Some probiotic properties of yeast isolates from infant faeces and Feta cheese

Psomas, Evdoxios; Andrighetto, Christian; Litopoulou-Tzanetaki, E.; Lombardi, Angiolella; Tzanetakis, N.

doi:10.1016/s0168-1605(01)00580-3

Cited by 95 publications

(96 citation statements)

References 22 publications

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“…In this work, all the assayed strains were able not only to resist gastrointestinal passage but also to grow under these conditions. Other authors have reported the same results with S. cerevisiae strains isolated from infant faeces and feta cheese (Psomas et al, 2001) and with S. cerevisiae var boulardii strain isolated from food (van der Aa Kuhle et al, 2005). However, the use of bacteria has demonstrated a very low recovery after being subjected to these gastrointestinal in vitro conditions (Gusils et al, 2002).…”

Section: S Cerevisiae Strainssupporting

confidence: 61%

Binding of Aflatoxin B1 to Lactic Acid Bacteria and Saccharomyces cerevisiae in vitro: A Useful Model to Determine the Most Efficient Microorganism

Pizzolitto¹,

Bueno²,

Armando³

et al. 2011

Aflatoxins - Biochemistry and Molecular Biology

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Section: S Cerevisiae Strainssupporting

confidence: 61%

Binding of Aflatoxin B1 to Lactic Acid Bacteria and Saccharomyces cerevisiae in vitro: A Useful Model to Determine the Most Efficient Microorganism

Pizzolitto¹,

Bueno²,

Armando³

et al. 2011

Aflatoxins - Biochemistry and Molecular Biology

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“…The bile tolerance of strains was evaluated based on the decimal increase of yeasts by estimating the number of viable cells after 0 and 72 h of incubation on YPD agar plates [20]. The bile tolerance was expressed as a percentage (%) = (increment of cells in broth with bile/increment of cells in broth without bile) × 100.…”

Section: Bile Tolerancementioning

confidence: 99%

“…To determine the transit tolerance of yeast strains through simulated gastric juice, the method of Psomas was used with slight modifications [20]. Thirty milliliters of the selected yeast strains were centrifuged at 3000× g (10 min, 4°C).…”

Section: In Vitro Survival In Gastric Juicementioning

confidence: 99%

“…Several published studies have indicated that some yeast strains isolated from dairy products or human feces have been considered as potential probiotics, as they can survive in the low pH and bile environment of the gastrointestinal tract [11,14,20,21]. It has been reported that Saccharomyces boulardii was effective as a biotherapeutic agent in the prevention or treatment of antibioticassociated diarrhea and human colitis, especially for the treatment of acute diarrhea in children [25].…”

Section: Introductionmentioning

confidence: 99%

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Screening for the potential probiotic yeast strains from raw milk to assimilate cholesterol

Chen

Maubois

et al. 2010

Dairy Sci. Technol.

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-Consumption of dairy products containing probiotics with cholesterol-lowering activity has been proposed as a means to lower serum cholesterol. In the present work, 19 yeast strains, isolated from raw milk, were tested to obtain potential probiotic yeasts for assimilating cholesterol. During in vitro tests, 17 yeast strains were capable of growth in bile salt solutions, and most of the yeast strains tolerated low pH, surviving in gastric juice. Among the 19 strains assessed, Geotrichum sp. BY2 and Pichia kudriavzevii BY10 showed highest adhesive ability to HT-29 cells. All yeast strains were able to assimilate cholesterol in the range of 3.6-44.4% over a 72 h incubation, and seven of the yeast strains were significantly higher at assimilating cholesterol (P < 0.05). According to these results, the yeast strains P. fermentans BY5, P. kudriavzevii BY10, P. kudriavzevii BY15 and Yarrowia lipolytica HY4 may serve as potential probiotics to assimilate cholesterol in the human intestine.Résumé -Cribable du potentiel d'assimilation du cholestérol de souches probiotiques de levure isolées à partir de lait cru. La consommation de produits laitiers contenant des probiotiques ayant une activité hypo-cholestérolémiante a été proposée comme moyen d'abaisser le cholestérol sanguin. Dans la présente étude, 19 souches de levure isolées à partir de lait cru ont été testées comme probiotiques potentiels pour assimiler le cholestérol. Au cours des tests in vitro, Article published by EDP Sciences 17 souches étaient capables de croissance dans des solutions de sels biliaires, et la plupart des souches tolérait bien les faibles pH, en survivant dans le jus gastrique. Parmi les 19 souches testées, Geotrichum sp. BY2 et Pichia kudriavzenii BY10 montraient la plus grande capacité d'adhésion aux cellules HT-29. Toutes les souches de levure étaient capables d'assimiler le cholestérol à hauteur de 3,6 à 44,4 % après une incubation de 72 h et 7 souches avaient des taux d'assimilation significativement plus élevés (P < 0,05). D'après ces résultats, les souches de levure Pichia fermentans BY5, P. kudriavzenii BY10, P. kudriavzenii BY15 et Yarrowia lipolytica HY4 pourraient être utilisées comme probiotiques potentiels pour assimiler le cholestérol au niveau de l'intestin chez l'Homme.levure / probiotique / lait cru / cholestérol

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“…The RAPD, already successfully used to discriminate a specific yeast strain from other natural strains present in the environment (De Clercq et al, 2003;Psomas et al, 2001), failed to identify a specific DNA fragment for strain O. A cluster analysis based on RAPD results showed a close relatedness between Means and standard deviation (S.D.)…”

Section: Discussionmentioning

confidence: 99%

Development of real-time PCR using Minor Groove Binding probe to monitor the biological control agent Candida oleophila (strain O)

Massart

Clercq

Salmon

et al. 2005

Journal of Microbiological Methods

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A real-time PCR assay using a 3V -Minor Groove Binding (MGB) probe was developed for specific detection and monitoring of Candida oleophila (strain O), a biocontrol agent against Botrytis cinerea and Penicillium expansum, on harvested apples. The application of the RAPD technique on C. oleophila strains followed by reproducible sequence characterized amplified region (SCAR) amplifications allowed the identification of a semi-specific fragment of 244 bp, observed in the profiles of strain O and three other C. oleophila strains. After sequencing, polymorphisms (3%) were observed between the strain O sequence and the three other sequences. A 3V -Minor Groove Binding probe was designed to specifically match a region of the strain O sequence and was able to discriminate a single base mutation or a two-base difference in the corresponding sequences of the non-target strains. This specific detection method was applied to monitor strain O population, recovered by a washing buffer, from harvested apples. Population densities were calculated using an external standard curve consisting in a serial dilution of strain O cells in the washing buffer from untreated apples. Linearity in the standard curve was kept between 1.64Â10 2 and 1.64Â10 5 cfu cm À2 of apple surface. During a first practical experiment, the calculated population densities were similar to those obtained by plating on semi-selective media. This new real-time PCR method is a promising tool to monitor quickly and specifically strain O population on apple surface in middle-or large-scale experiments. D

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Some probiotic properties of yeast isolates from infant faeces and Feta cheese

Cited by 95 publications

References 22 publications

Binding of Aflatoxin B1 to Lactic Acid Bacteria and Saccharomyces cerevisiae in vitro: A Useful Model to Determine the Most Efficient Microorganism

Binding of Aflatoxin B1 to Lactic Acid Bacteria and Saccharomyces cerevisiae in vitro: A Useful Model to Determine the Most Efficient Microorganism

Screening for the potential probiotic yeast strains from raw milk to assimilate cholesterol

Development of real-time PCR using Minor Groove Binding probe to monitor the biological control agent Candida oleophila (strain O)

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