Some like it hot, but not too hot

Greppi, Chloe; Budelli, Gonzalo; Garrity, Paul

doi:10.7554/elife.12838

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…Temperature sensation is particularly essential for small animals, such as fruit flies, whose body temperatures vary with ambient temperatures [1]. Many disease vectors, including mosquitoes, respond to the temperature of their warmblooded hosts and use it to guide their blood-feeding, through which they can transmit human diseases [2][3][4][5][6][7]. Therefore, it is crucial to understand the molecular and cellular mechanisms of temperature sensation, which may provide molecular targets to prevent host-seeking behaviors in disease vectors.…”

Section: Introductionmentioning

confidence: 99%

Ionotropic Receptor-dependent cool cells control the transition of temperature preference in Drosophila larvae

et al. 2021

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Temperature sensation guides animals to avoid temperature extremes and to seek their optimal temperatures. The larval stage of Drosophila development has a dramatic effect on temperature preference. While early-stage Drosophila larvae pursue a warm temperature, late-stage larvae seek a significantly lower temperature. Previous studies suggest that this transition depends on multiple rhodopsins at the late larval stage. Here, we show that early-stage larvae, in which dorsal organ cool cells (DOCCs) are functionally blocked, exhibit similar cool preference to that of wild type late-stage larvae. The molecular thermoreceptors in DOCCs are formed by three members of the Ionotropic Receptor (IR) family, IR21a, IR93a, and IR25a. Early-stage larvae of each Ir mutant pursue a cool temperature, similar to that of wild type late-stage larvae. At the late larval stage, DOCCs express decreased IR proteins and exhibit reduced cool responses. Importantly, late-stage larvae that overexpress IR21a, IR93a, and IR25a in DOCCs exhibit similar warm preference to that of wild type early-stage larvae. These data suggest that IR21a, IR93a, and IR25a in DOCCs navigate early-stage larvae to avoid cool temperatures and the reduction of these IR proteins in DOCCs results in animals remaining in cool regions during the late larval stage. Together with previous studies, we conclude that multiple temperature-sensing systems are regulated for the transition of temperature preference in fruit fly larvae.

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Section: Introductionmentioning

confidence: 99%

Ionotropic Receptor-dependent cool cells control the transition of temperature preference in Drosophila larvae

et al. 2021

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Responses to Temperatures of DifferentDrosophilaSpecies

Huda

Vaden

Omelchenko

et al. 2021

Preprint

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Temperature is a critical environmental variable that affects the distribution, survival, and reproduction of most animals. Although temperature receptors have been identified in different animals, how these receptors respond to temperatures is largely unknown. Here we use modified single-fly thermotactic assays to analyze movements and temperature preferences of nine Drosophila species. The ability/inclination to move varies among these species and at different temperatures. Importantly, different species prefer various ranges of temperatures. While wild-type D. melanogaster flies avoid the warm temperature in the warm avoidance assay and the cool temperature in the cool avoidance assay, D. bipectinata and D. yakuba avoid neither warm nor cool temperatures and D. biarmipes and D. mojavensis do not avoid the warm temperature in the warm avoidance assay. These results demonstrate that Drosophila species have different mobilities and temperature preferences, thereby benefiting the research on molecular mechanisms of temperature responsiveness.

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Responses of differentDrosophilaspecies to temperature changes

Huda

Omelchenko

Vaden

et al. 2022

Journal of Experimental Biology

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Temperature is a critical environmental variable that affects the distribution, survival and reproduction of most animals. Although temperature receptors have been identified in many animals, how these receptors respond to temperature is still unclear. Here, we describe an automated tracking method for studying the thermotactic behaviors of Drosophila larvae and adults. We built optimal experimental setups to capture behavioral recordings and analyzed them using free software, Fiji and TrackMate, which do not require programming knowledge. Then, we applied the adult thermotactic two-choice assay to examine the movement and temperature preferences of nine Drosophila species. The ability or inclination to move varied among these species and at different temperatures. Distinct species preferred various ranges of temperatures. Wild-type D. melanogaster flies avoided the warmer temperature in the warm avoidance assay and the cooler temperature in the cool avoidance assay. Conversely, D. bipectinata and D. yakuba did not avoid warm or cool temperatures in the respective assays, and D. biarmipes and D. mojavensis did not avoid the warm temperature in the warm avoidance assay. These results demonstrate that Drosophila species have different mobilities and temperature preferences, which will benefit further research in exploring molecular mechanisms of temperature responsiveness.

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Some like it hot, but not too hot

Abstract: A temperature-sensitive receptor prevents mosquitoes from being attracted to targets that are hotter than a potential host.

Cited by 3 publications

References 10 publications

Ionotropic Receptor-dependent cool cells control the transition of temperature preference in Drosophila larvae

Ionotropic Receptor-dependent cool cells control the transition of temperature preference in Drosophila larvae

Responses to Temperatures of DifferentDrosophilaSpecies

Responses of differentDrosophilaspecies to temperature changes

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