The Laplacian matrix of a graph G is denoted by L(G) = D(G) − A(G), where D(G) = diag (d(v1), . . . , d(vn)) is a diagonal matrix and A(G) is the adjacency matrix of G. Let G1 and G2 be two graphs. A one-edge connection of two graphs G1 and G2 is a graphand v ∈ V (G2). We investigate the multiplicity of the Laplacian eigenvalue 2 of G1 ⊙uv G2, while the unicyclic graphs G1 and G2 have 2 among their Laplacian eigenvalues, by using their Laplacian characteristic polynomials. Some structural conditions ensuring the presence of the existence 2 in the G = G1 ⊙uv G2 where both G1 and G2 have 2 as Laplacian eigenvalue, have been investigated, while, here we study the existence Laplacian eigenvalue 2 in G = G1 ⊙uv G2 where at most one of G1 or G2 has 2 as Laplacian eigenvalue.