Some physical and biological properties of 4-thiouridine- and dihydrouridine-deficient tRNA from chloramphenicol-treated Escherichia coli

Waters, Larry C.; Shugart, Lee R.; Yang, Wen; Best, Audrey N.

doi:10.1016/0003-9861(73)90332-9

Cited by 36 publications

(22 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of two additional, clearly defined species of tRNAPhe from BD-cellulose chromatography, one appears to be deficient in the 2-methylthio modification; the other species appears to be deficient in that modification, in the isopentenyl modification, and in at least one other modification. Studies of tRNAPhe during recovery of E. coli from treatment with CP indicate a precursor relationship of the various peaks, in agreement with results recently reported by Waters et al (17), and a possibly obligatory sequence of modification forming the fully modified ms2i"A.…”

supporting

confidence: 90%

“…Further support for that possibility is based on the accumulation of species IV when cells are starved for cysteine or methionine. Also, it was reported that CP tRNA (E. coli B) has less than the normal amount of ms2i "A which apparently is replaced by i6A [N6-(A2_ igopentenyl)adenosine] (17). tRNA from methionine-starved E. coli (rel-) has appreciable quantities of i6A and possibly s2i6A (2-thio-N6-(Ml-isopentenyl)adenosine) but only a small amount of the fully modified nucleoside ( P. F. Agris, D. J. Armstrong, K. P. Schiifer, and D. Sill, manuscript in preparation).…”

Section: Discussionmentioning

confidence: 99%

“…While methylthio and isopentenyl modifications appear to influence strongly the chromatographic behavior of tRNAPhe and thus are modifications more easily studied indirectly, other modifications could also be missing in tRNAPhe from metabolically stressed cells. Results from this laboratory (8) and Waters et al (17) indicate that E. coli, in which RNA synthesis proceeds while protein synthesis is inhibited either by chloramphenicol or by star-vation or a relstrain for a required amino acid, produces tRNA deficient in dihydrouridine and 4-thiouridine. It should be noted that the degree of undermodification of tRNA from inhibited cells differs with different strains.…”

Section: Discussionmentioning

confidence: 99%

“…Waters (16) suggested that the isoaccepting species of tRNAPhe appearing in response to CP are intermediates in the maturation of tRNA. The inhibition of protein synthesis in E. coli by CP or by amino acid starvation of a rel-strain leads to a decrease in the amount of dihydrouridine and 4-thiouridine in bulk tRNA synthesized under those conditions (8,17). Additional species of tRNAPhe also are observed in bacteria grown with limited aeration (19) or at low iron concentrations (13,20).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Precursor relationship of phenylalanine transfer ribonucleic acid from Escherichia coli treated with chloramphenicol or starved for iron, methionine, or cysteine

Juárez¹,

Skjold²,

Hedgcoth³

1975

J Bacteriol

View full text Add to dashboard Cite

When treated with chloramphenicol, Escherichia coli 15T-produces two new species (IV and V) of transfer ribonucleic acid specific for phenylalanine in addition to the major normal species (II) and two minor normal species (I and III), which are seen as distinct components upon fractionation by chromatography on columns of benzoylated diethylaminoethyl-cellulose. Species IV is produced when cells are grown in iron-deficient medium and is, therefore, probably deficient in the 2-methylthio modification of N6-(A2-isopentenyl)adenosine. A new minor species (Va) also appears under those conditions. All of the new components elute earlier than the major normal species. Addition of chloramphenicol to iron-deficient cells leads to the production of species V, and that production is blocked by rifampin, as is the production of species IV. Thus, species IV and V appear to be transcriptional products. Although E. coli 15Tappears to be rel+, starvation for methionine or cysteine leads to the accumulation of species IV (without addition of chloramphenicol); rifampin blocks the accumulation. Species V is still produced on addition of chloramphenicol to starved cultures. Starvation for arginine or tryptophan does not alter the chromatographic profile from the normal case. Treatment with permanganate indicates that species II and IV contain isopentenyladenosine but that species V does not. Species V appears to be deficient in both isopentenyl and methylthio modifications of adenosine and perhaps at least one other modification, because removing the isopentenyl moiety from adenosine does not convert species IV into species V, but converts it into species Va. A precursor relationship among species V, IV, and II is suggested by following the chromatographic profile of phenylalanine transfer ribonucleic acid during recovery of E. coli from treatment with chloramphenicol; the various species increase and decrease in a sequential manner.

show abstract

supporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Precursor relationship of phenylalanine transfer ribonucleic acid from Escherichia coli treated with chloramphenicol or starved for iron, methionine, or cysteine

Juárez¹,

Skjold²,

Hedgcoth³

1975

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…In this last regard changes in the isoacceptor patterns have been observed for a number of Escherichia coli tRNA's derived from cells cultured under conditions leading to unbalanced growth. These conditions include: amino acid starvation (2,4,13,25), treatment with chloramphenicol (16,24) or amino acid analogues (2), iron deficiency (27), limited aeration (27), low levels of phosphate (17), and deviation from standard culturing temperatures (2). It is not known if the intracellular condition responsible for the changes observed is common to all of the conditions cited, neither is the biochemical basis of these and other changes understood except in three special cases involving amino acid starvation.…”

mentioning

confidence: 99%

Unbalanced growth and the production of unique transfer ribonucleic acids in relaxed-control Escherichia coli

Kitchingman¹,

Fournier²

1975

J Bacteriol

View full text Add to dashboard Cite

The unique leucine-, arginine-, valine-, and phenylalanine-specific transfer ribonucleic acids (tRNA's) produced in relaxed-control (rel-) Escherichia coli during leucine or arginine starvation are chromatographically similar to those produced by chloramphenicol treatment. The major unique relleucine-specific and phenylalanine-specific tRNA's are heterogeneous, accumulate with time of starvation, and can account for up to 70% of the respective amino acid acceptor activities. The changes which occur in the isoacceptor profiles for tRNAIAU and tRNAPhe as a function of starvation time suggest that the unique species are undermodified precursors to the major isoacceptor species observed in nonstarved cells. Analyses of the isoacceptor patterns of tRNA from cells recovering from starvation suggest that the unique species of tRNAIeU and tRNAPhe may not be normally occurring precursors. When leucine-starved cells were incubated in fresh, fully supplemented medium, the major unique tRNALeU and tRNAPhe appeared to be converted to normal species only slowly or not at all. The results are consistent with the view that some of the events in the post-transcriptional modification of tRNA may occur in an ordered sequence. An examination of the subcellular distribution of the unique leucine and phenylalanine tRNA's revealed that these species occur on the ribosome at about the same frequency as the major, normally occurring isoacceptor species. This result provides additional evidence of a precursor-product relationship for the unique and normal tRNA's and further indicates that there is no discrimination against the unique species by the ribosome.

show abstract

Chemical Nature, Properties, Location, and Physiological and Pathological Variations of Modified Nucleosides in tRNAs

Dirheimer

1983

Modified Nucleosides and Cancer

View full text Add to dashboard Cite

Some physical and biological properties of 4-thiouridine- and dihydrouridine-deficient tRNA from chloramphenicol-treated Escherichia coli

Cited by 36 publications

References 32 publications

Precursor relationship of phenylalanine transfer ribonucleic acid from Escherichia coli treated with chloramphenicol or starved for iron, methionine, or cysteine

Precursor relationship of phenylalanine transfer ribonucleic acid from Escherichia coli treated with chloramphenicol or starved for iron, methionine, or cysteine

Unbalanced growth and the production of unique transfer ribonucleic acids in relaxed-control Escherichia coli

Chemical Nature, Properties, Location, and Physiological and Pathological Variations of Modified Nucleosides in tRNAs

Contact Info

Product

Resources

About