Some factors affecting the interaction between actin in leukemic L1210 cells and DNase I

Malicka-Błaszkiewicz, Maria; Roth, Jay S.

doi:10.1016/s0006-291x(81)80173-8

Cited by 26 publications

(47 citation statements)

References 9 publications

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“…Cells were homogenized and the cytosolic fraction was prepared as described earlier by Malicka-Blaszkiewicz and Roth (1981). Cells were gently washed with PBS, scraped with a rubber policeman and suspended in freshly made monomeric actin stabilizing buffer containing 10 mM Tris-HCl, pH 7.4; 1 mM dithiothreitol; 0.1 mM ATP; 0.1 mM CaCl 2; and 0.25 M sucrose (buffer A).…”

Section: Isolation Of Cytosolic Fractionsmentioning

confidence: 99%

Gelsolin affects the migratory ability of human colon adenocarcinoma and melanoma cells

et al. 2012

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Section: Isolation Of Cytosolic Fractionsmentioning

confidence: 99%

Gelsolin affects the migratory ability of human colon adenocarcinoma and melanoma cells

et al. 2012

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“…Then the specimens were divided into two parts: one was embedded in paraffin for immunohistochemistry, and the other was subjected to homogenization according to a method reported by Malicka-Błaszkiewicz and Roth (12). Then the homogenates were centrifuged and cytosolic fraction was frozen.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Selected Enzymes with Specific Inhibitors in the Tissue Homogenates from Patients with Colorectal Cancer

Hap¹,

Kielan²,

Siewiński³

et al. 2010

Polish Journal of Surgery

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4Large intestine malignancy is the second most common malignancy and second leading cause of cancer mortality in Poland. This is related to late detection of these lesions, e.g. due to lack of effective screening tests. Lesions found by a surgeon are clinically advanced, making the treatment often ineffective and sometimes even completely impossible. Discovery of a substance that would be able to stop key processes for the development of malignancy could change such situation. Activity of certain enzymes was found to increase in malignant cells and invasion of malignancy could be triggered by inadequate amount of endogenous inhibitors of these enzymes in the surrounding healthy tissues. Inhibitors identical with that produced in human cells were found in egg whites. the aim of the study was to determine ability of cystatin isolated from egg whites to inhibit activity of cathepsin B and L. material and methods. Immunohistochemistry and histology of tissue specimen collected from malignant lesions resected from 60 patients diagnosed with large intestine adenocarcinoma, who underwent surgical treatment in 2 nd Department of General and Oncological Surgery, Medical University of Wrocław between 2007 and 2009. Results. Differences were fund between health tissues, margins and center of the malignant lesions with regard to amount and distribution of stained cathepsin B -cystatin complexes. The above mentioned inhibitors were able to inhibit 90% of primary activity of cathepsin B and L in malignant tissues. Conclusions. Cystatins obtained from egg whites could be used as substances supporting anti-cancer therapy in the future. Key words: malignancy, large intestine, cystatins, cathepsins, inhibitor therapyIncidence of large intestine malignancy is growing and it is estimated that a lifetime risk of large intestine malignancy among the worldwide population is 6%; high percentage of them will die. Cancer of the large intestine (colorectal cancer) is the second most common malignancy and second leading cause of cancer mortality in Poland, both in women and men. Its incidence in our country is increasing at a rate of 2.5% per year. Colorectal cancer is diagnosed too late in our country. Currently no screening method that would result in early detection of colorectal cancer with high sensitivity and specificity, that would be cheap and accepted by patients, is available. Studies and analyses suggest that the most effective screening programs are based on colonoscopy.

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“…Cytosol fraction was prepared as described earlier by Malicka-Błaszkiewicz and Roth [70]. The cells growing on the tissue culture dishes were gently washed with NaCl/P i buffer, then scraped with a rubber policeman and suspended in G actin stabilizing buffer (freshly prepared), containing 10 mM Tris/HCl pH 7.4, 0.25 M sucrose, 1 mM DTT, 0.1 mM ATP 0.1 mM CaCl 2 .…”

Section: Isolation Of Cytosolic Fractionsmentioning

confidence: 99%

“…The actin determination was based on its inhibitory effect on DNase I from bovine pancreas under standard assay conditions, as described by Malicka-Błaszkiewicz and Roth [70]. The concentration of monomeric actin (G) was estimated by DNase I inhibition, directly in crude cytosol sample.…”

Section: Actin Measurementsmentioning

confidence: 99%

“…The concentration of monomeric actin (G) was estimated by DNase I inhibition, directly in crude cytosol sample. Total actin (T) content was measured after dilution of samples with the G actin stabilizing buffer until maximal inhibition of DNase I was reached [70]. Filamentous actin (F) was calculated by subtracting the amount of G actin from the total actin (F = T À G).…”

Section: Actin Measurementsmentioning

confidence: 99%

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Vanadium(III) complexes with l-cysteine – stability, speciation and the effect on actin in hepatoma Morris 5123 cells

Osińska-Królicka

Podsiadły

Bukietyńska

et al. 2004

Journal of Inorganic Biochemistry

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Some factors affecting the interaction between actin in leukemic L1210 cells and DNase I

Cited by 26 publications

References 9 publications

Gelsolin affects the migratory ability of human colon adenocarcinoma and melanoma cells

Gelsolin affects the migratory ability of human colon adenocarcinoma and melanoma cells

Inhibition of Selected Enzymes with Specific Inhibitors in the Tissue Homogenates from Patients with Colorectal Cancer

Vanadium(III) complexes with l-cysteine – stability, speciation and the effect on actin in hepatoma Morris 5123 cells

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