Some Insight into the Physical Basis of the Cryoprotective Action of Dimethyl Sulfoxide and Ethylene Glycol

Murthy, S. Srinivasa

doi:10.1006/cryo.1997.2064

Cited by 74 publications

(75 citation statements)

References 25 publications

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“…2 Solid curves and thin lines are VFT and Arrhenius fits, respectively. As for ethylene glycol, the calorimetric T g obtained by DSC measurements [16] is employed as T Ã g due to rapid crystallization below the melting temperature in our measurements. Solid thick line shows the behavior of f b m for glycerol estimated before [2].…”

Section: Resultsmentioning

confidence: 99%

Dielectric relaxation processes in supercooled polyhydric alcohols and their mixtures

Minoguchi

Kaneko

Sotokawa

et al. 2006

Journal of Non-Crystalline Solids

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Section: Resultsmentioning

confidence: 99%

Dielectric relaxation processes in supercooled polyhydric alcohols and their mixtures

Minoguchi

Kaneko

Sotokawa

et al. 2006

Journal of Non-Crystalline Solids

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“…45,55,[62][63][64][65][66][67][68][69] Thus, it is likely that in DMSO-water medium of moderate composition, the H-bond competition of DMSO with water and NMF favors the OD-water H-bond. As a consequence, the delocation of the water molecules H-bonded to the NMF would be lower in that case, in a good parallelism with previous results.…”

Section: Discussionmentioning

confidence: 99%

A hybrid neutron diffraction and computer simulation study on the solvation of N-methylformamide in dimethylsulfoxide

Cordeiro

Soper²

2013

The Journal of Chemical Physics

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Articles you may be interested inThe solvation of N-methylformamide (NMF) by dimethylsulfoxide (DMSO) in a 20% NMF/DMSO liquid mixture is investigated using a combination of neutron diffraction augmented with isotopic substitution and Monte Carlo simulations. The aim is to investigate the solute-solvent interactions and the structure of the solution. The results point to the formation of a hydrogen bond (H-bond) between the H bonded to the N of the amine group of NMF and the O of DMSO particularly strong when compared with other H-bonded liquids. Moreover, a second cooperative H-bond is identified with the S atom of DMSO. As a consequence of these H-bonds, molecules of NMF and DMSO are rather rigidly connected, establishing very stable dimmers in the mixture and very well organized first and second solvation shells.

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“…28 Compared to aqueous DMSO solutions, the glass transition temperature of the commonly used cryoprotective agent trehalose is comparatively higher (T g = -113.9°C). 13 Below the glass transition temperature, the mobility of molecules within the sample is reduced due to an increased viscosity of 10 13 Pa-s. Angell 29 reported that NaCl solutions cannot vitrify under ''normal'' cooling procedures.…”

Section: Physics Of Biospecimens At Low Temperaturesmentioning

confidence: 99%

Storage of Human Biospecimens: Selection of the Optimal Storage Temperature

Hubel

Spindler

Skubitz

2014

Biopreservation and Biobanking

121

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Millions of biological samples are currently kept at low tempertures in cryobanks/biorepositories for long-term storage. The quality of the biospecimen when thawed, however, is not only determined by processing of the biospecimen but the storage conditions as well. The overall objective of this article is to describe the scientific basis for selecting a storage temperature for a biospecimen based on current scientific understanding. To that end, this article reviews some physical basics of the temperature, nucleation, and ice crystal growth present in biological samples stored at low temperatures (-20°C to -196°C), and our current understanding of the role of temperature on the activity of degradative molecules present in biospecimens. The scientific literature relevant to the stability of specific biomarkers in human fluid, cell, and tissue biospecimens is also summarized for the range of temperatures between -20°C to -196°C. These studies demonstrate the importance of storage temperature on the stability of critical biomarkers for fluid, cell, and tissue biospecimens.

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Some Insight into the Physical Basis of the Cryoprotective Action of Dimethyl Sulfoxide and Ethylene Glycol

Cited by 74 publications

References 25 publications

Dielectric relaxation processes in supercooled polyhydric alcohols and their mixtures

Dielectric relaxation processes in supercooled polyhydric alcohols and their mixtures

A hybrid neutron diffraction and computer simulation study on the solvation of N-methylformamide in dimethylsulfoxide

Storage of Human Biospecimens: Selection of the Optimal Storage Temperature

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