1963
DOI: 10.1021/bi00905a017
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Some Stereospecificity Studies with Tritiated Pyridine Nucleotides*

Abstract: The stereospecificity of hydrogen transfer for the pyridine nucleotide has been ascertained for a number of hitherto uninvestigated dehydrogenase reactions. These determinations have been made with the help of appropriate reference enzymes, and of DPN and TPN labeled with tritium at the 4 position of the nicotinamide ring. Evidence is presented that the following enzymes use the same position (i.e., A or a) as yeast alcohol dehydrogenase: DPN-linked formic dehydrogenase from pea seeds, DPN-linked glyoxylic red… Show more

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Cited by 70 publications
(13 citation statements)
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“…NAD+-43H (spec. activity 0.7 mc/mmole) was prepared by the method of Krakow et al (38). 1 The detrltiation of NADH was followed by measuring the amount of tritium in water isolated from the reaction mixture as described by Lee et al (37).…”
Section: Stereospedfieity Of Nadh Oxidation In Mltochondriamentioning
confidence: 99%
“…NAD+-43H (spec. activity 0.7 mc/mmole) was prepared by the method of Krakow et al (38). 1 The detrltiation of NADH was followed by measuring the amount of tritium in water isolated from the reaction mixture as described by Lee et al (37).…”
Section: Stereospedfieity Of Nadh Oxidation In Mltochondriamentioning
confidence: 99%
“…The eluate was filtered and evaporated under reduced pressure at 50-60 "C. The residue was dissolved in 10 ml of water and the pyridine nucleotide was precipitated by addition of 100 ml of a mixture of ethanol and acetone (1 : 1, v/v) containing 0.05 ml concentrated HC1. The precipitate was collected by centrifugation, washed with acetone and then ether, and dried in vucuo at room temperature [12]. Recovery of the tritiated pyridine nucleotide was 120 pmol, which represents 30% yield.…”
Section: Preparation Of [4-pr0-r-~ Hinadph and [4-pros3 H I N A Dphmentioning
confidence: 99%
“…The isotope effect was further shown by mixing tritium-labeled 5~-[24-2H,]cholestane-3a,7a,12a-triol with tritiumlabeled 5j3-[24-1H2]cholestane-3a,7a,12a-triol. 24Hydroxylation of this mixture yielded 5jhholestane-3a,7a,12a,24-tetrol with a ratio of unlabeled to labeled molecules which was about 4 times higher than the ratio in the 5j3-cholestane-3a,7a,l2a-triol incubated ( Table 2). The isotope effect was essentially unchanged when the hydroxylation was inhibited by about 40°/, in an atmosphere of 40' 1, carbon monoxide.…”
Section: 0)mentioning
confidence: 98%