Some haemato-immunological parameters in the mussel Perna perna

Barracco, Marcherita Anna; Medeiros, Igor Dias; Moreira, Fábio Mosso

doi:10.1006/fsim.1998.0196

Cited by 82 publications

(55 citation statements)

References 58 publications

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“…Although most of the haemocytes contained hydrolytic enzymes, only 6% ingested yeast in vitro. Such low activity was also reported for the mussels Perna perna (Barracco et al 1999) and Mytilus galloprovincialis (Cajaraville & Pal 1995) and might be attributable to the lack, in experimental conditions, of humoral opsonins, which β-glucuronidase 2.8 ± 1.2 3.6 ± 0.5 9.5 ± 1.8*** 5.8 ± 0.6*** Acid phosphatase 12.3 ± 1.0 25.6 ± 0.6 13.1 ± 2.1 ns 23.9 ± 3.2 ns Table 3. Chamelea gallina.…”

Section: Discussionsupporting

confidence: 71%

“…Although most of the haemocytes contained hydrolytic enzymes, only 6% ingested yeast in vitro. Such low activity was also reported for the mussels Perna perna (Barracco et al 1999) and Mytilus galloprovincialis (Cajaraville & Pal 1995) and might be attributable to the lack, in experimental conditions, of humoral opsonins, which (Cajaraville & Pal 1995. Chamelea gallina haemocytes display a good adhering ability, unlike the results reported for other venerid species (Foley & Cheng 1974.…”

Section: Discussionmentioning

confidence: 52%

“…Acid phosphatase is a typical marker for lysosomes (de Duve 1963), even though extralysosomal localisations of these enzymes have been described in several kinds of cells (Borgers & Verheyan 1985). Particularly in bivalves, a high activity of acid phosphatase was found in Ruditapes decussatus , M. galloprovincialis (Ottaviani et al 1998), Mercenaria mercenaria (Yoshino & Cheng 1976), Mya arenaria (Huffman & Tripp 1982), Crassostrea virginica (Cheng & Downs 1988), O. edulis and C. gigas (Auffret 1989), Perna perna (Barracco et al 1999), M. galloprovincialis (Bayne et al 1979, Cajaraville et al 1995, and M. edulis (Moore & Lowe 1977). Its presence was not reported in T. philippinarum (Cima et al 2002).…”

Section: Discussionmentioning

confidence: 99%

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Morphological and cytoenzymatic characterization of haemocytes of the venus clam Chamelea gallina

Pampanin

Marin²,

Ballarin³

2002

Dis. Aquat. Org.

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A morphological and enzymatic characterization of Chamelea gallina haemocytes was carried out as a prerequisite for further studies on venus clam immunobiology. Two main types of circulating haemocytes were identified (1) hyalinocytes (79.2%), agranular cells with a central nucleus surrounded by a little cytoplasm, and (2) granulocytes (16.5%), smaller granular cells with smaller nuclei. Small cells with a strongly basophilic nucleus and a thin layer of peripheral cytoplasm, probably undifferentiated blast cells (4.3%), were also observed. Both granulocytes and hyalinocytes can assume a spreading or round morphology. The enzymatic activities of haemocytes were also investigated. Some of the granulocytes and hyalinocytes were positive for hydrolytic enzymes, suggesting a role for these cells in phagocytosis; no oxidative enzymes were detected in C. gallina haemocytes. Granulocytes and hyalinocytes can easily adhere to the substratum and exhibit a low phagocytosis activity towards foreign particles (6.3%), whereas the fraction of cells containing ingested material significantly increased after pre-incubation of test particles with cell-free haemolymph, which suggests the presence of opsonin(s) in the haemolymph. KEY WORDS: Haemocytes · Chamelea gallina · Cytoenzymology · Phagocytosis Resale or republication not permitted without written consent of the publisherDis Aquat Org 49: [227][228][229][230][231][232][233][234] 2002 (Cheney 1971, Foley & Cheng 1974, Moore & Eble 1977, Huffman & Tripp 1982. Since no data are available concerning Chamelea gallina haemocytes and their role in immunity, we carried out a morpho-functional characterization of C. gallina haemocytes as a prerequisite for further studies on the immunobiology of the venus clam in order to define a series of immuno-biomarkers to be used in the evaluation of stress conditions for this species.Our results indicate the presence of 2 main haemocyte types, namely granulocytes and hyalinocytes, both exhibiting lysosomal enzymes and phagocytosis activity. MATERIALS AND METHODSClams (shell length 20 to 30 mm) were collected with a hydraulic dredge at Venice Lido, on the west coast of the Northern Adriatic Sea, in front of the Venice lagoon. They were kept in the laboratory for 2 to 3 d before experiments in 70 l tanks filled with aerated seawater that was renewed every other day (salinity 35 ± 1 ‰; temperature 17 ± 1°C) and fed with microalgae (Isochrysis galbana and Phaeodactylum tricornutum).Heamolymph collection. Haemolymph was collected from the anterior adductor muscle of clams with a syringe containing 10 mM of L-cysteine in filtered seawater (FSW), pH 7.5, to a final dilution of 1:1 haemolymph/L-cysteine. Haemolymph was also collected in the absence of L-cysteine and centrifuged at 780 × g for 10 min to pellet cells and obtain cell-free haemolymph (CFH).Haemocyte cultures. Haemolymph was centrifuged at 780 × g for 10 min and the pellet was resuspended in FSW to achieve a final concentration of 10 6 cells ml -1 . Sixty µl of haemocyte susp...

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Section: Discussionsupporting

confidence: 71%

“…Although most of the haemocytes contained hydrolytic enzymes, only 6% ingested yeast in vitro. Such low activity was also reported for the mussels Perna perna (Barracco et al 1999) and Mytilus galloprovincialis (Cajaraville & Pal 1995) and might be attributable to the lack, in experimental conditions, of humoral opsonins, which (Cajaraville & Pal 1995. Chamelea gallina haemocytes display a good adhering ability, unlike the results reported for other venerid species (Foley & Cheng 1974.…”

Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

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Morphological and cytoenzymatic characterization of haemocytes of the venus clam Chamelea gallina

Pampanin

Marin²,

Ballarin³

2002

Dis. Aquat. Org.

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“…Hemagglutination titre in serum was determined by assay according to Barracco et al [34]. Briefly, twofold serial dilutions of the serum from each sample were made with phosphate buffered saline (PBS, pH 7.2) in U-shaped bottom microtiter wells.…”

Section: Serum Immune Parametersmentioning

confidence: 99%

Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila

Chen

Liu

Jiang

et al. 2015

Fish & Shellfish Immunology

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“…The total hemocyte count (THC) shows high inter-individual variability, such as Mercenaria mercenaria (Foley and Cheng, 1975) and Sepia plana (Wootton and Pipe, 2003), while no variability in the THC is noted between the sexes of a species such as Perna perna (Barracco et al, 1999). In this study, we found that the hemocyte density in mussels gradually increased with age, from 2 years to 5 years; the average hemocyte density was 2.09 × 10 6 cells/mL, which is of the same order of magnitude as the density in Mytilus edulis, Cerastoderma edule, Ensis siliqua , Hyriopsis schlegeli (Guo et al, 2008), and Cristaria plicata (Xie et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Relationship between quality of pearl cultured in the triangle mussel Hyriopsis cumingii of different ages and its immune mechanism

Xu¹,

Li-wei²,

Xie³

et al. 2011

Aquaculture

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Some haemato-immunological parameters in the mussel Perna perna

Cited by 82 publications

References 58 publications

Morphological and cytoenzymatic characterization of haemocytes of the venus clam Chamelea gallina

Morphological and cytoenzymatic characterization of haemocytes of the venus clam Chamelea gallina

Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila

Relationship between quality of pearl cultured in the triangle mussel Hyriopsis cumingii of different ages and its immune mechanism

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