Some rumen bacteria degrading fructan

Ziołecki, A.; Guczynska, W.; Wojciechowicz, Maria

doi:10.1111/j.1472-765x.1992.tb00774.x

Cited by 30 publications

(42 citation statements)

References 9 publications

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“…All chemicals were of analytical grade. Timothy grass fructan was isolated and purifi ed according to Ziołecki et al (1992).…”

Section: Chemicalsmentioning

confidence: 99%

“…However, neither fructanolytic species of rumen microbiota nor their enzymes involved in degradation of fructose polymers in this highly specifi c ecosystem have been well recognized to date. The previous investigation carried out at our laboratory showed that some strains of rumen treponemes were presumably able to synthesize β-fructofuranosidase (Ziołecki et al, 1992;Guczyńska, 1999). Further studies revealed, however, that strain S of these microorganisms synthesized a specifi c endolevanse and unspecifi c β-fructofuranosidase (Kasperowicz and Michałowski, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Digestion and utilization of fructose polymers by the rumen bacterium Treponema sp. strain T

Kasperowicz¹,

Michałowski²

2007

J. Anim. Feed Sci.

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In this experiment, strain T of ruminal treponemes was studied regarding the ability to utilize β-2,6-and β-2,1-fructose polymers as well as sucrose and fructose. Bacteria were able to grow on laboratory-prepared timothy grass fructan and commercial inulin, but not on sucrose or fructose. The optical density of cultures growing on inulin and timothy grass fructan was 0.5 and 1.1 absorbance units, respectively. Bacteria utilized up to 80% of the timothy grass fructan and only 30% of inulin during a 24 h incubation period. It was found that bacteria utilized only the β-2,1-inulooligosaccharides present in the commercial inulin while the long-chain polysaccharides remained undigested. Enzymatic studies showed that the bacterial cell-free extract degraded the timothy grass fructan, inulin and sucrose, releasing 13.4-19.1, 11.1-14.9 and 42.4-52.9 μM fructose/mg protein/h, respectively. It is suggested that enzymes involved in the degradation of fructose polymers could form a complex of which the enzyme specifi cally digesting the timothy grass fructan is associated with the outer membrane and acts on cell surfaces, while unspecifi c β-fructofuranosidase occupies an intracellular space and digests only the carbohydrates inside the bacterial cells. It is also suggested that such a localization of enzymes could be responsible for the inability of bacteria to utilize long-chain inulin. It is supposed that the examined bacteria are lacking in mechanism(s) transporting sucrose and fructose from the growth medium to the intracellular space of these microorganisms.

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“…All chemicals were of analytical grade. Timothy grass fructan was isolated and purifi ed according to Ziołecki et al (1992).…”

Section: Chemicalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Digestion and utilization of fructose polymers by the rumen bacterium Treponema sp. strain T

Kasperowicz¹,

Michałowski²

2007

J. Anim. Feed Sci.

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“…To be useful as a food source, any fructan must be metabolized at rates commensurate with the rate of passage through the rumen and must be linked to the utilization of ammonia from protein metabolism within the rumen. Although the degradability of some plant fructans by isolated rumen bacteria and protozoa has previously been reported (Anand, 1972; Paster and CanaleParola, 1985;Thomas, 1960;Ziolecki et al, 1992), there is no information on either the overall rate of inulin digestion or the degradability of the large bacterial levans within the rumen. Nor has the effect of levan or inulin on NH 3 metabolism in the rumen been reported.…”

mentioning

confidence: 99%

In vitro digestion of bacterial and plant fructans and effects on ammonia accumulation in cow and sheep rumen fluids.

Biggs

Hancock

1998

J. Gen. Appl. Microbiol.

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One of the problems encountered with the pasture plants perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.) as a food source for ruminants is their relatively low levels of non-structural carbohydrates (NSC) as compared to protein. As a consequence, a significant proportion of dietary protein is metabolized to meet energy requirements and large amounts of ammonia are produced, which need to be excreted (Moller et al., 1993). This represents a loss of N and a wastage of energy. Increasing the level of dietary carbohydrate is one of the means currently being explored to correct this protein/carbohydrate imbalance in pasture plants (Beever and Thorp, 1997).We are investigating the potential of fructans to provide an alternate supply of NSC by genetic manipulation of pasture plants, as genes for levan biosynthesis are now available (van der Meer et al., 1994). Fructans are a group of fructose polymers, containing either β(2-1) or β(2-6) fructosyl-fructose linkages, synthesized by some plants and certain bacteria. In general, plant fructans (inulins and phleins) are relatively small with an average degree of polymerization (DP) of around 40 (Pontis and del Campillo, 1985). In contrast, those from bacteria (levans) are very large (DPϾ100,000; Keith et al., 1991). To be useful as a food source, any fructan must be metabolized at rates commensurate with the rate of passage through the rumen and must be linked to the utilization of ammonia from protein metabolism within the rumen. Although the degradability of some plant fructans by isolated rumen bacteria and protozoa has previously been reported (Anand, 1972; Paster and CanaleParola, 1985;Thomas, 1960;Ziolecki et al., 1992), there is no information on either the overall rate of inulin digestion or the degradability of the large bacterial levans within the rumen. Nor has the effect of levan or inulin on NH 3 metabolism in the rumen been reported.This study was undertaken to investigate the metabolism of fructans in rumen fluids from both cow and sheep. The results obtained indicate that inulin and novel bacterial levan have the potential for enhancing the food quality of forage plants for ruminants.Chicory root inulin a β(2-1) plant fructan, and levan, a β(2-6) bacterial fructan from Erwinia herbicola, were obtained from Sigma Chemicals, St. Louis, MO, USA. AnalAR grade fructose and sucrose were from BDH Limited (UK). Rumen fluid was collected from randomly selected four-year-old Romney sheep and from a 16-year-old Friesian cow grazed on identical white clover and ryegrass swards. Rumen samples were strained into an airtight container, sealed to exclude oxygen and placed into an anaerobic cabinet at 39°C in a 95% CO 2 : H 2 atmosphere. Fructan and other carbohydrates were dissolved in artificial saliva (McDougall, 1948) and aliquots of rumen fluid added to give final carbohydrate concentrations of 2 mg/ml, and a rumen fluid dilution of 1 in 5. The final concentration of sucrose was 4 mg/ml (fructose equivalent 2 mg/ml). Control samples containe...

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“…Butyrivibrio fibrisolvens strain 3 bacteria isolated from ovine rumen (Ziołecki et al, 1992) were cultured in an anaerobic medium (Hungate, 1969) supplemented with 0.5% (w/v) of timothy grass fructan, inulin, inulo-oligosaccharides, sucrose, or fructose as the sole source of energy. The bacteria were grown for 24 h. At the end of the incubation period, the optical density of the cultures was determined by measuring absorbance at 660 nm and the carbohydrate concentration in the medium was determined by the anthrone method.…”

Section: Methodsmentioning

confidence: 99%

“…In contrast, very little is known about the ability of these bacteria to utilize fructans (Ziołecki et al, 1992), which are important storage carbohydrates in grasses and Compositae and consist of fructofuranosyl residues linked by either β-2,6 (levans) or β-2,1 (inulins) glycosidic bonds. The objective of this study was, therefore, to examine the ability of B. fibrisolvens to utilize various fructose polymers.…”

Section: Introductionmentioning

confidence: 99%

Preliminary assessment of the capability of the rumen bacterium, Butyrivibrio fibrisolvens, to utilize fructose polymers for growth

Cendrowska¹,

Kasperowicz²,

Michałowski³

2006

J. Anim. Feed Sci.

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The rumen bacterium Butyrivibrio fibrisolvens was able to grow in vitro when timothy grass fructan, inulin, inulooligosaccharides, sucrose, or fructose was the sole source of carbon in the culture medium, and utilized 89.9, 47.7, 80.9, 87.9 and 94.5% of the carbohydrate, respectively. The distinctly lower utilization of inulin than other carbohydrates was accompanied by a considerably lower bacteria count in the cultures. Examination of an extract from bacterial cells showed that its fructanolytic activity was closely related to the carbon source in the growth medium. The number of fructanolytic enzymes synthesized by the bacteria depended on the carbon source in the growth medium.

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Some rumen bacteria degrading fructan

Cited by 30 publications

References 9 publications

Digestion and utilization of fructose polymers by the rumen bacterium <i>Treponema</i> sp. strain T

Digestion and utilization of fructose polymers by the rumen bacterium <i>Treponema</i> sp. strain T

In vitro digestion of bacterial and plant fructans and effects on ammonia accumulation in cow and sheep rumen fluids.

Preliminary assessment of the capability of the rumen bacterium, <i>Butyrivibrio fibrisolvens</i>, to utilize fructose polymers for growth

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