Some observations on the metabolism of bacteria oxidizing molecular hydrogen

Kluyver, A. J.; Manten, A.A.

doi:10.1007/bf02272768

Cited by 49 publications

(18 citation statements)

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“…In cells grown heterotrophically, these effects were observed as decreased levels of the two vital autotrophic functions, the hydrogen-oxidizing and carbon dioxide-fixing systems. Among the members of the genus Hydrogenomonas, H. flava (9), H. facilis (13,20,23), H. ruhlandii (16), and Hydrogenomonas H 1 (2) possess inducible or repressible hydrogenases.…”

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Autotrophic and Heterotrophic Metabolism of Hydrogenomonas: Regulation of Autotrophic Growth by Organic Substrates

Stukus

DeCicco

1970

J Bacteriol

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The effects of a number of organic substrates on the autotrophic metabolism of Hydrogenomonas eutropha were examined. Dual substrate (mixotrophic) cultivation in the presence of hydrogen plus either fructose or alanine allowed autotrophic growth to begin immediately after the exhaustion of the organic substrate. On the other hand, the presence of acetate, pyruvate, or glutamate caused a lengthy lag to occur before autotrophic growth commenced. With acetate or pyruvate this lag (plateau) in the dicyclic growth curve was due to the repression of ribulose diphosphate carboxylase (RDPC) synthesis during mixotrophic growth. During heterotrophic growth with glutamate, RDPC was partially repressed; however, during mixotrophic growth, RDPC activity was high. Thus the delay of autotrophic growth was not due to a repression of RDPC by glutamate. The data suggest that glutamate interferes with autotrophic metabolism by repressing the incorporation of inorganic nitrogen. The repression of these vital autotrophic functions by acetate, pyruvate, and glutamate occurred both in the presence and absence of hydrogen, i.e., during both heterotrophic and mixotrophic cultivation. The derepression of the affected systems during the plateau phase of the dicycic growth curves was demonstrated. Carbon dioxide assimilation by whole cells agreed well with the RDPC activity of extracts from cells grown under similar conditions. 339 on July 16, 2020 by guest

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confidence: 99%

Autotrophic and Heterotrophic Metabolism of Hydrogenomonas: Regulation of Autotrophic Growth by Organic Substrates

Stukus

DeCicco

1970

J Bacteriol

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“…Among the hydrogen autotrophs other than mycobacteria, some possess inducible (1,4,12,17,22) and some constitutive hydrogen-oxidizing systems (7,20). The RuDP carboxylase systems in the facultative autotrophs all seem to be inducible or repressible and appear to be a major mechanism regulating autotrophic metabolism.…”

Section: Discussionmentioning

confidence: 99%

Hydrogenase and ribulose diphosphate carboxylase during autotrophic, heterotrophic, and mixotrophic growth of scotochromogenic mycobacteria

Park¹,

DeCicco²

1976

J Bacteriol

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Two key autotrophic enzyme systems, hydrogenase and ribulose diphosphate carboxylase, were examined in Mycobacterium gordonae and two other chemolithotrophic, scotochromogenic mycobacteria under different cultural conditions. In all three organisms both enzymes were inducible and were produced in significant levels only in the presence of the specific substrate, hydrogen or carbon dioxide. M. gordonae exhibited increased growth rates and yields, indicating mixotrophic growth, in the presence of a number of single organic substrates, including acetate, pyruvate, glucose, fructose, and glycerol. In contrast to other aerobic hydrogen autotrophs, the presence of either acetate or pyruvate did not repress ribulose diphosphate carboxylase, and mixotrophic growth was rapid with these substrates. In the absence of carbon dioxide, growth in glycerol medium under an atmosphere of hydrogen and oxygen was severely inhibited, even with cells preadapted to heterotrophic growth on glycerol. Cyclic adenosine monophosphate was not effective in inducing hydrogenase or carboxylase in heterotrophic, mixotrophic, or hydrogen-inhibited cultures.

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“…9. Rods 1.5 /Lm in length; motile by 1 polar flagellum (rarely 2); flagellar fine structure of type I (Aragno et aI., 1977 Kluyver and Manten, 1942).…”

Section: Chapter 70mentioning

confidence: 99%