Sonic Hedgehog Is a Remotely Produced Cue that Controls Axon Guidance Trans-axonally at a Midline Choice Point

Peng, Jimmy; Fabre, Pierre J.; Dolique, Tiphaine; Swikert, Shannon M.; Kermasson, Laëtitia; Shimogori, Tomomi; Charron, Frédéric

doi:10.1016/j.neuron.2017.12.028

Cited by 71 publications

(67 citation statements)

References 60 publications

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“…In the mouse, approximately 5% of neurons are excluded from the optic chiasm and form an ipsilateral projection in the optic tract (Petros et al, 2008). These ipsilateral axons are repelled by a combination of EphrinB2 expressed by optic chiasm glial cells, and Shh secreted transaxonally by contralateral RGC axons (Peng et al, 2018; Williams et al, 2003). Additional molecular cues govern axonal sorting, targeting of specific retinorecipient regions by subsets of RGC axons, and retinotopic mapping after crossing the chiasm (Feldheim et al, 1998; Hindges et al, 2002; Osterhout et al, 2011; Schmitt et al, 2006; Su et al, 2011; Suetterlin and Drescher, 2014; Sun et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Retinal Ganglion Cell Axon Sorting at the Optic Chiasm Requires Dystroglycan

Clements

Wright

2018

Preprint

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Statement 1Abnormal retinal ganglion cell axon sorting in the optic chiasm in the absence of 2 functional dystroglycan results in profound defects in retinorecipient innervation. 3 4 Abstract 5In the developing visual system, retinal ganglion cell (RGC) axons project 6 from the retina to several distal retinorecipient regions in the brain. Several 7 molecules have been implicated in guiding RGC axons in vivo, but the role of 8 extracellular matrix molecules in this process remains poorly understood. 9Dystroglycan is a laminin-binding transmembrane protein important for formation 10 and maintenance of the extracellular matrix and basement membranes and has 11 previously been implicated in axon guidance in the developing spinal cord. Using 12 two genetic models of functional dystroglycan loss, we show that dystroglycan is 13 necessary for correct sorting of contralateral and ipsilateral RGC axons at the 14 optic chiasm. Missorted axons still target retinorecipient brain regions and persist 15 in adult mice, even after axon pruning is complete. Our results highlight the 16 importance of the extracellular matrix for axon sorting at an intermediate choice 17 point in the developing visual circuit. 18 19

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Section: Introductionmentioning

confidence: 99%

Retinal Ganglion Cell Axon Sorting at the Optic Chiasm Requires Dystroglycan

Clements

Wright

2018

Preprint

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“…Their expression may be more restricted later on during RGC development (Pcdh9), or expand to other RGC cell types (Slc6a4; (Assali et al, 2017)). This latter gene is specific to the RGCs that will send their axon to the ipsilateral side of the brain (Koch et al, 2011;Peng et al, 2018). Overall, this analysis showed us that, at this stage (E15), we can recover a large spectrum of differentiation stages of an early retinal cell type.…”

Section: Transcriptional Waves Drive Retinal Ganglion Cell Differentimentioning

confidence: 66%

“…7F), it may also have similar targets and thus play a role in the specification of ipsilateral RGCs. Of note, we fail to detect other genes involved in ipsilateral RGC guidance, such as Boc (Fabre et al, 2010;Peng et al, 2018) andFoxD1 (Carreres et al, 2011, 1;Herrera et al, 2004), in a sufficient number of cells. Along with Zic2, these genes might be either expressed at levels below detection, or be subject to high drop-out events, a caveat of the drop-seq procedure.…”

Section: Identification Of Ipsilateral Rgc Signaturesmentioning

confidence: 67%

Transcriptional logic of cell fate specification and axon guidance in early born retinal neurons revealed by single-cell mRNA profiling

Giudice

Leleu

Fabre

2018

Preprint

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Retinal ganglion cells (RGC), together with cone photoreceptors, horizontal cells (HC) and amacrine cells (AC), are the first classes of neurons produced in the retina. Here we have profiled 5348 single retinal cells and provided a comprehensive transcriptomic atlas showing the broad diversity of the developing retina at the time when the four early-born cells are being produced. Our results show the transcriptional sequences that establish the hierarchical ordering of early cell fate specification in the retina. RGC maturation follows six waves of gene expression, giving new insight into the regulatory logic of RGC differentiation. Early-generated RGCs transcribe an increasing amount of guidance cues for young peripheral RGC axons that express the matching receptors. Finally, spatial signatures in sub-populations of RGCs allowed to define novel molecular markers that are spatially restricted during the development of the retina. Altogether this study is a valuable resource that identifies new players in mouse retinal development, shedding light on transcription factors sequence and guidance cues dynamics in space and time.Butler, A., Hoffman, P., Smibert, P., Papalexi, E. and Satija, R. (2018). Integrating single-cell transcriptomic data across different conditions, technologies, and species. Nature Biotechnology 36, 411-420.Cang, J., Wang, L., Stryker, M. P. and Feldheim, D. A. (2008). Roles of ephrin-as and structured activity in the development of functional maps in the superior colliculus.

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“…In addition, the transcription factor Forkhead box D1 was shown to be critical in maintaining iRGCs fate by promoting the expression of Zic2 (Herrera ). In addition to the EphB1/ephrin‐B2 repulsion pathway, another pathway also controls ipsilateral RGC repulsion: Shh is expressed by contralateral RGCs and transported axonally and anterogradely to the optic chiasm (Peng et al ). At the optic chiasm, ipsilateral RGCs, which express the Shh receptor Boc, are repelled by Shh and therefore do not cross the optic chiasm, remaining ipsilateral (Fabre et al ; Peng et al ) (Fig.…”

Section: The Developing Nervous Systemmentioning

confidence: 99%

Construction and reconstruction of brain circuits: normal and pathological axon guidance

Roig‐Puiggros

Vigouroux

Beckman

et al. 2019

Journal of Neurochemistry

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Perception of our environment entirely depends on the close interaction between the central and peripheral nervous system. In order to communicate each other, both systems must develop in parallel and in coordination. During development, axonal projections from the CNS as well as the PNS must extend over large distances to reach their appropriate target cells. To do so, they read and follow a series of axon guidance molecules. Interestingly, while these molecules play critical roles in guiding developing axons, they have also been shown to be critical in other major neurodevelopmental processes, such as the migration of cortical progenitors. Currently, a major hurdle for brain repair after injury or neurodegeneration is the absence of axonal regeneration in the mammalian CNS. By contrasts, PNS axons can regenerate. Many hypotheses have been put forward to explain this paradox but recent studies suggest that hacking neurodevelopmental mechanisms may be the key to promote CNS regeneration. Here we provide a seminar report written by trainees attending the second Flagship school held in Alpbach, Austria in September 2018 organized by the International Society for Neurochemistry (ISN) together with the Journal of Neurochemistry (JCN). This advanced school has brought together leaders in the fields of neurodevelopment and regeneration in order to discuss major keystones and future challenges in these respective fields.

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Sonic Hedgehog Is a Remotely Produced Cue that Controls Axon Guidance Trans-axonally at a Midline Choice Point

Cited by 71 publications

References 60 publications

Retinal Ganglion Cell Axon Sorting at the Optic Chiasm Requires Dystroglycan

Retinal Ganglion Cell Axon Sorting at the Optic Chiasm Requires Dystroglycan

Transcriptional logic of cell fate specification and axon guidance in early born retinal neurons revealed by single-cell mRNA profiling

Construction and reconstruction of brain circuits: normal and pathological axon guidance

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