Some features of the metastatic cancer cells in prostaglandin production.

Abstract

In order to establish metastatic lesions, 2.5>< 106 AH100B cells were injected into the left carotid artery of male Donryu rats. Each metastatic nodule in the liver or kidney, 1 mm or less in diameter, thus obtained was then injected into the peritoneal cavity in which these metastatic cells come to free. About 3 weeks later, each ascites was collected from the rats, while not bloody. Then, cancer cells obtained from each ascites were suspended in Dulbecco's PBS without Ca2+ and Mg2+ (pH 7.2) after washing. Then, 106 metastasized or control cancer cells were incubated in 0.1 ml of PBS mentioned above together with 0.1,u Ci of (1-14C)-AA at 24°C for 3 min, respectively. After the extraction procedure, AA metabolites formed were separated by means of TLC, and each TLC plate was subjected to autoradiography. In the metastasized cells, PG production ability was generally accelerated and especially in that of PGF2a as compared with that of the control. cancer metastasis ; PG production ; PGF2a ; growth factor ; promotor Metastasis to other organs is one of characteristics of cancer which makes it difficult to treat cancer patients. Therefore, it is desirable to clarify the mechanism of cancer metastasis in order to cure patients. Fidler (1973a) reported that only 1% of B16 melanoma cells injected intravenously had survived to form actual pulmonary metastasis. Fidler (1973b) succeeded also in obtaining the highly metastatic strains of B16 melanoma by means of intravenous injection and tissue culture of the metastatic lesions. Bosmann et al. (1973) recognized the biochemical features of the most highly metastatic strain (F10), that is, the change in the electrophoretic mobility, surface glycoprotein, proteases and increasing degradative enzyme as compared with those of low metastatic B16 melanoma

Some Features of Prostaglandin Synthesis of the Cancer Cells Metastasized into Liver from Gastric Cancer Lesions.

“…These results suggest that PGs produced by hepatic metastatic cancer cells might play an important role in hepatic metastatic formation. cancer metastases; liver; gastric cancerWe have been investigating features of prostaglandin (PG) synthesis of the metastatic cancer cells, using an animal model in which AH100B (a rat hepatoma cell line) cells were injected into the carotid artery of male Donryu rats (Nakazawa et al 1985(Nakazawa et al , 1989(Nakazawa et al , 1991. In this paper, we will deal with some features of PG synthesis in cancer cells metastasized into the liver from the gastric cancer lesion.…”

“…Then, each cell suspension containing 1>< 106 cancer cells was mixed with 50 ,ul of the PBS containing 0.2,uCi of [1-14C] arachidonic acid (AA) (New England Nuclear, Boston, MA, USA) and incubated at 25°C for 30 min. After the incubation, PGs formed were extracted and separated by means of thin-layer chromatography (TLC) as described previously (Nakazawa et al 1989). As authentic samples, AA (Merk, Germany), 6-ketoprostaglandin F1a (6-keto-PGF1a), prostaglandin F2a (PGF2a), prostaglandin E2 (PGE2), prostaglandin D2 (PGD2) and thromboxane B2 (TXB2) (Ono Pharmaceutical Co. Ltd., Osaka), 5,ug each per plate, were used.…”

Some Features in Prostaglandin Synthesis of the Cancer Cells Which Metastasized into Liver from Intestinal Cancer Lesions.

“…These results suggest that PGs produced by hepatic metastatic cancer cells might play an important role in cancer metastasis. cancer metastasis; prostaglandin; liver; intestinal cancer Authors (Nakazawa et al 1985(Nakazawa et al , 1989(Nakazawa et al , 1991 have reported features of PG synthesis of the metastatic cancer cells, using an animal model in which AH100B (a rat hepatoma cell line) cells were injected into the carotid artery of male Donryu rats.In this article, we will deal with some features of PGs synthesized by cancer cells which metastasized into fiver from the small intestinal cancer lesion.Materials and methods. As experimental animals, male Donryu rats (Nikon Rat Co. Ltd., Urawa) weighing 100 to 120 g were used and fed with a pellet diet, NF (Oriental Yeast Co. Ltd., Tokyo).Tumor.…”

“…These results suggest that PGs produced by hepatic metastatic cancer cells might play an important role in cancer metastasis. cancer metastasis; prostaglandin; liver; intestinal cancer Authors (Nakazawa et al 1985(Nakazawa et al , 1989(Nakazawa et al , 1991 have reported features of PG synthesis of the metastatic cancer cells, using an animal model in which AH100B (a rat hepatoma cell line) cells were injected into the carotid artery of male Donryu rats.…”

“…I. Nakazawa et al they were treated as described before (Nakazawa et al 1989), 106 cancer cells suspended in 0.05 ml of sterilized physiological saline were transplanted into muscles of the small intestine of rats, respectively. About one month later, they were sacrificed and metastatic nodules sized 1 mm or less in a diameter were collected from the liver with the intestinal tumor tissues.…”