Some properties of Na+K+ ATPase in the follicle cells of Rhodnius prolixus

Ilenchuk, T. Toney; Davey, K. G.

doi:10.1016/0020-1790(82)90056-7

Cited by 46 publications

(21 citation statements)

References 12 publications

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“…In the previtellogenic follicle, Scatchard analysis demonstrates that JH decreased the Kd for ouabain binding, but did not alter the B,,, value, indicating that no large scale unmasking of latent binding sites occurred. Such an observation is consistent with an earlier observation of a modest increase in ATPase activity in preparations from previtellogenic follicles (8).…”

Section: Discussionsupporting

confidence: 89%

“…This action of JH is blocked by ouabain, a specific inhibitor of Na+,~+-ATl?ase (7). The homone brings about a threefold increase in activity of Na+,K+-ATPase when added to microsomal preparations of follicle cells from vitellogenic follicles, but fails to affect rnicrosomes prepared from brain (7,8). Curiously, although JH does not affect patency in previtellogenic follicles, it nevertheless brings about some increase in activity of Na+, Kf -ATPase (8), and isolated cells from previtellogenic follicles decrease in volume in the presence of JW (9).…”

mentioning

confidence: 95%

“…For personal use only. described in detail elsewhere (8). In brief, vitellogenic (400-1500 pm in length) and previtellogenic (less than 400 pm in length) follicles were removed from mated female 8. prolixus on the 4th day after feeding, and the follicular epithelium was removed from the oocytes and ground in a glass homogenizer in Rhodnius saline (1 1 ) .…”

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confidence: 99%

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Juvenile hormone increases ouabain-binding capacity of microsomal preparations from vitellogenic follicle cells

Ilenchuk¹,

Davey²

1983

Can. J. Biochem. Cell Biol.

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A comparison has been made of the effects of juvenile hormone (JH) on the binding characteristics for ouabain of microsomes prepared from brain and from cells of the follicular epithelium surrounding previtellogenic or vitellogenic oocytes in Rhodnius. JH has no effect on the binding of ouabain to brain microsomes and decreases the Kd, but does not alter the Bmax for previtellogenic follicle cells. For vitellogenic follicle cells. Scatchard analysis reveals a curvilinear relationship, which is interpreted as indicating that a new population of JH-sensitive ouabain-binding sites develops as the follicle cell enters vitellogenesis. These results are related to earlier data obtained on the effect of JH on ATPase activity, volume changes in isolated follicle cells, and the development of spaces between the cells of the follicular epithelium.

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Section: Discussionsupporting

confidence: 89%

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confidence: 95%

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Juvenile hormone increases ouabain-binding capacity of microsomal preparations from vitellogenic follicle cells

Ilenchuk¹,

Davey²

1983

Can. J. Biochem. Cell Biol.

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“…After centrifugation, the supernatant was used for determination of trypsin [47], chymotrypsin [47], lipase [48], amylase [49], alkaline phosphatase (AKP) [50] and Na + , K + -ATPase activities [51]. Gamma-glutamyl transpeptidase (γ-GT) activities in the intestine were determined by using a γ-GT Assay Kit (Sigma, St Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

Soybean β-Conglycinin Induces Inflammation and Oxidation and Causes Dysfunction of Intestinal Digestion and Absorption in Fish

et al. 2013

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β-conglycinin has been identified as one of the major feed allergens. However, studies of β-conglycinin on fish are scarce. This study investigated the effects of β-conglycinin on the growth, digestive and absorptive ability, inflammatory response, oxidative status and gene expression of juvenile Jian carp (Cyprinus carpio var. Jian) in vivo and their enterocytes in vitro. The results indicated that the specific growth rate (SGR), feed intake, and feed efficiency were reduced by β-conglycinin. In addition, activities of trypsin, chymotrypsin, lipase, creatine kinase, Na+,K+-ATPase and alkaline phosphatase in the intestine showed similar tendencies. The protein content of the hepatopancreas and intestines, and the weight and length of the intestines were all reduced by β-conglycinin. β-conglycinin increased lipid and protein oxidation in the detected tissues and cells. However, β-conglycinin decreased superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx) and glutathione reductase (GR) activities and glutathione (GSH) content in the intestine and enterocytes. Similar antioxidant activity in the hepatopancreas was observed, except for GST. The expression of target of rapamycin (TOR) gene was reduced by β-conglycinin. Furthermore, mRNA levels of interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and transforming growth factor-β (TGF-β) genes were increased by β-conglycinin. However, β-conglycinin increased CuZnSOD, MnSOD, CAT, and GPx1b gene expression. In conclusion, this study indicates that β-conglycinin induces inflammation and oxidation, and causes dysfunction of intestinal digestion and absorption in fish, and finally reduces fish growth. The results of this study provide some information to the mechanism of β-conglycinin-induced negative effects.

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“…The activities of trypsin and lipase were measured according to the methods of Wang et al (2014) and Bier (1955). The Na + -K + ATPase activity was tested by the method of Ilenchuk and Davey (1982). And the alkaline phosphatase (AKP) activity was assayed using the procedure of Krogdahl et al (2003).…”

Section: Digestive Enzymes Activities Analysismentioning

confidence: 99%

Effects of dietary vitamin A on growth, hematology, digestion and lipometabolism of on-growing gibel carp (Carassius auratus gibelio var. CAS III)

et al. 2016

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Some properties of Na+K+ ATPase in the follicle cells of Rhodnius prolixus

Cited by 46 publications

References 12 publications

Juvenile hormone increases ouabain-binding capacity of microsomal preparations from vitellogenic follicle cells

Juvenile hormone increases ouabain-binding capacity of microsomal preparations from vitellogenic follicle cells

Soybean β-Conglycinin Induces Inflammation and Oxidation and Causes Dysfunction of Intestinal Digestion and Absorption in Fish

Effects of dietary vitamin A on growth, hematology, digestion and lipometabolism of on-growing gibel carp (Carassius auratus gibelio var. CAS III)

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