Some toxicological aspects op methamidophos exposure in mice<sup>1</sup>

Zayed, S.H.A.D.; Fakhr, I. M. I.; EL‐Magraby, Somaia

doi:10.1080/03601238409372444

Cited by 5 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Total residues in fruits and leaves determined at intervals following application revealed the greater to rats is 900 mg.kg" 1 ), especially fish (Szeto et al, 1982 is 13 mg.kg" 1 ) (Ware, 1989) is several times more toxic than acephate (Spencer, 1982;Maroni et al, 1990), and was reported as a potent inhibitor of plasma and RBCcholinesterases (Zayed et al, 1984). Methamidophos, besides acute intoxication, has caused delayed neuropathy in man through the mechanism of "neuropathy target esterase" (NTE) inhibition (Senanayake and Johnson, 1982).…”

mentioning

confidence: 98%

Analysis and fate of acephate and its metabolite, methamidophos, in pepper and cucumber

Antonįous

1995

J. of Env. Sc. & Hlth., Part B

View full text Add to dashboard Cite

Levels of acephate (Orthene R ) and its principle metabolite, methamidophos, in/on greenhouse-grown pepper and cucumber fruits and leaves in relation to the applied methamidophos were monitored.Dislodgeable and total residues of acephate and methamidophos were determined by gas-liquid chromatography equipped with a flame ionization detector (GC-FID) and were confirmed by nitrogen phosphorus detector (GC-NPD). The dissipation curves of the residues followed first-order kinetics (R 2 > 0.96). Initial residues of acephate on fruits varied between pepper (15.12 ppm) and cucumber (2.16 ppm) .Total residues in fruits and leaves determined at intervals following application revealed the greater * 378 ANTONIOUS persistence of acephate on pepper fruits (half-life [t 1/2 ] of 6 d) than on cucumber fruits (t 1/2 was 3.7 d) . T 1/2 values for the applied methamidophos were 4.7 and 5.3 d on pepper and cucumber fruits, respectively. Deacetylation of acephate (formation of its metabolite) was detectable 1 d following acephate treatment and reached a maximum of 2.05% of initial acephate residues 3 d after application on pepper fruits. On cucumber fruits, acephate metabolite reached a maximum of 2.12% one wk following application. No acephate residues were detected above the limit of detection of 0.001 ppm in pepper fruits 50 d following acephate application while its metabolite was detectable at that time (detectability limit was 0.0001 ppm).

show abstract

mentioning

confidence: 98%

Analysis and fate of acephate and its metabolite, methamidophos, in pepper and cucumber

Antonįous

1995

J. of Env. Sc. & Hlth., Part B

View full text Add to dashboard Cite

show abstract

“…Methamidophos (MAP) is an OP and a potent AChE inhibitor used to control insects that plague a variety of crops such as brassica, cotton, tobacco, sugar beet, lettuce, potatoes, and tree fruits (WHO, 1993). MAP is highly toxic to aquatic organisms (Tomlin, 1994) and mice (Zayed et al, 1984). It also has anticholinesterase activity in humans (Worek et al, 2007(Worek et al, , 2004.…”

Section: Introductionmentioning

confidence: 99%

Isatin-3-N4-benzilthiosemicarbazone, a non-toxic thiosemicarbazone derivative, protects and reactivates rat and human cholinesterases inhibited by methamidophos in vitro and in silico

Barcelos

Portella

Lugokenski

et al. 2012

Toxicology in Vitro

View full text Add to dashboard Cite

Organophosphates (OPs), which are widely used as pesticides, are acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitors. The inactivation of AChE results in the accumulation of acetylcholine at cholinergic receptor sites, causing a cholinergic crisis that can lead to death. The classical treatment for OP poisoning is administration of oximes, but these compounds are ineffective in some cases. Here we determined whether the new compound isatin-3-N(4)-benzilthiosemicarbazone (IBTC), which in our previous study proved to be an antioxidant and antiatherogenic molecule, could protect and reactivate AChE and BChE. Toxicity of IBTC after subcutaneous injection in mice was measured using assays for oxidized diclorofluoresceine (DCF), thiobarbituric acid reactive substances (TBARS), non-protein thiol (NPSH) levels, and catalase (CAT), sodium potassium (Na(+)/K(+)) ATPase, delta-aminolevulinic acid dehydratase (ALA-D), and glutathione peroxidases (GPx) enzyme activities. The cytotoxicity was evaluated and the enzymatic activity of cholinesterase was measured in human blood samples. Molecular docking was used to predict the mechanism of IBTC interactions with the AChE active site. We found that IBTC did not increase the amount of DCF-RS or TBARS, did not reduce NPSH levels, and did not increase CAT, (Na(+)/K(+)) ATPase, ALA-D, or GPx activities. IBTC protected and reactivated both AChE and BChE activities. Molecular docking predicted that IBTC is positioned at the peripheral anionic site and in the acyl binding pocket of AChE and can interact with methamidophos, releasing the enzyme's active site. Our results suggest that IBTC, besides being an antioxidant and a promising antiatherogenic agent, is a non-toxic molecule for methamidophos poisoning treatment.

show abstract

“…Ακάθμβα απμηεθέζιαηα ιε ιείςζδ ηδξ δναζηζηυηδηαξ ηδξ AChE παναηδνήεδηε ζε πθάζια αίιαημξ ιοχκ ιεηά απυ έηεεζδ ζε methamidophos (Zayed et al 1984).…”

Section: βηνρεκηθέο-ηζηνρεκηθέο παξαηεξήζεηοunclassified

Χρήση βιοσημάντων των βιοδεικτών coturnix coturnix japonica ( ορτύκι) και onchorhynchus mykiss (πέστροφα) για την αξιολόγηση οικοτοξικολογικών επιπτώσεων του methamidophos

Φουντουλάκης¹

View full text Add to dashboard Cite

Θέθς κα εηθνάζς ηζξ εοπανζζηίεξ ιμο πνμξ ημκ επζαθέπμκηα Καεδβδηή η. Γενάζζιμ Ανάπδ βζα ηδκ ακάεεζδ αοημφ ημο πνςηυηοπμο ηαζ εκδζαθένμκημξ εέιαημξ, ημ μπμίμ απμηεθεί ηαζκμφνβζμ ακηζηείιεκμ βζα ημ Δνβαζηήνζυ ηαζ ενέεζζια βζα πεναζηένς ένεοκα, ηαεχξ ηαζ βζα ηδκ άρμβδ ζοκενβαζία, ηζξ πνήζζιεξ ζοιαμοθέξ ηαζ ηδ δζυνεςζδ ηςκ ηεζιέκςκ. Δπίζδξ εέθς κα εοπανζζηήζς ηδκ Καεδβήηνζα ηα. Μανία Υνοζάβδ βζα ηδκ πνυεοιδ ζοκενβαζία ηδξ ζε υθα ηα εέιαηα πμο πνμέηορακ ηαζ βζα ηδκ επζιεθή δζυνεςζδ ηςκ ηεζιέκςκ ηαεχξ ηαζ ημκ Καεδβδηή η. Αοβμοθά Υνήζημ βζα ηζξ πνήζζιεξ ζοιαμοθέξ ημο ηαζ ηδ δζυνεςζδ ηςκ ηεζιέκςκ. Θα ήεεθα κα εοπανζζηήζς ζδζαίηενα ημ πνμζςπζηυ ηςκ δζαπζζηεοιέκςκ ενβαζηδνίςκ, Ecotoxicological Laboratory, Fácáncert ηαζ Laboratory of hydrobiology, Százhalombatta, ηδξ Οοββανίαξ βζα ηδκ πμθφηζιδ αμήεεζα ηαζ ηδκ πνυεοιδ ζοκενβαζία ημοξ βζα ηδ δζεκένβεζα ημο πεζναιαηζζιμφ ενβαζηδνίμο ιε ημοξ αζμδείηηεξ ηαεχξ ηαζ ηδ ζοθθμβή ζζηχκ. Σα ενβαζηήνζα αοηά έηθεζζακ ιε ηδκ είζμδμ ηδξ Οοββανίαξ ζηδκ ΔΔ. Θα ήεεθα κα εοπανζζηήζς ημοξ δζεοεοκηέξ ηαζ ημ πνμζςπζηυ ηςκ ενβαζηδνίςκ Ακαημιίαξ ηαζ Φοζζμθμβίαξ Εχςκ ηαζ Γεςνβζηήξ Φανιαημθμβίαξ βζα ηδ δζάεεζδ επζζηδιμκζηχκ μνβάκςκ ηαζ οθζηχκ ηαζ ηδ ζοκενβαζία ημοξ βζα ηδκ εηπυκδζδ ιένμξ ημο πεζναιαηζζιμφ. Σέθμξ εα ήεεθα κα εοπανζζηήζς ηδκ ηα Πακαβζχηα Μαθάημο απυ ημ Δνβ. Τπμθεζιιάηςκ Γεςνβ. Φανιάηςκ ημο Μπεκαηείμο Φοημπαεμθμβζημφ Ηκζηζημφημο βζα ηδκ πμθφηζιδ ζοιιεημπή ηδξ ζηδκ ακάθοζδ οπμθεζιιάηςκ ημο ζζηδνεζίμο ηςκ μνηοηζχκ. Θα ήεεθα, ζε αοηυ ημ ζδιείμ, κα εοπανζζηήζς ζδζαίηενα ημκ Δπίημονμ Καεδβδηή ημ Υνήζημ Μπαθάζηα βζα ηδ αμήεεζα πμο ιμο πανείπε, ηαε' υθδ ηδ δζάνηεζα ηδξ εηπυκδζδξ ηδξ πηοπζαηήξ ιμο ιεθέηδξ ηαζ ηδ δζυνεςζδ ηςκ ηεζιέκςκ. Ζ ζοκεζζθμνά ημο, ηυζμ ζε ζημζπεία, υζμ ηαζ ζε ροπμθμβζηή ζηήνζλδ ήηακ αιένζζηδ. Σέθμξ εα ήεεθα κα εοπανζζηήζς ημ πνμζςπζηυ ημο Δνβαζηδνίμο Οζημθμβίαξ ηαζ Δπζζηήιδξ Πενζαάθθμκημξ ηαζ Φοζζμθμβίαξ ηαζ Μμνθμθμβίαξ θοηχκ βζα ηδ ζοκενβαζία ημοξ ηαζ ημ ζοκαδεθθζηυ πκεφια, ηαεχξ ηαζ υθμοξ υζμζ ιε μπμζμδήπμηε ηνυπμ ζοκέααθακ ζηδκ μθμηθήνςζδ αοηήξ ηδξ ιεθέηδξ. Δοπανζζηχ απυ ηανδζάξ ηδκ μζημβέκεζά ιμο βζα ηδκ οπμζηήνζλδ ηαζ ηδκ οπμιμκή ημοξ. Δίκαζ υθμζ ημοξ εοθμβδιέκμ δχνμ ημο Θεμφ. Ζ πανμφζα δζαηνζαή πνδιαημδμηήεδηε ζηα πθαίζζα ημο πνμβνάιιαημξ «Πενζαάθθμκ -Ποεαβυναξ ΗΗ -Δκίζποζδ Δνεοκδηζηχκ Οιάδςκ ζημ Γ.Π.Α». V Λίζηα πληνκεύζεσλ πληνκ. Διιεληθά Αγγιηθά ΓΑ Μεηααμθή ηδξ απμννυθδζδξ Change in absorbance .Β. ςιαηζηυ αάνμξ Body weight ΦΠ Φοημπνμζηαηεοηζηυ πνμσυκ Plant Protection Product ACh Αηεηοθπμθίκδ Acetylcholine AChE Αηεηοθπμθζκεζηενάζδ Acetylcholinesterase ADME Απμννυθδζδ, ηαηακμιή, ιεηααμθζζιυξ ηαζ απέηηνζζδ Absorption, Distribution, Metabolism, Elimination ART Γζάνηεζα ημο πνυκμο έηθεζρδξ ηςκ ηθζκζηχκ ζοιπηςιάηςκ Acute recovery time a.s. Γναζηζηή μοζία Active substance ASCh Ηςδζμφπμξ αηεηοθμεεζμπμθίκδ Acetylthiocholine iodide AV οκηεθεζηήξ απμθοβήξ (1 = υπζ απμθοβή, 0 = πθήνδξ απμθοβή) Avoidance factor AVD Υνυκμξ ηαεοζηένδζδξ ηδξ απμθοβήξ ηδξ πνυζθδρδξ ηνμθήξ Avoidance delay time AVT Γυζδ μνίμο απμθοβήξ ηδξ πνυζθδρδξ ηνμθήξ Avoidance threshold bw ςιαηζηυ αάνμξ Body wei...

show abstract

Some toxicological aspects op methamidophos exposure in mice¹

Cited by 5 publications

References 3 publications

Analysis and fate of acephate and its metabolite, methamidophos, in pepper and cucumber

Analysis and fate of acephate and its metabolite, methamidophos, in pepper and cucumber

Isatin-3-N4-benzilthiosemicarbazone, a non-toxic thiosemicarbazone derivative, protects and reactivates rat and human cholinesterases inhibited by methamidophos in vitro and in silico

Χρήση βιοσημάντων των βιοδεικτών coturnix coturnix japonica ( ορτύκι) και onchorhynchus mykiss (πέστροφα) για την αξιολόγηση οικοτοξικολογικών επιπτώσεων του methamidophos

Contact Info

Product

Resources

About

Some toxicological aspects op methamidophos exposure in mice1

Cited by 5 publications

References 3 publications

Analysis and fate of acephate and its metabolite, methamidophos, in pepper and cucumber

Analysis and fate of acephate and its metabolite, methamidophos, in pepper and cucumber

Isatin-3-N4-benzilthiosemicarbazone, a non-toxic thiosemicarbazone derivative, protects and reactivates rat and human cholinesterases inhibited by methamidophos in vitro and in silico

Χρήση βιοσημάντων των βιοδεικτών coturnix coturnix japonica ( ορτύκι) και onchorhynchus mykiss (πέστροφα) για την αξιολόγηση οικοτοξικολογικών επιπτώσεων του methamidophos

Contact Info

Product

Resources

About

Some toxicological aspects op methamidophos exposure in mice¹