Some physical-chemical and biological properties of the rod-shaped coliphage M13

Filamentous bacterial viruses

“…For several different types of duplex DNA, Kleinschmidt spreading on water gives 0. 35 (40,126,127,143,162). The buoyant density of Ff viral DNA is 1.722 g/cm3 in CsCl, relative to 1.710 g/cm3 for Escherichia coli DNA, and 1.447 g/cm3 in Cs2SO4, relative to 1.426 g/cm3 for E. coli DNA (180).…”

“…This effect may result from the low level of suppression by UAA suppressors (70), or it may result from a virus-induced alteration in the mechanism of translation (58) (19,87,127,135,162). DNA can also be isolated by alkali treatment followed by banding in a CsCl density gradient (53,157 (127) and was dialyzed at a concentration of about 300 jxg/ml against 0.2 M NaCI, 0.02 M tris (hydroxymethyl)aminomethane (Tris), 0.002 M ethylenediaminetetraacetic acid (EDTA) (pH 7.5) and then against 0.2 M NaCI, 0.02 M Tris (pH 7.5; dialysis tubing was presoaked in EDTA).…”

“…Another source of this hyperchromicity could be n-,w* transitions in the DNA (53 (87,190,205). Plaque-forming ability is resistant to deoxyribonuclease and ribonuclease and is moderately resistant to Pronase and trypsin; however, it is sensitive to the proteolytic enzymes Nagarse, ficin, subtilisin, and papain (132,135,158,162,168,191).…”

“…2). There is no release of f3-galactosidase Ba teria from infected cells (85,140,162). Thus, an early > definition (54) Cell number (turbidity or colony formers) continues to increase in infected cultures, but the b07 growth rate is lower than in uninfected cultures.…”

“…Thus, an early > definition (54) Cell number (turbidity or colony formers) continues to increase in infected cultures, but the b07 growth rate is lower than in uninfected cultures. In general, turbid-plaque strains inhibit bacterial growth least in liquid culture, and clear-plaque strainsinhibitmost (35,85,95,129,132,135,162 Garen) was grown at 37 C in tryptone medium (127) cells give smaller colonies than non-infected cells. with aeration.…”