Some Chemical Studies of Commercial Bacteriological Peptones

McAlpine, James G.; Brigham, George D.

doi:10.1128/jb.16.4.251-256.1928

Cited by 5 publications

(2 citation statements)

References 2 publications

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“…A scan of profiles of peptone produced by BD Biosciences (Sparks, MD, USA) and Hi Media Biosciences shows a wide choice of peptone types. The unique characteristics of each peptone product depends on the quality and source of the protein starting material, the quality and source of the enzyme, and the method of hydrolysis used to make the peptone [ 27 , 30 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, nitrate assimilation has been cited as a major factor governing the virulence of this pathogen [ 40 ]. Peptone being a major source of nitrogen supply, the availability of nitrates in the lot may be a major influencing factor [ 27 , 30 - 32 ]. It warrants further detailed investigations to understand the exact cause of such variations which is our next plan of research investigation.…”

Section: Discussionmentioning

confidence: 99%

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Significant Effects Due to Peptone in Kelman Medium on Colony Characteristics and Virulence of Ralstonia solanacearum in Tomato

Thomas¹,

Upreti²

2014

TOMICROJ

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The study was taken up to assess if the media constituents played any role in governing the variable colony characteristics or pathogenicity of the bacterial wilt pathogen, Ralstonia solanacearum cultured on the widely employed Kelman medium. The effects due to the constituents 2,3,5-triphenyl tetrazolium chloride (TTC), peptone, casein hydrolysate and glucose on colony characteristics were investigated using -80°C stored culture of strain ‘NH-Av01’ (race 1, biovar 3) isolated from tomato. Comparing the pigment inducing TTC from two brands, its source or mode of storage/incorporation did not impart any significant effects. The source of peptone, on the other hand, displayed striking effects on the extent of colony growth, fluidity and red pigmentation depending on type, brand or batch / lot of manufacture as documented with 20 different formulations. Significant differences in the pathogenicity of isolate derived from different peptone sources in seedling-challenge assay on tomato were observed. The observations on peptone effects were endorsed with four other isolates belonging to distinct geographic locations, crops (eggplant, chilli, ginger) or races (race 1 or 4). The peptone source did not influence the pathogen-responses in biovar tests but notably altered the pattern of lawn formation and inhibition zone development during antagonistic assays. Casein hydrolysate displayed some variable effects while glucose source had no effect. This study brings to light the significant modifying effects by the peptone-constituent in Kelman medium on the physiology of R. solanacearum and the virulence of isolate and the need to consider the source of media components during culture maintenance, host-pathogen interaction studies or microbe-microbe interaction investigations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Significant Effects Due to Peptone in Kelman Medium on Colony Characteristics and Virulence of Ralstonia solanacearum in Tomato

Thomas¹,

Upreti²

2014

TOMICROJ

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Human Milk Studies

Erickson

Stoner

Macy

1933

Journal of Biological Chemistry

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50. Studies in the Metabolism of the Lactic Acid Bacteria. I. Nitrogen Metabolism. J. G. Davis and A. T. R. Mattick

Davis

Mattick

1932

Journal of Dairy Research

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1. The true lactic acid bacteria cannot grow in a medium in which the sole source of nitrogen is an amino acid mixture or an ammonium salt.2. The presence of growth factors (yeast extract, marmite and peptones) does not permit the utilisation of this “simple” nitrogen.3. The streptococci grow fastest with those peptones containing the greatest amount of the higher fractions, Bactoproteose being superior to the other peptones tested; the lactobacilli, however, show a considerably greater fermentation rate with those peptones containing most amino nitrogen, Fairchild's being the best. Similarly, the streptococci thrive best upon a peptic casein digest which is about 15 days old, further digestion impoverishing the growth; the lactobacilli, however, grow better the longer the digestion. The significance of these observations is discussed from the aspects of bacterial metabolism, the sequence of flora in ripening cheese, and the preparation of casein digest media.

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Some Chemical Studies of Commercial Bacteriological Peptones

Cited by 5 publications

References 2 publications

Significant Effects Due to Peptone in Kelman Medium on Colony Characteristics and Virulence of Ralstonia solanacearum in Tomato

Significant Effects Due to Peptone in Kelman Medium on Colony Characteristics and Virulence of Ralstonia solanacearum in Tomato

Human Milk Studies

50. Studies in the Metabolism of the Lactic Acid Bacteria. I. Nitrogen Metabolism. J. G. Davis and A. T. R. Mattick

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