Some considerations on quantitative methodology and detection limits in organic mass spectrometry

Abstract

Mass spectrometric sensitivity data for the compounds cholesterol, cholestane, DDT, decafluorotriphenylphosphine, morphine, LSD and methyl stearate have been developed over a period of time and under a variety of conditions. This information is presented on both a relative and an absolute basis. With the exception of morphine, relative sensitivies were reproducible to within a factor of three over a six month period. This reproducibility facilitates quantitation and a single standard can be used for calibration of organic compounds from various classes. Detection limits of 50 femtograms for LSD are realized for samples introduced via the direct inlet probe.

Analysis of thyroid hormones as their heptafluorobutyryl methyl ester derivatives by gas chromatography - mass spectrometry

“…It should be emphasized that for quantitative analysis, the calibration data of Figure 12 basis because of the possibility of variations in instrumental conditions as shown in an earlier study (21). For this purpose, multiple ion detection using an accelerating voltage alternator (AVA) (23) and simultaneous monitoring of several ions, including one or more of the isotopically labeled internal standard, is necessary.…”

Determination of mefruside and its metabolites in urine by high performance liquid chromatography

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because of the possibility of variations in instrumental conditions as shown in an earlier study (21). For this purpose, multiple ion detection using an accelerating voltage alternator (AVA) (23) and simultaneous monitoring of several ions, including one or more of the isotopically labeled internal standard, is necessary.

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