Some Results of Study Concerning in Vitro Techniques of Breeding and Improvement of Prunus Domestica L.

Barbulescu, A.; Neamtu, M.; Ilie, A.; Petcu, A.; Ion, L.; Popa, Gabriela

doi:10.17660/actahortic.2009.814.31

Cited by 2 publications

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“…Higher efficiency of rhizogenesis of plum varieties on a nutrient artificial medium -MS -Murashige and Skoog (1962), as indicated by the authors working in this direction, there is a need to adjust it taking into account varietal characteristics [5,[7][8][9]. For example, a two-fold diluted MS nutrient medium is proposed [12,13]. The composition of the nutrient medium for reproduction and rhizogenesis can include substances of auxin nature with different concentrations [7,14,15].…”

Section: Introductionmentioning

confidence: 99%

“…The composition of the nutrient medium for reproduction and rhizogenesis can include substances of auxin nature with different concentrations [7,14,15]. Most of the work on the cultivation of domestic plums was carried out on two to five varieties, while using a small number of modifications of nutrient media, but emphasizing the need for individual selection of its components [3,4,7,[11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Adjustment of culture media to optimize in vitro rhizogenesis of domestic plums of various origins

Kovalenko¹,

Поливара

2021

BIO Web Conf.

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This paper presents the results of the adjustment of 18 modifications of culture medium based on MS medium composition – Murashige and Skoog (1962) for in vitro rhizogenesis of 10 varieties of domestic plum. There were used 4 nutrient media with a full amount of macro- and microelements – MS1 with a different combination of phytohormones, 1 medium without hormones – MSc, 13 media – MS2 with half the amount of macronutrients, differing in hormonal composition. It was found that the maximum number of rooted microshoots from 21.2 to 52.2 % was on MS2–8 medium, from 11.2 to 43.1 % on MS2–5. The analysis showed that into the medium increases the percentage of microplants by 12-17 % comparing with the medium MS2–5 and MS2–7 without FA. The distinctiveness of nutrient media by the type of auxin (IAA, NAA, IBA) made it possible to clarify that IBA is the most optimal of the auxins, and the concentration of 1.7 mg/l is borderline for the growth reactions of plum varieties. It was revealed that in vitro root formation depends not only on the compositions of growth substances in the nutrient medium, but also on the genotype of the variety.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adjustment of culture media to optimize in vitro rhizogenesis of domestic plums of various origins

Kovalenko¹,

Поливара

2021

BIO Web Conf.

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Acclimatization of Plantlets fromIn vitroto the Ambient Environment

Preece

2010

Encyclopedia of Industrial Biotechnology

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Acclimatizing, hardening‐off, or conditioning plantlets from the in vitro to the ambient environment can be a challenge that may result in death or damage to a large percentage of micropropagated plants. When grown in the high humidity, low light environment often encountered in vitro, leaves have altered anatomy; poor cuticle development, with low amounts of epicuticular waxes; and the stomatal apparatus functions poorly with stomata remaining open, even in darkness or in stressful conditions. Gradually acclimatizing the plantlets to the ex vitro environment using mist, fog, or high humidity combined with shading will reduce losses. Light levels can be gradually raised and relative humidity gradually lowered as the plantlets grow new leaves and their shoots elongate. Eventually, plants will thrive in ambient conditions. Soil additives, such as mycorrhizal fungi combined careful attention to sanitation will also aid survival.

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Some Results of Study Concerning in Vitro Techniques of Breeding and Improvement of Prunus Domestica L.

Cited by 2 publications

References 0 publications

Adjustment of culture media to optimize in vitro rhizogenesis of domestic plums of various origins

Adjustment of culture media to optimize in vitro rhizogenesis of domestic plums of various origins

Acclimatization of Plantlets fromIn vitroto the Ambient Environment

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