Oxygen transfer associated with natural convection in lakes and reservoirs was examined in a series of laboratory experiments. A thin, cool surface water layer (2-3 mm in thickness) was formed by chilling the air overlying a tank of surface area 0.6 m 2 and depth 0.6 m. The surface water layer became gravitationally unstable, resulting in the formation of negatively buoyant thermal plumes, which penetrated through the total depth of the water column. The spatial distribution of oxygen concentration at the air-water interface in the tank was visualized using a fluorescence imaging technique to quantify the oxygen transfer driven by only natural convection. Pyrenebutyric acid (PBA) at a concentration of 3.0 ϫ 10 Ϫ6 mole L Ϫ1 was used as the fluorophore, and the quenching of the fluorescence by oxygen was used to produce a spatial distribution of dissolved oxygen. A light plane was generated across the tank by the refraction of a laser light beam, and two-dimensional images were continuously acquired with an intensified charge coupled-device (ICCD) camera. Analysis of these images revealed the sinking of cooled water to transport oxygen, and the experiments enabled the quantification of the oxygen transferred from the air into water at a range of heat fluxes. The results confirm that vertical penetration of cold-dense water can be a significant source of oxygen for lakes and reservoirs.''Oxygen is the most fundamental parameter of lakes, aside from water itself. . . '' (Wetzel 1975), and an improved understanding of water quality issues is dependent on understanding how dissolved oxygen (DO) enters and is distributed through these systems. Oxygen and other gases enter and leave a water body across the air-water interface. The rate at which this occurs is frequently parameterized as being a function of the wind speed across the surface and on the gas concentration in the water, relative to its saturation level (Broecker et al. 1978;O'Connor 1983). At times of high wind speed and low DO concentration, the transfer rate into the aqueous phase will be relatively high.The study of gas transfer across an air-water interface has generally been conducted with reference to the ocean (for example, Watson et al. 1991;Wanninkhof 1992). Here, sus-