Some Conditions Governing Zoospore Production in Axenic Cultures of Phytophthora cinnamomi Rands

Byrt, Pauline N.; Grant, B. Rosemary

doi:10.1071/bt9790103

Cited by 75 publications

(35 citation statements)

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“…The medium for all count data of antheridial types contained 10 % cleared V8-juice, 0n01 % CaCO $ , 0n002 % β-sitosterol, 2 % Difco bacteriological agar (V8A), and was prepared as described by Byrt & Grant (1979). From the set of 72 isolates, seven which formed paragynous antheridia and two which formed only amphigynous antheridia were paired individually with the Cape Hope A1 isolate on V8A in Petri dishes and replicated four times.…”

Section: Development and Behaviour Of Paragynous Antheridiamentioning

confidence: 99%

The role of paragynous and amphigynous antheridia in sexual reproduction of Phytophthora cinnamomi

Hüberli

Tommerup

Hardy

1997

Mycological Research

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The morphology of gametangia was examined in 43 pairs of isolates (mating types A1iA2 ; 11 A1 and 24 A2 isolates ; five isozyme\electrophoretic types) of Phytophthora cinnamomi. An amphigynous antheridium always formed with each oogonium. However, in 41 of the crosses a proportion (39 had 0n2-10 % and two had 30 %) of oogonia also consistently had single or multiple paragynous antheridia. Single or multiple paragynous antheridia formed concurrently with amphigynous ones during the period of gametangial production in paired colonies. Where there were multiple paragynous antheridia associated with an oogonium, sometimes additional antheridia formed after fertilization or even after oospores were visible. Developmental studies showed that when meiosis in amphigynous and paragynous antheridia was simultaneous, fertilization tubes developed synchronously from both. However, cytological examination indicated that either a nucleus from an amphigynous or a paragynous antheridium fertilized the oosphere. Observations of paragynous and amphigynous, and amphigynous-only associations suggested that fertilization from either type of antheridium only occurred when meiosis in the oogonium was nearly synchronous with that of the antheridium. Asynchronous meiosis between oogonia and antheridia may contribute to failed fertilization and aborted oospore development. This appears to be the first description of paragynous antheridia in P. cinnamomi and the second observation of oogonia with both paragynous and amphigynous antheridia in a heterothallic Phytophthora species. Moreover, the development of both paragynous and amphigynous antheridia with an oogonium is rare in Phytophthora, as is the development of multiple antheridia. Antheridial variation is a characteristic to be taken into account in isolate identification. Nuclei from paragynous antheridia appear able to fertilize oospheres and therefore, have a role in sexual reproduction.

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Section: Development and Behaviour Of Paragynous Antheridiamentioning

confidence: 99%

The role of paragynous and amphigynous antheridia in sexual reproduction of Phytophthora cinnamomi

Hüberli

Tommerup

Hardy

1997

Mycological Research

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“…Hyphae of P. cinnamomi (DAR 522646;ATCC 200982) were cut from the growing edge of a nutrient agar culture, transferred to liquid nutrient medium (Chen and Zentmyer, 1970;Byrt and Grant, 1979), and left to grow for 2 days at 25°C. Sporangial production was initiated by washing the mycelium four times over a 4-h period with nutrient-poor mineral salts solution at 22°C .…”

Section: Culture Of Sporangiamentioning

confidence: 99%

Dynamic Rearrangement of the Filamentous Actin Network Occurs during Zoosporogenesis and Encystment in the OomycetePhytophthora cinnamomi

Jackson

Hardham

1998

Fungal Genetics and Biology

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“…For experiment 1, mycelial discs (6 mm diameter) of Phytophthora cinnamomi bearing sporangia, produced using the axenic method of Byrt & Grant (1979), were placed in 3 cm diam Petri dishes containing 5 ml sterile distilled water. The dishes were chilled for 30 min at 4 °C for synchronized zoospore release and zoospore density was determined to be 10-20 zoospores μl −1…”

Section: Inoculum and Inoculationmentioning

confidence: 99%

In planta selfing and oospore production of Phytophthora cinnamomi in the presence of Acacia pulchella

Jayasekera

McComb

Shearer

et al. 2007

Mycological Research

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This paper provides the first evidence of A2 type 1 and type 2 isolates of Phytophthora cinnamomi producing selfed oospores in planta in an Australian soil and in a potting mix. Oospores were observed in infected lupin (Lupinus angustifolius) roots incubated for 7 d either in the substrate under potted Acacia pulchella plants, or in soils collected from under and near varieties of A. pulchella in jarrah (Eucalyptus marginata) forest. The A2 type isolates varied in their ability to produce selfed oospores and none were produced by A1 isolates. The gametangial association was amphigynous and spores were predominantly spherical with diameters from 13-40 μm. The oospores were viable but dormant. Two A2 type isolates produced small numbers of selfed oospores with amphigynous antheridia axenically in Ribeiro's liquid medium within 30 d, and one A2 type 2 isolate produced oospores after mating with an A1 strain. Evidence is presented that the presence of roots of Acacia pulchella, and particularly A. pulchella var. glaberrima and var. goadbyi, enhances the production of oospores.

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Some Conditions Governing Zoospore Production in Axenic Cultures of Phytophthora cinnamomi Rands

Cited by 75 publications

References 0 publications

The role of paragynous and amphigynous antheridia in sexual reproduction of Phytophthora cinnamomi

The role of paragynous and amphigynous antheridia in sexual reproduction of Phytophthora cinnamomi

Dynamic Rearrangement of the Filamentous Actin Network Occurs during Zoosporogenesis and Encystment in the OomycetePhytophthora cinnamomi

In planta selfing and oospore production of Phytophthora cinnamomi in the presence of Acacia pulchella

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