Some observations on the adsorption of tetracyclines to glass and plastic labware

Ciarlone, Alfred E.; Fry, Bill W.; Ziemer, Donna

doi:10.1016/0026-265x(90)90050-f

Cited by 19 publications

(6 citation statements)

References 5 publications

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“…Furthermore, TCs may be lost during sample preparation and may degrade storage; hence, it is important to analyze the samples for TCs within 48 h of extraction (Tso et al, 2011). Where possible, polypropylene or polyethylene were substituted for glass because of a significantly lower adsorption in these materials relative to glass (Ciarlone et al, 1990). When the use of glass was unavoidable, glassware was washed in 10% nitric acid to strip away any residual organic material or free metals that may reduce recovery or promote adsorption.…”

Section: Resultsmentioning

confidence: 99%

“…Three methods were examined for liquid manure. Volumes of 5-mL manure samples were added to 15-mL polypropylene centrifuge tubes for all methods to minimize sorption of TCs (Ciarlone et al, 1990). Exploratory procedures were adapted from previously reported methods (Hamscher et al, 2002;Ho et al, 2012) with minor modifications (see Supplemental Material S1, S2).…”

Section: Liquid Manurementioning

confidence: 99%

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Enhancing Extraction and Detection of Veterinary Antibiotics in Solid and Liquid Fractions of Manure

Wallace

Aga

2016

J. Environ. Qual.

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Analysis of veterinary antibiotics in separated liquid and solid fractions of animal manures is vital because of wide variations in the composition of agriculturally applied manure. Differentiation of antibiotic concentrations is important between liquid and solid manures, as their sorption onto the solid fraction depends on physicochemical properties of each antibiotic and manure composition (e.g., organic content, pH) and because each fraction may be treated and reused differently. Here, an efficient and sensitive method for the analysis of 22 veterinary antibiotics in the liquid and solid fractions of manure is reported. Tetracycline (TC), macrolide, and sulfonamide antibiotics were extracted from liquid manure by liquid-liquid extraction (LLE) with methanol following acidification with acetic acid. Extraction from solids was performed by sonication with acetonitrile, methanol, and 0.1 M EDTA-McIlvaine buffer. Cleanup of extracts was achieved by solid-phase extraction with hydrophilic-lipophilic balance (HLB) cartridges or tandem amino (NH2) and HLB cartridges. Quantification of antibiotics was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) under wrong-way-round (WWR) ionization for sulfonamides and TCs and right-way-round ionization for macrolides. Recoveries of 58 to 94.7% and 62 to 94.3% were obtained in liquid and solid manure, respectively. Method detection limits range from 1.2 to 12 ng L and 0.5 to 7.9 μg kg dry wt. in liquids and solids, respectively. This method allows for extraction and analysis of both mobile antibiotics in liquid phase and hydrophobic antibiotics adsorbed on the solids. Without separate analysis, antibiotic concentrations may be improperly estimated by analyzing whole manure, as reported in many studies to date.

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Section: Resultsmentioning

confidence: 99%

Section: Liquid Manurementioning

confidence: 99%

Enhancing Extraction and Detection of Veterinary Antibiotics in Solid and Liquid Fractions of Manure

Wallace

Aga

2016

J. Environ. Qual.

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“…A minority of farmers reported using spectinomycine (aminoglycosides, 5.9%), sulfadimethoxine (sulfonamides, 5.9%), cephalexin monohydrate (penicillins, 5.9%), kanamycin sulfate (aminoglycosides, 5.9%), and chloramphenicol (phenicol, 5.9%). Even though tetracyclines were used frequently, their sampling and analysis requires avoiding glassware and using plastic apparatus due to their strong binding to borosilicate glassware [ 24 ], which prevented the monitoring and simultaneous determination of other antibiotics. Therefore, after consideration of usage frequency, physico-chemical properties (water solubility, octanol-water partition coefficient, soil organic carbon-water partition coefficient, acid dissociation constant, Table 1 ), and access to analytical equipment, sulfamethoxazole (SMX), sulfadiazine (SDZ), trimethoprim (TRIM) and enrofloxacine (ENRO) were selected for monitoring in water.…”

Section: Methodsmentioning

confidence: 99%

Occurrence and Dissipation of the Antibiotics Sulfamethoxazole, Sulfadiazine, Trimethoprim, and Enrofloxacin in the Mekong Delta, Vietnam

et al. 2015

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The Mekong Delta in Vietnam has seen a rapid development and intensification of aquaculture in the last decades, with a corresponding widespread use of antibiotics. This study provides information on current antibiotic use in freshwater aquaculture, as well as on resulting antibiotic concentrations in the aquatic environment of the Mekong Delta. Two major production steps, fish hatcheries and mature fish cultivation, were surveyed (50 fish farm interviews) for antibiotic use. Different water sources, including surface water, groundwater and piped water (164 water samples) were systematically screened for antibiotic residues. To better understand antibiotic fate under tropical conditions, the dissipation behavior of selected antibiotics in the aquatic environment was investigated for the first time in mesocosm experiments. None of the investigated antibiotics were detected in groundwater and piped water samples. Surface water, which is still often used for drinking and domestic purposes by local populations, contained median concentrations of 21 ng L-1 sulfamethoxazole (SMX), 4 ng L-1 sulfadiazine (SDZ), 17 ng L-1 trimethoprim (TRIM), and 12 ng L-1 enrofloxacin (ENRO). These concentrations were lower than the predicted no effect concentrations (PNECs) and minimum inhibitory concentrations (MICs), suggesting limited antibiotic-related risk to aquatic ecosystems in the monitored systems. The dissipation half-lives of the studied antibiotics ranged from <1 to 44 days, depending on the availability of sunlight and sediment. Among the studied antibiotics TRIM was the most persistent in water systems. TRIM was not susceptible to photodegradation, while the dissipation of ENRO and SDZ was influenced by photolysis. The recorded dissipation models gave good predictions of the occurrence and concentrations of TRIM, ENRO and SDZ in surface water. In summary, the currently measured concentrations of the investigated antibiotics are unlikely to cause immediate risks to the aquatic environment, yet the persistence of these antibiotics is of concern and might lead to chronic exposure of aquatic organisms as well as humans.

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“…We hypothesized that marinopyrrole A in solution was partially binding to plastic, a phenomenon described for other antibiotics, including doxycycline and minocycline (4). This event, called sorption, can occur when a drug is initially adsorbed and subsequently absorbed by its plastic container (4,15), which may be ascribed to the very hydrophobic and slightly acidic nature of marinopyrrole A. To examine this possibility, the loss of marinopyrrole A to the walls of polystyrene versus glass tubes was measured by methanol extraction of adsorbed drug and UV spectrometric quantification at A 324 .…”

mentioning

confidence: 99%

Pharmacological Properties of the Marine Natural Product Marinopyrrole A against Methicillin-Resistant Staphylococcus aureus

Haste

Hughes

Tran

et al. 2011

Antimicrob Agents Chemother

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The ongoing spread of methicillin-resistant Staphylococcus aureus (MRSA) strains in hospital and community settings presents a great challenge to public health and illustrates the urgency of discovering new antibiotics. Marinopyrrole A is a member of a structurally novel class of compounds identified from a species of marine-derived streptomycetes with evidence of antistaphylococcal activity. We show that marinopyrrole A has potent concentration-dependent bactericidal activity against clinically relevant hospital-and communityacquired MRSA strains, a prolonged postantibiotic effect superior to that of the current first-line agents vancomycin and linezolid, and a favorable resistance profile. Marinopyrrole A showed limited toxicity to mammalian cell lines (at >20؋ MIC). However, its antibiotic activity against MRSA was effectively neutralized by 20% human serum. A variety of marinopyrrole analogs were isolated from culture or synthetically produced to try to overcome the inhibitory effect of serum. While many of these compounds retained potent bactericidal effect against MRSA, their activities were also inhibited by serum. Marinopyrrole A has significant affinity for plastic and may therefore have potential as a potent anti-MRSA agent in cutaneous, intracatheter, or antibiotic-lock applications.

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Some observations on the adsorption of tetracyclines to glass and plastic labware

Cited by 19 publications

References 5 publications

Enhancing Extraction and Detection of Veterinary Antibiotics in Solid and Liquid Fractions of Manure

Enhancing Extraction and Detection of Veterinary Antibiotics in Solid and Liquid Fractions of Manure

Occurrence and Dissipation of the Antibiotics Sulfamethoxazole, Sulfadiazine, Trimethoprim, and Enrofloxacin in the Mekong Delta, Vietnam

Pharmacological Properties of the Marine Natural Product Marinopyrrole A against Methicillin-Resistant Staphylococcus aureus

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