Some remarks on the interpretation of the spectral properties of prodan

Balter, Aleksander; Nowak, Wiesław; Pawełkiewicz, W.; Kowalczyk, Andrzej

doi:10.1016/0009-2614(88)87067-2

Cited by 124 publications

(121 citation statements)

References 22 publications

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“…The probe emits an intense, single, broad fluorescence band, strongly red-shifted upon increasing the polarity-polarizability (p*) and the hydrogen-donor ability (a) of the media. [32,[37][38][39] We have demonstrated by using absorption and emission spectroscopies (stationary, time-resolved emission spectroscopy (TRES) and time-resolved area-normalized emission spectroscopy (TRANES)) that PRODAN in AOT reverse micellar media undergoes a partition process between the external nonpolar solvent and the RM interface. The molecular probe located in the nonpolar organic solvent always emits from a locally excited (LE) state whereas PRODAN located at the RM interface can emit from LE or ICT or both states (dual fluorescence) at room temperature depending on the AOT RM interface properties (see Scheme 2).…”

Section: Introductionmentioning

confidence: 99%

Comparison between Two Anionic Reverse Micelle Interfaces: The Role of Water–Surfactant Interactions in Interfacial Properties

Quintana¹,

Falcone²,

Silber³

et al. 2011

ChemPhysChem

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The water/sodium bis(2-ethylhexyl) phosphate (NaDEHP) reverse micelle (RM) system is revisited by using, for the first time, molecular probes to investigate interface properties. The solvatochromic behavior of 1-methyl-8-oxyquinolinium betaine (QB) and 6-propionyl-2-(N,N-dimethyl)aminonaphthalene (PRODAN) in the water/NaDEHP/toluene system is studied, and the results are compared with those obtained in water/sodium 1,4-bis(2-ethylhexyl) sulfosuccinate (AOT)/toluene RM media. The results demonstrate that the micropolarity, microviscosity, interfacial water structure, molecular probe partition, and intramolecular electron-transfer processes are dramatically altered for NaDEHP RM interfaces in comparison to the AOT systems. Because of organic nonpolar solvent penetration into the interface, NaDEHP RM media offer an interface with lower micropolarity and microviscosity than AOT media. Also, the interfacial water in the NaDEHP system shows enhanced water-water hydrogen-bond interaction in comparison with bulk water. The AOT RM interface represents a unique environment for PRODAN to undergo dual emission.

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Section: Introductionmentioning

confidence: 99%

Comparison between Two Anionic Reverse Micelle Interfaces: The Role of Water–Surfactant Interactions in Interfacial Properties

Quintana¹,

Falcone²,

Silber³

et al. 2011

ChemPhysChem

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“…The reported lifetime components in methanol are ∼2.0 ns (75%) and 3.4 ns (25%). 22 The fluorescence behavior is more complex in water than in other solvents: Balter et al 22 observed an additional, weak emission band around 430 nm. They also reported a biexponential fluorescence lifetime (τ 1 ∼0.5 ns and τ 2 ∼1.9 ns) with an additional small contribution from a long-lived component of ∼13 ns at the blue edge of the emission spectrum, exciting at 337 nm.…”

Section: Introductionmentioning

confidence: 99%

Solvation Dynamics of the Fluorescent Probe PRODAN in Heterogeneous Environments: Contributions from the Locally Excited and Charge-Transferred States

2009

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The coexistence of different excited states with different properties of the same chromophores could have significant consequences for the accurate characterization of solvation dynamics in a heterogeneous environment, such as a protein. The purpose of this work is to study the contributions of the locally excited (LE) and charge-transferred (CT) states of the fluorescent probe molecule 6-propionyl-2-(N,Ndimethylamino)naphthalene (PRODAN) to its solvation dynamics in the heterogeneous environment provided by reverse micelles formed by sodium 1,4-bis-(2-ethylhexyl) sulfosuccinate (AOT)/n-heptane/ water. We have found that the LE and CT states of PRODAN solvate on different time scales in reverse micelles (2 and ∼0.4 ns, respectively), consistent with results suggested in the literature, and have concluded that PRODAN's use as a probe of heterogeneous environments must be used with caution and that, more importantly, the same caution must be exercised with any chromophore capable of emitting from different excited states. ReceiVed: June 1, 2009; ReVised Manuscript ReceiVed: July 20, 2009 The coexistence of different excited states with different properties of the same chromophores could have significant consequences for the accurate characterization of solvation dynamics in a heterogeneous environment, such as a protein. The purpose of this work is to study the contributions of the locally excited (LE) and charge-transferred (CT) states of the fluorescent probe molecule 6-propionyl-2-(N,N-dimethylamino)naphthalene (PRODAN) to its solvation dynamics in the heterogeneous environment provided by reverse micelles formed by sodium 1,4-bis-(2-ethylhexyl) sulfosuccinate (AOT)/n-heptane/water. We have found that the LE and CT states of PRODAN solvate on different time scales in reverse micelles (2 and ∼0.4 ns, respectively), consistent with results suggested in the literature, and have concluded that PRODAN's use as a probe of heterogeneous environments must be used with caution and that, more importantly, the same caution must be exercised with any chromophore capable of emitting from different excited states.

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“…179 The dipole moment of PRODAN changes by ~5-8 D upon excitation. 180,181 A change in dipole moment of this magnitude, along with its hydrogen bonding capability, 181 makes PRODAN a suitable probe for monitoring REES effects to characterize hydrophobic binding sites in proteins. For example, PRODAN binds erythroid spectrin with a high affinity.…”

Section: Extrinsic Fluorophoresmentioning

confidence: 99%

Novel Insights Into Protein Structure and Dynamics Utilizing the Red Edge Excitation Shift Approach

Raghuraman

Kelkar

Chattopadhyay

Reviews in Fluorescence 2005

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A shift in the wavelength of maximum fluorescence emission toward higher wavelengths, caused by a corresponding shift in the excitation wavelength toward the red edge of the absorption band, is termed the red edge excitation shift (REES). This effect is mostly observed with polar fluorophores in motionally restricted media such as viscous solutions or condensed phases where the dipolar relaxation time for the solvent shell around a fluorophore is comparable to or longer than its fluorescence lifetime. REES arises from slow rates of solvent relaxation (reorientation) around an excited state fluorophore which depends on the motional restriction imposed on the solvent molecules in the immediate vicinity of the fluorophore. Utilizing this approach, it becomes possible to probe the mobility parameters of the environment itself (which is represented by the relaxing solvent molecules) using the fluorophore merely as a reporter group. Further, since the ubiquitous solvent for biological systems is water, the information obtained in such cases will come from the otherwise 'optically silent' water molecules. This makes REES extremely useful since hydration plays a crucial modulatory role in the formation and maintenance of organized molecular assemblies such as folded proteins in aqueous solutions and biological membranes. The application of REES as a powerful tool to monitor the organization and dynamics of a variety of soluble, cytoskeletal, and membrane-bound proteins is discussed.

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Some remarks on the interpretation of the spectral properties of prodan

Cited by 124 publications

References 22 publications

Comparison between Two Anionic Reverse Micelle Interfaces: The Role of Water–Surfactant Interactions in Interfacial Properties

Comparison between Two Anionic Reverse Micelle Interfaces: The Role of Water–Surfactant Interactions in Interfacial Properties

Solvation Dynamics of the Fluorescent Probe PRODAN in Heterogeneous Environments: Contributions from the Locally Excited and Charge-Transferred States

Novel Insights Into Protein Structure and Dynamics Utilizing the Red Edge Excitation Shift Approach

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