The use during recent years of immature cereal grasses such as oats, wheat, rye, and barley has received considerable attention as a protein and vitamin concentrate for both human and animal consumption, since grasses cut just previous to the jointing stage are high in protein and vitamin content. KOHLER (10) studied the effect of the stage of growth on the composition of the grasses and showed that protein, fat, chlorophyll, carotene, riboflavin, ascorbic acid, and thiamine attained a maximum concentration at, or about, the time of the jointing stage. Pantothenic acid and biotin were found to attain a maximum at a later period in the development of the plant while nicotinic acid was present in maximum concentration at an earlier stage.Considerable work has been done on the influence of nutrient deficiencies on the composition of plants. The published literature has dealt mainly with such plant constituents as proteins and carbohydrates. A great deal of work has been done on the factors concerned with vitamin C content of plants. Most of the workers agree that environmental factors, particularly light intensity, may greatly affect the ascorbic acid content. There is no general agreement, however, as to the influence of mineral nutrients on the ascorbic acid content of plants. Most of the investigations have been done oni fruits or leafy vegetables, but little study has been made of the factors influencing the ascorbic acid content of immature cereal grasses. A number of studies have been made on the effect of soil and nutrients oni the riboflavin content of cereal grain, but few investigations have been concerned with the riboflavin content of immature cereal grasses.The work described in this paper was carried out to determine the effect of single nutrient deficiencies on the ascorbic acid and riboflavin content of the immature oat plant.
Experimental proceduresCULTURAL METHODS Illinois 30-2088 oats, supplied by the Illinois Agricultural Experiment Station, were used in the experiment. The plants were grown in one-gallon 1 The expenses incurred in the present study were borne in part by a grant from the