SoMYB18, a sugarcane MYB transcription factor improves salt and dehydration tolerance in tobacco

Shingote, Prashant Raghunath; Kawar, Prashant G.; Pagariya, Madhuri Chandrakant; Kuhikar, Rutuja Suresh; Thorat, A. S.; Babu, K. Harinath

doi:10.1007/s11738-015-1961-1

Cited by 35 publications

(22 citation statements)

References 52 publications

(71 reference statements)

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“…These TFs could be divided into 29 gene families based on their putative DNA-binding and kinase domains ( Figure 5 ); most of the members of the 13 TF gene families (zinc finger protein (ZF), WD40, AP2/EREBP, HB, bHLH, B3, MYB, NLP, NF-Y, TCP, bZIP, GATA, and NAC) were downregulated. Among these, at least six TF gene families have been reported to be linked to positive regulation of the responses to dehydration stress and comprise AP2/EREBP [ 36 ], bZIP [ 37 ], HB [ 38 ], MYB [ 39 ], NAC [ 35 ], and ZF [ 40 ]. Furthermore, it has also been reported that these six TF gene families are involved in drought stress resistance of tea plants [ 13 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptome and Expression Profiling Analysis of Recalcitrant Tea (Camellia sinensis L.) Seeds Sensitive to Dehydration

Jin

Liu

et al. 2018

International Journal of Genomics

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The tea plant (Camellia sinensis (L.) O. Kuntze) is an economically important woody perennial nonalcoholic health beverage crop. Tea seeds are categorized as recalcitrant and are sensitive to dehydration treatment. However, the molecular basis of this phenomenon has not been investigated. Thus, we analyzed the genome-wide expression profiles of three dehydration stages using RNA-Seq and digital gene expression (DGE) technologies. We performed de novo assembly and obtained a total of 91,925 nonredundant unigenes, of which 58,472 were extensively annotated. By a hierarchical clustering of differentially expressed genes (DEGs), we found that 8929 DEGs were downregulated and 5875 DEGs were upregulated during dehydration treatment. A series of genes related to ABA biosynthesis and signal transduction, transcription factor, antioxidant enzyme, LEA protein, and proline metabolism that have been reported to function in dehydration process were found to be downregulated. Additionally, the expression profiles of 12 selected genes related to tea seed dehydration treatment were confirmed by qRT-PCR analysis. To our knowledge, this is the first genome-wide study elucidating the possible molecular mechanisms of sensitivity of recalcitrant tea seeds to dehydration. The results obtained in this study contribute to the preservation of tea seeds as genetic resources and can also be used to explore the mechanism of dehydration sensitivity of other recalcitrant seeds.

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Section: Resultsmentioning

confidence: 99%

Transcriptome and Expression Profiling Analysis of Recalcitrant Tea (Camellia sinensis L.) Seeds Sensitive to Dehydration

Jin

Liu

et al. 2018

International Journal of Genomics

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“…In recent years, many studies have shown that TFs are essential in mediating salt stress response. For example, overexpression of NAC [ 39 ], MYB [ 40 ], heat shock transcription factor ( HSF ) [ 41 ], WRKY [ 42 ], and bHLH [ 43 ] transcription factors can significantly improve salt tolerance in plants. In this study, 39 genes homologous to NAC , MYB , HSF , WRKY , bHLH , ERF , homeo-box ( HB ), and other TFs , were significantly up-regulated by salt treatment ( Table 3 ).…”

Section: Resultsmentioning

confidence: 99%

Physiological and Transcriptomic Responses of Chinese Cabbage (Brassica rapa L. ssp. Pekinensis) to Salt Stress

Qiu

Liu

et al. 2017

IJMS

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Salt stress is one of the major abiotic stresses that severely impact plant growth and development. In this study, we investigated the physiological and transcriptomic responses of Chinese cabbage “Qingmaye” to salt stress, a main variety in North China. Our results showed that the growth and photosynthesis of Chinese cabbage were significantly inhibited by salt treatment. However, as a glycophyte, Chinese cabbage could cope with high salinity; it could complete an entire life cycle at 100 mM NaCl. The high salt tolerance of Chinese cabbage was achieved by accumulating osmoprotectants and by maintaining higher activity of antioxidant enzymes. Transcriptomic responses were analyzed using the digital gene expression profiling (DGE) technique after 12 h of treatment by 200 mM NaCl. A total of 1235 differentially expressed genes (DEGs) including 740 up- and 495 down-regulated genes were identified. Functional annotation analyses showed that the DEGs were related to signal transduction, osmolyte synthesis, transcription factors, and antioxidant proteins. Taken together, this study contributes to our understanding of the mechanism of salt tolerance in Chinese cabbage and provides valuable information for further improvement of salt tolerance in Chinese cabbage breeding programs.

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“…Many members in plant R2R3-MYB family have been reported as abiotic-stress-induced transcription factors 40 41 42 43 . Moreover, over-expressing the R2R3-MYB genes improved the resistance to freezing, drought, and salt stresses in transgenic plants 44 45 46 . In this study, ScMYB2S2 , one alternatively spliced transcript of ScMYB2 , was induced at the late periods of PEG-simulated drought stress.…”

Section: Discussionmentioning

confidence: 99%

A sugarcane R2R3-MYB transcription factor gene is alternatively spliced during drought stress

Guo

Ling

et al. 2017

Sci Rep

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MYB transcription factors of the R2R3-MYB family have been shown to play important roles in many plant processes. A sugarcane R2R3-MYB gene (ScMYB2) and its two alternative forms of transcript (ScMYB2S1 and ScMYB2S2) were identified in this study. The deduced protein of ScMYB2S1 is a typical plant R2R3-MYB protein, while ScMYB2S2 encodes a truncated protein. Real-time qPCR analysis revealed that ScMYB2S1 is suppressed under PEG-simulated drought stress in sugarcane, while ScMYB2S2 is induced at later treatment stage. A senescence symptom was observed when ScMYB2S1 was injected into tobacco leaves mediated by Agrobacterium, but no symptom for ScMYB2S2. Further investigation showed that the expression levels of 4 senescence-associated genes, NtPR-1a, NtNYC1, NtCAT3 and NtABRE, were markedly induced in tobacco leaves after ScMYB2S1-injection, while they were not sensitive to ScMYB2S2-injection. Moreover, MDA and proline were also investigated after injection. Similarly, MDA and proline levels were induced by ABA and ScMYB2S1, while inhibited by ScMYB2S2. We propose that ScMYB2, by alternatively splicing two transcripts (ScMYB2S1 and ScMYB2S2), is involved in an ABA-mediated leaf senescence signaling pathway and play positive role in respond to drought-induced senescence in sugarcane. The results of this study provide information for further research in sugarcane stress processes.

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SoMYB18, a sugarcane MYB transcription factor improves salt and dehydration tolerance in tobacco

Cited by 35 publications

References 52 publications

Transcriptome and Expression Profiling Analysis of Recalcitrant Tea (Camellia sinensis L.) Seeds Sensitive to Dehydration

Transcriptome and Expression Profiling Analysis of Recalcitrant Tea (Camellia sinensis L.) Seeds Sensitive to Dehydration

Physiological and Transcriptomic Responses of Chinese Cabbage (Brassica rapa L. ssp. Pekinensis) to Salt Stress

A sugarcane R2R3-MYB transcription factor gene is alternatively spliced during drought stress

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