmanov, I; Нагаев, Р. Я.; Нигматуллин, Р. Т.; Shangina, O. R.

doi:10.18019/1028-4427-2016-4-29-35

Cited by 2 publications

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“…В настоящее время увеличивается потребность в костнопластических материалах для оказания высокотехнологичной медицинской помощи в травматологии, ортопедии и челюстно-лицевой хирургии пациентам, нуждающимся в замещении костных дефектов и восполнении участков атрофии костной ткани разного этиопатогенеза [1,2,3,4]. Характер регенеративных процессов костной ткани при замещении костного дефекта в значительной мере определяется свойствами самого костнозамещающего материала [1,3,5].…”

Section: Introductionunclassified

In Vitro Evaluation of the Allogeneic Bone Matrix Effect on the Adipose Mesenchymal Stromal Cells Characteristics in Combined Tissue Engineering

Черданцева

Anastasieva

Алейник

et al. 2021

Traumatology and Orthopedics of Russia

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The aim of the study was to evaluate in vitro the effect of native and deproteinized compact and spongy allogenic bone matrices on the characteristics of adipose mesenchymal stromal cells (ASC) in combined tissue engineering.Material and Methods. 24 samples of native and deproteinized compact and spongy bone were examined, which were exposed to mechanical treatment, modeling, followed by sterilization of the samples by ionizing radiation and bacteriological control of sterilization. Some of the samples underwent deproteinization. The characterized cultures of human ASC were used as test cultures to assess the interaction with the bone samples. The Cytation-5 fluorescent imager and Hoechst 3334 fluorochromes (BD Pharmingen™) and calcein (Calcein AM, BD Pharmingen™) were used to characterize the degree of adhesion, migration, and viability of ASC on bone matrix samples. Matrix cytotoxicity was evaluated by MTT assay on days 1 and 7 of extraction.Results. The bone matrix samples are characterized by the absence of cytotoxicity (rank 1). ASC demonstrated good adhesion and migration on any surface of the bone matrix and preservation of cell viability during 7 days of observation. Nuclei sizes of the cells adhered to the deproteinized bone matrix of the spongy structure increased by 25–30% compared to other samples. The cells on deproteinized bone matrix had greater size (the size of the cells from nuclei 8.8 to 11.5 μm, the average size of cells nuclei from an 86.3 μm to 129,0 μm, the average perimeter of the cells nuclei from 30.7 μm to 40.7 μm) than in the native bone matrix samples.Conclusion. The results of the study of various allogeneic bone matrices demonstrate that deep purification of the bone matrix determines the absence of cytotoxicity and the most favorable conditions for the adhesion, migration, proliferation and viability of ASC. Also makes it possible to use tissue engineering based on bone matrices of different structures. Deproteinized spongy bone matrices are best suited for this purpose.

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Section: Introductionunclassified

In Vitro Evaluation of the Allogeneic Bone Matrix Effect on the Adipose Mesenchymal Stromal Cells Characteristics in Combined Tissue Engineering

Черданцева

Anastasieva

Алейник

et al. 2021

Traumatology and Orthopedics of Russia

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Vreden Russian Research Institute of Traumatology and Orthopedics

Vorobev

Божкова

Тихилов

et al. 2017

Traumatology and Orthopedics of Russia

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Currently, the use of bone allografts for reconstructive orthopedic surgery in clinical practice around the world is becoming a common procedure. Bone allografts are the first substitute material to the autologous bone and the best alternative to any artificial substituting material. The methods used for the preservation, processing and sterilization of bone are changing and evolving with time. The main goals remain the same including exclusion of infections and creation of the material with sustained properties of the normal bone.The present review reflects the essential methods for biological tissue processing, sterilization and preservation with the analysis of the key requirements for manufacturing of safe allogeneic osteoplastic materials with osteoinductive, osteoconductive and osteogenic properties.

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Some aspects of the hip arthroplasty revision. Bone defect plasty with sponge allografts

Cited by 2 publications

References 0 publications

In Vitro Evaluation of the Allogeneic Bone Matrix Effect on the Adipose Mesenchymal Stromal Cells Characteristics in Combined Tissue Engineering

In Vitro Evaluation of the Allogeneic Bone Matrix Effect on the Adipose Mesenchymal Stromal Cells Characteristics in Combined Tissue Engineering

Vreden Russian Research Institute of Traumatology and Orthopedics

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