Some Characteristics of the Cold Hydrolysis Technique for Staining Plant Tissues by the Feulgen Reaction

Fox, D. P.

doi:10.1177/17.4.266

Cited by 106 publications

(39 citation statements)

References 10 publications

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“…The method of cell preparation was based on Fox (1969), with some modifications, and merely required the spreading of fish blood cells on the slides in parallel with control cells. These were fixed in Methanol-Acetic acid (3: 1), hydrolysed with 5N HCl at room temperature, stained with Schiff reagent for 1 hour, then washed in S02 water.…”

Section: Methodsmentioning

confidence: 99%

DNA Content and Chromosome Complement ofChromis Chromis(Pommacentridae, Perciformes)

1980

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Section: Methodsmentioning

confidence: 99%

DNA Content and Chromosome Complement ofChromis Chromis(Pommacentridae, Perciformes)

1980

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“…This method depends on acid hydrolysis of nuclear DNA to release aldehyde groups, which react with Schiff's reagent staining the nucleus red (Fox, 1969). Briefly, one ovary or fruit was fixed in ethanol and acetic acid solution (3: 1) for 24 h. After washing twice in water, the sample was macerated with 5 M HCl for 30 min in an ice bath followed by 60 min at 20°C.…”

Section: Cell Number and Sizementioning

confidence: 99%

Cell Wall Invertase Promotes Fruit Set under Heat Stress by Suppressing ROS-Independent Cell Death

2016

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Reduced cell wall invertase (CWIN) activity has been shown to be associated with poor seed and fruit set under abiotic stress. Here, we examined whether genetically increasing native CWIN activity would sustain fruit set under long-term moderate heat stress (LMHS), an important factor limiting crop production, by using transgenic tomato (Solanum lycopersicum) with its CWIN inhibitor gene silenced and focusing on ovaries and fruits at 2 d before and after pollination, respectively. We found that the increase of CWIN activity suppressed LMHS-induced programmed cell death in fruits. Surprisingly, measurement of the contents of H 2 O 2 and malondialdehyde and the activities of a cohort of antioxidant enzymes revealed that the CWINmediated inhibition on programmed cell death is exerted in a reactive oxygen species-independent manner. Elevation of CWIN activity sustained Suc import into fruits and increased activities of hexokinase and fructokinase in the ovaries in response to LMHS. Compared to the wild type, the CWIN-elevated transgenic plants exhibited higher transcript levels of heat shock protein genes Hsp90 and Hsp100 in ovaries and HspII17.6 in fruits under LMHS, which corresponded to a lower transcript level of a negative auxin responsive factor IAA9 but a higher expression of the auxin biosynthesis gene ToFZY6 in fruits at 2 d after pollination. Collectively, the data indicate that CWIN enhances fruit set under LMHS through suppression of programmed cell death in a reactive oxygen species-independent manner that could involve enhanced Suc import and catabolism, HSP expression, and auxin response and biosynthesis.

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“…Nuclear DNA content: for Feulgen cytophotometric estimation of 4C DNA content, ten fixed root-tips from each species were hydrolyzed in 1N HCl for 12 min at 60 o C, washed in distilled water and rinsed in SO 2 water (Fox 1969). Root tips were stained in Schiff's reagent for 2h at 14 o C and each root-tip squash was prepared in 45% lactopropionic acid.…”

Section: Methodsmentioning

confidence: 99%

Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers

Sahoo¹,

Jena²,

Mohanty³

et al. 2006

RBT

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Analysis of karyotype, nuclear DNA content and RAPD markers were performed in four species of Bruguiera (Rhizophoraceae) of Bhitarkanika mangrove forests, Orissa, India. Detailed karyotype analysis revealing 2n=34 in B. cylindrica and 2n=36 in B. gymnorrhiza was reported for the first time and 2n=34 in B. parviflora and B. sexangula was confirmed. On the basis of the common types of chromosomes present among Bruguiera, two distinct groups were found; one consists of B. cylindrica and B. parviflora and the other of B. gymnorrhiza and B. sexangula. The symmetrical karyotype with same chromosome types grouped B. cylindrica and B. parviflora together and presence of Type E chromosomes placed B. gymnorrhiza and B. sexangula in a separate group, suggesting their closer affinity in their respective group. Analysis of chromosome length, volume, INV and 4C DNA content confirmed this division. Nuclear DNA content was two-fold higher (~17.0 pg) in the second group than in the first (~8.0 pg). The amplification products generated through RAPD revealed 1-9 amplicons with size variations from 600 bp to 2 500 bp with 49.31% genetic similarity between B. gymnorrhiza and B. sexangula and 47.10% in between B. cylindrica and B. parviflora. The high copy number marker band (~ 1 100 bp) yielded in OPN-15 primer in B. parviflora the characteristic DNA marker, which was cloned and used as probes for assessment of genetic diversity, and demonstrated its close genetic affinity to B. cylindrica. B. gymnorrhiza and B. sexangula also produced similar marker bands of ~600 bp and ~2 200 bp in the same primer. All of the cytological, 4C DNA content and RAPD data confirmed the existence of two taxonomically distinct groups of Bruguiera: one consisting of B. cylindrica and B. parviflora and the other of B. gymnorrhiza and B. sexangula as placed earlier (1862) in the tribe Rhizophoreae by Bentham and Hooker, on the basis of the flowering habits of Bruguiera. Genetically, the B. sexangula and B. gymnorrhiza group was found to be very closely, rather than distantly, related to B. parviflora and B. cylindrica. Our results demonstrate that molecular markers together with cytological evidence provide an effective tool to access the existing interspecific genetic polymorphism in mangrove species, to solve the taxonomic problems and to design their conservation strategy. Rev. Biol. Trop. 55 (2): 437-448. Epub 2007 June, 29.

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Some Characteristics of the Cold Hydrolysis Technique for Staining Plant Tissues by the Feulgen Reaction

Cited by 106 publications

References 10 publications

DNA Content and Chromosome Complement ofChromis Chromis(Pommacentridae, Perciformes)

DNA Content and Chromosome Complement ofChromis Chromis(Pommacentridae, Perciformes)

Cell Wall Invertase Promotes Fruit Set under Heat Stress by Suppressing ROS-Independent Cell Death

Molecular phylogenetic relationships among four species of the mangrove tree genus Bruguiera (Rhizophoraceae), as revealed by chromosome and RAPD markers

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