Some New Methods for Affixing Sections to Glass Slides. Ii. Organic-Solvent Based Adhesives

1987

Stain Technology

Self Cite

A variety of polymeric formulations have been evaluated for use as pressure-sensitive adhesives in affixing sections of fresh or frozen tissues for histochemistry and other purposes. The best adhesive with respect to water resistance, refractive index, and low temperature tack was polyisobutylene. In mixtures of different molecular weights this adhesive is specially suited for room temperature or cryostat use. Slides coated with this adhesive should only be used with staining or incubation solutions which have been filtered and degassed. Mounting cannot be performed with xylene-containing media, UV-curable monomeric media being favored. For anhydrous processing or nonpermanent preparations, polyethyleneimines, which are soluble in water but insoluble in xylene, may be used. Mixtures of polydimethylsiloxanes can be applied for subsequent temporary mounting with water or aqueous media.

“…1, 3, 4, 5). Furthermore, even the more waterresistant types tended to separate from plain glass slides during exposure to water, so that only primed slides (Fink 1987b) were used.…”

Section: Results and Discusionmentioning

confidence: 99%

Section: Results and Discusionmentioning

confidence: 99%

Some New Methods for Affixing Sections to Glass Slides. III. Pressure-Sensitive Adhesives

1987

Stain Technology

Self Cite

A solvent-free coating-procedure for the improved preparation of cryostat sections in light microscope histochemistry

1992

Histochemistry

A new technique is presented for the external stabilization of cryostat sections by spraying the specimen surfaces with an aqueous solution of poly(vinyl alcohol) before each sectioning stroke. The spray freezes upon the surface and forms a tough coating which facilitates subsequent sectioning and handling especially of difficult material. The sections are affixed upon cold glass slides covered with an improved formulation of pressure-sensitive adhesive. During further processing of the affixed sections, the PVA-coating and any surrounding supporting medium dissolve without traces in the first aqueous incubation or staining solution.

A new integrated concept for the improved preparation of sections of fresh or frozen tissue for light microscope histochemistry

1986

Histochemistry

In order to obtain thin sections of plant tissues which combined good morphological preservation and the preservation of the substances and enzyme activities in the tissues, a concept of section preparation by external stabilization was developed. The main components are as follows: appropriate supporting medium; surface coating before each sectioning process, the coating being either non-permanent, permanent, or semi-permanent; suitable techniques for affixing the coated sections to the slides using either pressure-sensitive adhesive or solvent-based adhesive; and mounting media with defined refractive indices (preferably UV-curable, water-soluble monomers). By this approach, sections exhibiting excellent morphological and physiological preservation were obtained using either a cryostat at -30 degrees C or a rotary microtome at room temperature.